Development of Nitroarene Analyzer

硝基芳烃分析仪的研制

基本信息

批准号：
06557121
负责人：
HAYAKAWA Kazuichi
金额：
$ 3.58万
依托单位：
Kanazawa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research (B)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

(1) Both the nitroarene analyzer and the Ames test are necessary in the study on mutagenic nitroarenes. For the simultaneous use of these two methods, several solvent systems were tested to extract 1,3-dinitropyrene (1,3-DNP), 1,6-DNP,1,8-DNP and 1-nitropyrene (1-NP) as well as directacting mutagenic activity from airborne particulates. Benzene-ethanol, acetonitrile, ethanol and methanol gave the high efficiencies. Considering toxicities, ethanol seemed to be the best solvent.(2) Although the nitroarene analyzer determined trace levels of 1,3-, 1,6-and 1,8-DNPs and 1-NP in extracts from a few mg of airborne particulates, the reduction of nitroarenes to corresponding aminoarenes was required before injection. To remove this tedious treatment, a reducer column packed with zinc/glass beads was on-line introduced into the system. Considering the better resolution of aminoarenes, both the reducer column and a switching valve equipped with a heart-cutting loop were introduced just before the … More ODS separation column. This system could determine the above four compounds in extracts from airborne particulatates without pre-column reduction.(3) In the method, not only nitroarenes but also polycyclic aromatic hydrocarbons (PAHs) were quantitatively extracted from airborne partculates. To determine them simultaneously, both the nitroarene analyzer and a conventional high-performance liquid chromatograph with a fluorescence detector were combined using a switching valve. After the reduced sample solution was injected, four nitroarenes and eight PAHs were separately determined in the two different lines in 1h with high sensitivities.(4) Several mutagenic nitroarenes such as 2-, 4-NPs, 2-nitrofluoranthene and 6-nitrochrycene were formed in air in the presence of NOx. To determine these compounds, a reducer column packed with zinc/glass beads and two ODS columns, one for the clean-up and the other for the concentration, were introduced into the nitroarene analyzer. By the system, the above four compounds were detected in air. The fact that their ratios to 1,3-, 1,6-, 1,8-DNPs and 1-NP in rural air were larger than those in urban air suggested the formation of those compounds during the transportation. However, their mutagenic contributions were not large. Less

(1)硝基芳烃分析仪和Ames试验都是诱变硝基芳烃研究中必需的。为了同时使用这两种方法，测试了几种溶剂系统提取1,3-二硝基芘(1,3-DNP)，1。 ,6-DNP、1,8-DNP 和 1-硝基芘 (1-NP) 以及空气中颗粒物的直接诱变活性。苯乙醇、乙腈、乙醇和甲醇的效率较高，考虑到毒性，乙醇似乎是最好的溶剂。(2)尽管硝基芳烃分析仪测定了痕量的1,3-、1,6-和1,8-。从几毫克空气颗粒物中提取的 DNP 和 1-NP，在注射前需要将硝基芳烃还原为相应的氨基芳烃以将其除去。为了进行繁琐的处理，将填充有锌/玻璃珠的还原柱在线引入到系统中，考虑到氨基芳烃的更好分离度，在系统之前引入了还原柱和配备中心切割环的切换阀。该系统无需柱前还原即可测定空气颗粒物提取物中的上述四种化合物。(3)该方法不仅可以检测硝基芳烃，还可以检测多环芳烃(PAHs)。将硝基芳烃分析仪与带荧光检测器的传统高效定量液相色谱仪结合使用，注入还原后的样品溶液，分别测定四种硝基芳烃和八种多环芳烃。 1 小时内在两个不同的系中检测，灵敏度高。(4) 几种致突变硝基芳烃，如 2-、4-NPs、 2-硝基荧蒽和 6-硝基苯在存在 NOx 的情况下在空气中形成。为了测定这些化合物，使用了装有锌/玻璃珠的还原剂柱和两个 ODS 柱，一个用于净化，另一个用于浓缩。引入硝基芳烃分析仪，检测空气中上述四种化合物的比例。农村空气中的 1-NP 含量大于城市空气中的含量，表明这些化合物是在运输过程中形成的，但其致突变作用并不大。