Functional analyses and regulation of the metabolism of tissue microenvironmental factors by metalloproteinases

金属蛋白酶对组织微环境因子代谢的功能分析和调节

• 批准号: 19109004
• 负责人: OKADA Yasunori
• 金额: $ 74.63万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (S)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19109004/
• 关键词: MMP; ADAM; ノックアウトマウス; 浸潤; 転移; 創傷治癒; 変形性関節症; ヒアルロン酸; エンドスタチン; 動脈瘤; セマフォリン; ELISA; 癌転移; 血管新生; ADAMTS4; アグリカナーゼ; IL-1; CD44; カルシウムペントサン硫酸; 筋線維芽細胞; ヘパラナーゼ; 腎癌

We searched for interacting molecules for members of the MMP (matrix metalloproteinase) and the ADAM (a disintegrin and metalloproteinase) gene families, and investigated roles of these metalloproteinases in metabolism of tissue microenvironmental factors in cancers and non-neoplastic diseases with an aim of developing novel diagnostic and/or therapeutic tools. Our studies provided progresses in purification of several proMMPs, roles of MMPs in skin wound healing, implications of ADAM28 in cancer cell proliferation and metastasis, development of a measuring method for ADAM28, and roles of ADAMTS4 and ADAM12 in destruction and repair of osteoarthritic cartilage.

我们搜索了MMP（基质金属蛋白酶）和ADAM（一种崩解蛋白和金属蛋白酶）基因家族的相互作用分子，并研究了这些金属蛋白酶在组织中微塑性因子的代谢中这些金属蛋白酶在癌症和非抑郁症工具中具有新颖诊断和或或不断诊断的工具的作用。我们的研究提供了净化几个Promps，MMP在皮肤伤口愈合中的作用，ADAM28在癌细胞增殖和转移中的影响，ADAM28的测量方法的开发以及ADAMTS4和ADAM12在破坏和修复骨关节炎骨软骨中的作用的进展。

项目成果

Periostin advances atherosclerotic and rheumatic cardiac valve degeneration by inducing angiogenesis and MMP production in humans and rodents

• DOI: 10.1172/jci40973
• 发表时间: 2010-07-01
• 期刊: JOURNAL OF CLINICAL INVESTIGATION
• 影响因子: 15.9
• 作者: Hakuno, Daihiko;Kimura, Naritaka;Fukuda, Keiichi
• 通讯作者: Fukuda, Keiichi

Brain-specific expression of vascular endothelial growth factor 146 correlates with the blood-brain barrier induction in quail embryos

鹌鹑胚胎中血管内皮生长因子 146 的脑特异性表达与血脑屏障诱导相关

• 发表时间: 2008
• 期刊: Dev. Neurosci. 30
• 作者: Ikeda E.;Kodama T. Okada Y.
• 通讯作者: Kodama T. Okada Y.

Substrates and roles of ADAM28 in human cancers. Gordon Research Conference on Proteolytic Enzymes and Their Inhibitors

ADAM28 在人类癌症中的底物和作用。

• 发表时间: 2010
• 作者: 松本佳巳;西野邦彦;山口明人;辻本豪三;中務真人;Yasunori Okada
• 通讯作者: Yasunori Okada

Proteinases and matrix degradation. In Kelley's Textbook of Rheumatology

蛋白酶和基质降解。

• 发表时间: 2012
• 作者: Okada Y;Firestein G. S.;Budd R. C.;Gabriel S. E.;McInnes I. B. and O' Dell J. R
• 通讯作者: McInnes I. B. and O' Dell J. R

ADAM28. In : Handbook of Proteolytic Enzymes

亚当28。

• 发表时间: 2012
• 作者: Yabu;M.;Shime;H.;et al;Mochizuki S. and Okada Y Rawlings N. D. and Salvesen G
• 通讯作者: Mochizuki S. and Okada Y Rawlings N. D. and Salvesen G