DETERMINATION OF MECHANISM FOR MOLECULAR ADHESION TO SOFT TISSUES AND MATERALS PREPARATION

软组织分子粘附机制的测定及材料制备

基本信息

批准号：
17300144
负责人：
ISHIHARA Kazuhiko
金额：
$ 9.98万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17300144/
关键词：
Adhesion Biomaterials Medical adhesives Polymer complex Physical treatment 医療接着材分子間相互作用

项目摘要

In this study the polymer structures were determined to adhere the polymer to tissues. The molecular design of the polymers were carried out with attention to the regulation of cell/materials interactions from of the physical chemistry and the biological both sides. The polymers could easily solidify themselves by the phase transition of the polymers and voluntary organization as far the normal temperature and to realize the adhesion prescribed it. It prescribed the molecular structure of this polymers closely and composed it, and the wound produced the soft tissue adhesion materials of the prototype than adhesion performance and examination of the safety.A thought prepared nanoparticles as a probe for recognizing the specific reaction analytical technique that I found so far; and to prescribe the functional group, which could adhere for a cell, the structure of the ligand molecule. At first the functional groups with various hydrophobicity, electric charge, hydrogen bonding connected to use physicochemical interaction. The water-soluble phospholipid polymers, which did immobilization of the specific functional group was used for surface treatment materials and the polymer solution for making an O/W emulsion and manufactured nanoparticles.The functional groups cold introduce with high-density for suitable interaction with cells. The adhesion of cells and tissues could be achieved. To determine the structure and composition bonded this polymer to the cells, which became single-layered and examined the reply of the cell As well as a form, the number of the increases, the intracellular enzyme activity was measured to consider in particular cell activity It prescribed polymer composition, the chemical structure that did not affect a cell than these. On the other hand, a molecule design about the polymers was carries out to reduce adhesive property and performed examination form the viewpoint of prevention of organization adhesion.

在这项研究中，确定了聚合物结构以将聚合物粘附到组织上。聚合物的分子设计注重从物理化学和生物两方面对细胞/材料相互作用的调节。在常温下，聚合物很容易通过聚合物的相变和自发组织而自行固化，并实现规定的粘合。详细规定了该聚合物的分子结构并对其进行了组合，在伤口上制作了原型的软组织粘合材料，比粘合性能和安全性进行了检查。一个想法是制备纳米颗粒作为识别特定反应分析技术的探针。到目前为止发现；并规定了可以粘附于细胞的官能团、配体分子的结构。首先将具有各种疏水性、电荷、氢键的官能团利用物理化学相互作用连接起来。固定了特定官能团的水溶性磷脂聚合物用于表面处理材料和用于制备O/W乳液和制造纳米粒子的聚合物溶液。以高密度冷引入官能团以与细胞适当相互作用。可以实现细胞和组织的粘附。为了确定该聚合物与细胞结合的结构和组成，细胞变成单层并检查细胞的应答以及形式、增加的数量，测量细胞内酶活性以特别考虑细胞活性这些规定的聚合物成分、化学结构不影响细胞。另一方面，对聚合物进行了分子设计以降低粘附性，并从防止组织粘附的角度进行了研究。