Mucus secretion studies using anti-gastric mucin monoclonal antibodies

使用抗胃粘蛋白单克隆抗体进行粘液分泌研究

基本信息

批准号：
07670612
负责人：
ISHIHARA Kazuhiko
金额：
$ 1.15万
依托单位：
Kitasato University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670612/
关键词：
anti-mucin antibody monoclonal antibody gastric mucin mucus secretion carbohydrate antigen 糖鐘抗原

项目摘要

Gastric mucin known as a major component of rat gastric mucus is synthesized in and secreted from several different kind of cells present at the mucosal epithelium. It is generally accepted that the regulation mechanism on the secretion and synthesis of mucin in a kind of cells present at the specific region and layr of gastric mucosa differ from the others. It is important to recognaize and estimate the specific mucin of gastric mucosa for understanding the role of mucus in the physiology and pathology of gastric mucosa. In this study we developed several monoclonal antibodies reacting specific mucin molecules and applied these antibodies to establish some methods to study the metabolism of gastric mucins.1) RGM11,21 and 24, are the monoclonal antibodies specifically react with the mucin present at the surface epithelial cells of rat gastric mucosa. It has been demonstrated that RGM11 and 21 recognize the specific oligosaccharide structure of mucin molecule. RGM24 reacts intact mucin molecules but the reactin disappears by the removal of saccharide residue from the mucin molecules.2) We developed 14 novel monoclonal antibodies (MAbs) using purified human colonic mucin as the immunogens (designated as HCM series MAbs). Among these MAbs, 8 MAbs are proved to recognize sialic acid-containing oligosaccaride structure of mucin molecule. In addition, some of these MAbs binding to sialic acid containing sugar chains are specifically react with the intestinal metaplastic mucosa in human stomach.3) We developed a sandwich ELISA mehtod using RGM11 and HIK1083 MAbs to recognize and quantify the surface mucous cell- and gland mucous cell-derived mucin, respectively, included in an extract of single mucosal specimen of rat stomach.

胃粘蛋白被称为大鼠胃粘液的主要成分，并从粘膜上皮的几种不同类型的细胞中分泌。人们普遍认为，在特定区域和胃粘膜的某种细胞中的分泌和合成的调节机制与其他细胞不同。重要的是要重毒和估计胃粘膜的特异性粘蛋白，以了解粘液在胃粘膜生理和病理学中的作用。在这项研究中，我们开发了几种反应特定粘蛋白分子的单克隆抗体，并应用了这些抗体来建立一些方法来研究胃粘蛋白的代谢。1）RGM11,21和24是单克隆抗体，是单克隆抗体，与粘液抗体特异性反应特异性地存在于表面上皮细胞的表面上皮细胞的gasicos mucosa。已经证明RGM11和21识别粘蛋白分子的特异性寡糖结构。 RGM24反应完整的粘蛋白分子，但反应素通过从粘蛋白分子中去除糖水残基消失。2）我们使用纯化的人类结肠粘蛋白作为免疫原（指定为HCM系列MABS）开发了14种新型的单克隆抗体（MABS）。在这些mAb中，事实证明，8个单克隆抗体可以识别粘蛋白分子的含有唾液酸的寡糖结构。此外，这些mAb中的某些mAb与含糖链结合的某些mAb特异性与人类胃中的肠道化生粘膜反应。3）我们使用RGM11和HIK1083 mAbs开发了一种三明治Elisa Mehtod，以识别和量化表面粘膜细胞和粘液粘液含量的单个Muccin，包括单个Muccin的表面粘膜和粘膜均应均等。