Plastic changes associated with spontaneous motor activity at neuromuscular junction.

与神经肌肉接头自发运动活动相关的塑性变化。

• 批准号: 19300140
• 负责人: KUROMI Hiroshi
• 金额: $ 11.9万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19300140/
• 关键词: ニューロン・シナプス機能; ショウジョウバエ; 運動神経一筋接合部; シナプス伝達; シナプス小胞; シナプス可塑性; 小胞エンドサイトシス; 小胞リサイクル; リザーブプール; Ca2+チャネル; 神経筋シナプス; Ca^<2+>イメージング; Ca^<2+>チャネル

The present study revealed that (1) there were multiple Ca^<2+> channels at motor nerve terminals of Drosophila larvae. (2) After exocytosis of synaptic vesicles at nerve terminals, endocytosis, the first step of vesicle recycling, occurred at active zone and non-active zone linked to each specific CaCa^<2+> channels. (3) Both endocytosis contribute coordinately to maintain synaptic transmission, depending on degree of motor nerve activity. (4) In addition, changes in tubulin states, monomer or dimmer and their distribution, were associated with dynamics of synaptic vesicles. These results suggest that changes in synaptic vesicle traffic underlines plastic changes in synaptic transmission after strong activity of motor nerve.

本研究表明：(1)果蝇幼虫运动神经末梢存在多个Ca^2+通道。 (2)神经末梢突触小泡胞吐后，小泡回收的第一步即内吞作用发生在与各特定CaCa^2+通道相连的活性区和非活性区。 (3) 两种内吞作用根据运动神经活动的程度协调地维持突触传递。 (4)此外，微管蛋白状态、单体或二聚体及其分布的变化与突触小泡的动态相关。这些结果表明，突触小泡交通的变化强调了运动神经强烈活动后突触传递的可塑性变化。