Study of mechanism for synaptic vesicle recycling

突触小泡回收机制研究

基本信息

批准号：
18300131
负责人：
KUROMI Hiroshi
金额：
$ 10.12万
依托单位：
Gunma University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18300131/
关键词：
synaptic vesicles drosonhila endocytosis exocytosis recycle synaptic transmission active-zone non-active zone 神経終末 active-zone non-actve zone チュブリン synapse synaptic vesicles endocytosis La3+synaptic transmission

项目摘要

We have revealed four main findings on synaptic vesicle (SV) recycling in the period of April first, 2005 and March 31st, 2008. 1). There are two distinct pathways for endocytosis of SVs at the nerve terminals of Drosophila larvae and synaptic transmission at high frequency is maintained for a long time by two types of endocytosis which work coordinately. 2). Two types of endocytosis occurs at the active-zone and non-active zone at presynaptic nerve terminals. Two pathways are initiated by Ca2+ influx through two types of Cmaina2+ channels. Ca2+ channels which are encoded by straightjacket gene are identified as the channels which control active-zone endocytosis. 3). There has been no report to show the existence of tuburin at the nerve terminals. We produced transgenic flies which expressed GF-tubulin. GFP-tubulin changed their distribution in nerve terminals in response to nerve stimulation. Taxol, a tabilizer of tubulin, changed extents of endocytosis and traffic of SVs in nerve terminals. 4). After high activity of individual flie, synaptic transmission was enhanced by two steps. In the early phase, the quantum size increased and then the quantal contents increased.

我们已经揭示了2005年4月第一期和2008年3月31日的4月1日，关于突触囊泡（SV）回收的四个主要发现。1）。在果蝇幼虫的神经末端有两种不同的途径，可以通过两种类型的内吞作用来维持果蝇幼虫的神经末端和高频的突触传播。 2）。两种类型的内吞作用发生在突触前神经末端的活性区和非活动区域。 Ca2+流入通过两种类型的CMAINA2+通道开始了两种途径。由直夹克基因编码的Ca2+通道被鉴定为控制活跃区内吞作用的通道。 3）。没有报道显示神经终端的丘布林存在。我们产生了表达GF-管蛋白的转基因蝇。 GFP微管蛋白因响应神经刺激而改变了其在神经末端的分布。紫杉醇是微管蛋白的Tabilizer，改变了神经终端中SVS的内吞作用和流量的范围。 4）。在单个FLIE活动中高活动后，通过两个步骤增强了突触传播。在早期，量子大小增加，然后数量含量增加。