Structure Control and Functions of Biocompatible Polymer Alloy as Biomaterials

生物相容性聚合物合金作为生物材料的结构控制和功能

基本信息

批准号：
13480288
负责人：
ISHIHARA Kazuhiko
金额：
$ 9.41万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2004
项目状态：
已结题

项目摘要

In this research, polymer alloys composed of conventional polymer materials, such as, polyurethane and polysulfone, polyolefine and newly designed phospholipid polymers were researched as follows :To improve the biocompatibility of a segmented polyurethane (SPU), 2-methacryloyloxyethyl phosphorylcholine (MPC) copolymer was blended in the SPUs, such as Pellethane【○!R】 and TM-3【○!R】, by a solvent evaporation method from a homogeneous solution containing both SPU and MPC copolymer. X-ray electron spectra(XPS) indicated that the MPC moieties were located at the surface of the SPU membrane blended with the MPC copolymer even when the composition of the MPC copolymer was only 7.5 w/w % against the matrix SPU. The mechanical property of the SPU membranes, as determined by tensile stress-strain measurements, changed very little by addition of the MPC copolymer. Biocompatibility of the MPC copolymer blended membrane was evaluated by plasma protein adsorption and blood cell adhesion on the surfa … More ce. The amount of fibrinogen adsorbed on the SPU membrane significantly decreased by addition of the MPC copolymers. Moreover, addition of MPC copolymer in the SPU membrane suppressed platelet adhesion and activation. It is concluded that the blending of the MPC copolymer in the SPU membrane is an effective method to improve biocompatibility of the SPU membrane.Nonfouling polysulfone hollow fiber membranes from protein adsorption and deposition were newly developed by addition of MPC polymer. To improve hydrophilicity, permeability, and nonfouling characteristics of the PSf hollow fiber in a hemodialyzer, we synthesized a MPC polymer, which can be blended with PSf for preparing the polymer alloy (PSf/MPC polymer). The composition of the MPC polymer blended in the PSf was in the range between 7.0 and 15-wt%. From the PSf/MPC polymer solution, flat membranes and hollow fiber could be prepared. These membranes took an asymmetric structure and its mechanical strength was good. The surface characterization of the PSf/MPC polymer hollow fiber membrane by XPS revealed that the MPC units were concentrated at the surface. The permeability for solutes through the PSf/MPC polymer membrane was higher and the amount of protein adsorbed on the PSf/MPC polymer membrane was lower than those of the PSf membrane. Moreover, platelet adhesion was also effectively inhibited on the PSf/MPC polymer membrane Less

在本研究中，对由常规聚合物材料（例如聚氨酯和聚砜、聚烯烃以及新设计的磷脂聚合物）组成的聚合物合金进行了如下研究：为了提高嵌段聚氨酯（SPU）、2-甲基丙烯酰氧基乙基磷酰胆碱（MPC）共聚物的生物相容性混合在Pellethane【○!R】等SPU中TM-3【○！R】，通过溶剂蒸发法从含有SPU和MPC共聚物的均匀溶液中提取，X射线电子光谱（XPS）表明MPC部分位于与SPU共混的SPU膜的表面。 MPC 共聚物，即使 MPC 共聚物的组成相对于基质 SPU 仅为 7.5 w/w % SPU 膜的机械性能，通过拉伸测定。应力-应变测量，通过添加 MPC 共聚物改变很小。通过血浆蛋白吸附和表面上的血细胞粘附来评估 MPC 共聚物混合膜的生物相容性。SPU 膜上吸附的纤维蛋白原的量显着减少。此外，在 SPU 膜中添加 MPC 共聚物可以抑制血小板的粘附和活化。在SPU膜中添加MPC共聚物是提高SPU膜生物相容性的有效方法。通过添加MPC聚合物，新开发了具有蛋白质吸附和沉积作用的防污聚砜中空纤维膜，以提高PSf中空纤维的亲水性、渗透性和防污特性。在血液透析器中，我们合成了MPC聚合物，其可以与PSf共混以制备聚合物合金（PSf/MPC聚合物）的组合物。 PSf中MPC聚合物的掺混量为7.0-15wt%，由PSf/MPC聚合物溶液可以制备出不对称结构的平板膜和中空纤维膜，且其机械强度良好。通过 XPS 对 PSf/MPC 聚合物中空纤维膜的表面表征表明，MPC 单元集中在表面，溶质通过 PSf/MPC 聚合物膜的渗透性较高，蛋白质含量较高。 PSf/MPC聚合物膜上的吸附量低于PSf膜，而且PSf/MPC聚合物膜上的血小板粘附也得到有效抑制。