Development and Application of chiral separation HPLC methods with fluorescence or chemiluminescence detection for determination of metamphetamines

荧光或化学发光检测手性分离高效液相色谱法测定甲基苯丙胺的开发及应用

• 批准号: 11672145
• 负责人: NAKASHIMA Kenichiro
• 金额: $ 2.24万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11672145/
• 关键词: Abuse drugs; methamphetamines; fluorescence detection; high-performance liquid chromatography; chiral separation; semi-micro column HPLC; chemiluminescence detection; ロフィン誘導体

A highly sensitive HPLC method for the determination of methamphetamines (MPs) has been developed using a newly synthesized fluorescent reagent, 4-(4,5-diphenyl-1H-imidazol-2-yl)benzoyl chloride (DIB-Cl) as a label. DIB derivatives of MPs, e.g., metamphetamine, amphetamine, p-hydroxymethamphetamine, and ephedrine, were separated and detected with peroxyoxalate chemiluminescence (PO-CL) and fluorescence methods. Fluorescence detection was more sensitive than PO-CL, thus HPLC with fluorescence detection was selected in this study. The developed method could detect methamphetamines at amol〜fmol levels. Some abusers' urine and hair samples were successfully determined by the method. We also focused ourselves to develop simple and economic methods by downsizing the system using semi-micro column HPLC.Achiral and chiral semi-micro column HPLC methods have been developed, which could be successfully applied to the determination of MPs in only 10 μL of urine or 1 cm-long hair samples.The proposed methods are simple and sensitive, and thus, they would be useful for pharmacological, toxicological and pharmaceutical studies, especially for the studies with samples including trace amounts of MPs.

使用新合成的荧光试剂 4-(4,5-二苯基-1H-咪唑-2-基)苯甲酰氯 (DIB-Cl) 作为标记，开发了一种测定甲基苯丙胺 (MP) 的高灵敏度 HPLC 方法MP 的 DIB 衍生物，例如甲基苯丙胺、苯丙胺、对羟基甲基苯丙胺和麻黄碱，可通过以下方法分离和检测：过氧草酸盐化学发光法（PO-CL）和荧光检测法比 PO-CL 法更灵敏，因此本研究选择采用荧光检测法的 HPLC 来检测部分滥用者尿液和头发中的甲基苯丙胺。我们还致力于通过使用半微柱 HPLC 缩小系统规模来开发简单且经济的方法。非手性和手性半微柱 HPLC 方法已经开发出来，该方法可成功应用于仅 10 μL 尿液或 1 cm 长头发样品中 MP 的测定。所提出的方法简单且灵敏，因此可用于药理学、毒理学和药物研究，特别是对于对含有微量 MP 的样品进行研究。

项目成果

O.Al-Dirbashi,et al.: "HPLC with fluorescence detection of methamphetamine and amphetamine in segmentally analyzed human hair"Analyst. 124. 493-497 (1999)

O.Al-Dirbashi 等人：“HPLC 荧光检测分段分析的人发中的甲基苯丙胺和苯丙胺”分析师。

O.Al-Dirbashi, et al.: "Enantiomer-specific high-performance liquid chromatography with fluorescence detection of methamphetamine......"Biomedical Chromatography. 13. 543-547 (1999)

O.Al-Dirbashi 等人：“对映体特异性高效液相色谱法与荧光检测甲基苯丙胺……”生物医学色谱法。

O.Y.Al-Dirbashi, et al.: "Quantification of methamphetamine, amphetamine and enantiomers by semi-micro column HPLC with fluorscence detection ..."Biomedical Chromatography. 14. 293-300 (2000)

O.Y.Al-Dirbashi 等人：“通过半微柱 HPLC 和荧光检测对甲基苯丙胺、苯丙胺和对映体进行定量……”生物医学色谱法。

N.Kuroda,K.Nakashima: "Reagent for CL Detection in "Modern Derivatization Methods for Separation Sciences" ed. by T. Toyo'oka"John Wiley & Sons Ltd.. 22 (1999)

N.Kuroda、K.Nakashima：“《分离科学的现代衍生化方法》编辑中的 CL 检测试剂”，T. Toyooka 编着”John Wiley

O.Y.Al-Dirbashi, et al.: "Achiral and chiral quantification of methamphetamine and amphetamine in human urine by semi-micro column high-performance liquid....."Journal of Forensic Sciences. 45(3). 708-714 (2000)

O.Y.Al-Dirbashi 等人：“通过半微柱高性能液体对人尿液中的甲基苯丙胺和苯丙胺进行非手性和手性定量......”法医学杂志。