Specification of the factor in a liver cell required for multiplication of hepatitis C virus

丙型肝炎病毒增殖所需的肝细胞因子的规格

• 批准号: 13670521
• 负责人: CHAYAMA Kazuaki
• 金额: $ 1.92万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670521/
• 关键词: hepatitis C virus; chronic hepatitis; acute hepatitis; full length clone; replicon; HCV infectiousclone; IFN-β; RNA interference; IRFファミリー; polymerase; helicase; protease; polymerase chain reaction

Hepatitis C virus infection is a serious health problem worldwide. As the hepatitis C virus infects only humans and chimpanzees, establishment of reliable culture system is necessary to study this important pathogen. We cloned a full length genotype 1b cDNA clone of the hepatitis C virus from a patient who developed acute hepatitis C. This cDNA clone was used to construct several replicon clones and infectious clones. These constructs should be useful to study the hepatitis C virus.

丙型肝炎病毒感染是世界范围内的一个严重的健康问题。由于丙型肝炎病毒仅感染人类和黑猩猩，因此有必要建立可靠的培养系统来研究这种重要的病原体。我们从患有急性丙型肝炎的患者身上克隆了丙型肝炎病毒的全长基因型 1b cDNA 克隆。该 cDNA 克隆用于构建多个复制子克隆和感染性克隆。这些构建体应该有助于研究丙型肝炎病毒。

项目成果

K.Tsuboi, S.Tazuma, H.Ochi, Chayama K.: "Hydrophilic bile salts have a cyclosporine A-induce cholestasis through enhanced canalicular membrane fluidity and transporter activity"Hepatol Res. 25(1). 38-47 (2003)

K.Tsuboi、S.Tazuma、H.Ochi、Chayama K.：“亲水性胆汁盐具有环孢菌素 A-通过增强小管膜流动性和转运蛋白活性诱导胆汁淤积”Hepatol Res。

W.Ohishi, M.Kitamoto, Chayama K.ほか7名: "Impact of aging on the development of hepatocellular carcinoma in patients with hepatitis C virus infection in japan."Scand J Gastroenterol. 38,8. 894-900 (2003)

W.Ohishi、M.Kitamoto、Chayama K. 和其他 7 人：“日本丙型肝炎病毒感染患者中衰老对肝细胞癌发展的影响”，Scand J Gastroenterol 38,8 (2003)。

Waka Ohishi, K.Chayama: "Rare Quasispecies in the YMDD motif of Hepatitis B Virus Detected by Polymerase Chain Reaction with Peptitde Nucleic Acid Clamping"Intervirology. 46・6. 355-361 (2003)

Waka Ohishi，K.Chayama：“通过肽核酸钳位的聚合酶链反应检测乙型肝炎病毒 YMDD 基序中的稀有准种”Intervirology 46・6 (2003)。

S.Tazuma, T.Nishioka, Chayama K et al.: "Impaired gallbladder mucosal function in aged gallstone patients suppresses gallstone recurrence after successful extracorporeal shockwave lithotripsy"J Gastroenterol Hepatol. 18(2). 157-161 (2003)

S.Tazuma、T.Nishioka、Chayama K 等人：“老年胆结石患者胆囊粘膜功能受损可抑制体外冲击波碎石术成功后胆结石复发”J Gastroenterol Hepatol。

W.Ohishi, M.Kitamoto, Chayama K.et al.: "Impact of aging on the development of hepatocellular carcinoma in patients with hepatitis C virus infection in japan"Scand J Gastroenterol. 38(8). 894-900 (2003)

W.Ohishi、M.Kitamoto、Chayama K.等人：“日本丙型肝炎病毒感染患者中衰老对肝细胞癌发展的影响”Scand J Gastroenterol。