Synaptic vesicle recycling and changes in synaptic efficacy

突触小泡回收和突触功效的变化

• 批准号: 16300130
• 负责人: KUROMI Hiroshi
• 金额: $ 9.54万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16300130/
• 关键词: synaptic vesicle; recycling; endocytosis; drosophila; exocytosis; channel; active-zone; La3+; ノンアクチブゾーン; シナプス伝達; 高頻度刺激; Ca2+チャネル; PLTXII; タンパクリン酸化

We wish to clarify mechanisms of endocytosis of synaptic vesicles and roles of endocytosis in synaptic transmission at Drosophila Neuromuscular junction.1. It has been believed that exocytosis and endocytosis of synaptic vesicles are controlled by the same Ca2+ influx, because endocytosis always follows exocytosis and both process require external Ca2+. We found that La3+ and fulunarizine, Ca2+ channel blocker, depressed endocytosis without affecting exocytosis, while PLTXII, Ca2+ channel blocker from spider toxin, depressed predominantly exocytosis. These results suggest that Ca2+ influx through distinct routes controls exocytosis and endocytosis.2. Using Ca2+ channel blockers, we revealed two distinct endocytosis; active-zone endocytosis and non-active-zone endocytosis. Active-zone endocytosis is clathrin-independent and operate early phase of high activity, whereas non-active-zone endocytosis is clathrin-dependent and works in late phase of high activity. These results suggest that synaptic transmission at high activity is maintained coordinately by two types of endocytosis.

我们希望阐明突触小泡内吞作用的机制以及内吞作用在果蝇神经肌肉接头突触传递中的作用。 1．人们认为突触小泡的胞吐作用和内吞作用是由相同的Ca2+流入控制的，因为内吞作用总是在胞吐作用之后进行，并且这两个过程都需要外部Ca2+。我们发现La3+和氟桂利嗪（Ca2+通道阻滞剂）抑制内吞作用而不影响胞吐作用，而PLTXII（来自蜘蛛毒素的Ca2+通道阻滞剂）主要抑制胞吐作用。这些结果表明Ca2+通过不同途径流入控制胞吐作用和内吞作用。2．使用 Ca2+ 通道阻滞剂，我们揭示了两种不同的内吞作用；活性区内吞作用和非活性区内吞作用。活性区内吞作用不依赖于网格蛋白，并在高活性的早期阶段进行，而非活性区内吞作用则依赖于网格蛋白，并在高活性的后期进行。这些结果表明，高活性的突触传递是通过两种类型的内吞作用协调维持的。

项目成果

Repetitive exposures to nicotin induce a hyperresponsiveness via the cAMP/PKA/CREB signal pathway in Drosophila.

果蝇重复接触尼古丁会通过 cAMP/PKA/CREB ​​信号通路诱导高反应性。

• 发表时间: 2004
• 期刊: J.Neurobiol. 60
• 作者: Hou;J.
• 通讯作者: J.

Experience-dependent formation and recruitment of large vesicles from reserve pool.

依赖于经验的形成和从储备库中招募大囊泡。

• 发表时间: 2006
• 期刊: Neuron 50
• 作者: Steinert JR;Kuromi H;Hellwig A;Knirr M;Wyatt AW;Kidokoro Y;Schuster CM
• 通讯作者: Schuster CM

Analysis of conditional paralytic mutants in Drosophila SERCA reveals novel mechanisms for regulating membrane excitability

• 发表时间: 2004
• 作者: S. Sanyal;C. Consoulas;H. Kuromi;A. Basole;L. Mukai;Y. Kidokoro;S. K.;Krishnan;M. Ramaswami

Ca2+ influx through distinct routs controls exocytosis and endocytosis at Drospophila presynaptic terminals.

Ca2 通过不同途径流入控制果蝇突触前末端的胞吐作用和内吞作用。

• 发表时间: 2004
• 期刊: Neuron 41
• 作者: Kuromi;H.
• 通讯作者: H.

Ca2+ influx through distinct routs controls exocytosis and endocytosis at Droshophila presynantic terminals

Ca2 通过不同途径流入控制果蝇突触前末端的胞吐作用和内吞作用

• 发表时间: 2004
• 期刊: Neuron 41
• 作者: Kuromi;H.;Honda;A.;Kidokoro;Y.
• 通讯作者: Y.