Experimental studies on application of anti-adhesion therapy for retinal microcirculatory disturbances

抗粘连治疗视网膜微循环障碍的实验研究

基本信息

批准号：
16390501
负责人：
OGURA Yuichiro
金额：
$ 8.7万
依托单位：
NAGOYA CITY UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

项目摘要

Leukocyte adhesion to the human retinal vascular endothelial cells was studied in vitro. High glucose concentration and insulin in the culture medium increased the adhesion of neutrophils to the retinal vascular endothelial cells. One of the adhesion molecules of the endothelial cells, intercellular adhesion molecule-1 (ICAM-1), was up-regulated by the high glucose and insulin. The leukocyte adhesion was inhibited by anti-ICAM-1 antibody and the inhibitor of protein kinase C. These results suggested that both glucose and insulin increased the expression of ICAM-1 of the retinal vascular endothelial cells through the activation of protein kinase C and that the increased expression of ICAM-1 is the cause for the adhesion of netrophils.The effect of triamcinolone acetonide on the leukocyte adhesion after scatter retinal photocoagulation was studied in rats in vivo. Acridine orange fluorography was used to study leukocyte dynamics in the retina after laser treatment. The rolling of leukocy … More tes along the retinal veins and increased leukocyte entrapment were observed not only in the photocoagulated area but also in the untreated retina. The OCT study showed the development of retinal edema in the untreated area. The temporal increase in the level of VEGF in the retina and choroid was detected. These results suggest that temporal increase in the VEGF in the retina and choroid by scatter photocoagulation enhances retinal leukostasis which leads to development of retinal edema. We also studied the effect of sub-Tenon's injection of triamcinolone acetonide on the enhanced leukocyte-endothelial interaction and retinal edema after scatter retinal photocoagulation in rats. The results suggested that triamcinolne effectively inhibited the rolling and entrapment of leukocytes in the retina and improved the retinal edema after scatter photocoagulation. This supports the use of triamcinolone acetonide for patients with macular edema to prevent the worsening of macular edema after panretinal laser photocoagulation. Less

在体外研究了白细胞粘附于视网膜的跨性别细胞。 EIN激酶C.这些结果表明，葡萄糖和胰岛素都会增加视网膜血管宇宙细胞的表达-1的激活，而ICAM -1的表达增加是Netrophil的原因。在体内研究了散射的视网膜光凝后，散布视网膜光凝后的白细胞粘附于体内，研究了视网膜的白细胞动力学。视网膜研究表明，视网膜中的VEGF水平暂时增加。在大鼠中，注射的乙炔是骗子的相互作用和视网膜水肿，这表明thimcinolne有效地将白血病细胞在视网膜中捕获，并改善了视网膜的光凝后