Identification of SNPs on mature micro RNA to analyze origin of miRNA

鉴定成熟 micro RNA 上的 SNP 以分析 miRNA 的起源

• 批准号: 25670369
• 负责人: CHAYAMA Kazuaki
• 金额: $ 2.41万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2013
• 资助国家: 日本
• 起止时间: 2013-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-25670369/
• 关键词: 肝臓学; SNP; digital PCR; microRNA; transplantation

Recent research made it clear that there are certain amount of circulating miRNA in serum, which present in serum associated with proteins such as AGO1 and AGO2. Such AGO-protein bound miRNA is quite stable and detectable by polymerase chain reaction after extraction from the serum samples and reverse transcription. We attempted to analyze organs which secrete each miRNA by analyzing circulating miRNA that is present in serum samples. To perform this analysis we tried to identify SNPs that is present in miRNA. We searched for such SNP in entire human genome analyzing human genome database and identified several candidate SNPs. Although we attempted to detect such miRNA with digital PCR, detection of small amount of miRNA was quite difficult. Further study is needed to detect such small amount of miRNA in serum samples.

最近的研究表明，血清中存在一定量的循环miRNA，其存在于血清中与AGO1和AGO2等蛋白相关。这种 AGO 蛋白结合的 miRNA 相当稳定，从血清样品中提取并逆转录后可通过聚合酶链反应检测到。我们试图通过分析血清样本中存在的循环 miRNA 来分析分泌每种 miRNA 的器官。为了进行此分析，我们尝试识别 miRNA 中存在的 SNP。我们在整个人类基因组分析人类基因组数据库中搜索了这样的SNP，并确定了几个候选SNP。尽管我们尝试用数字PCR检测此类miRNA，但检测少量miRNA是相当困难的。需要进一步研究来检测血清样本中如此少量的 miRNA。

项目成果

Letter : Pitavastatin supplementation of PEG-IFN/ribavirin improves sustained virological response against HCV

信件：补充 PEG-IFN/利巴韦林的匹伐他汀可改善针对 HCV 的持续病毒学反应

• DOI: 10.1111/apt.12605
• 发表时间: 2014
• 期刊: Aliment Pharmacol Ther
• 作者: Yokoyama S;Kawakami Y;and Chayama K
• 通讯作者: and Chayama K

Thromboxane A2 Synthase Inhibitors Prevent Production of Infectious Hepatitis C Virus in Mice with Humanized Livers.

血栓素 A2 合酶抑制剂可防止具有人源化肝脏的小鼠产生传染性丙型肝炎病毒。

• DOI: 10.1053/j.gastro.2013.05.014
• 发表时间: 2013
• 期刊: Gastroenterology
• 影响因子: 29.4
• 作者: Abe Y;Aly HH;Hiraga N;Imamura M;Wakita T;Shimotohno K;Chavama K;Hijikata M.
• 通讯作者: Hijikata M.

Effect of vitamin D supplementation on pegylated interferon/ribavirin therapy for chronic hepatitis C genotype 1b: a randomized controlled trial

• DOI: 10.1111/jvh.12146
• 发表时间: 2014-05
• 期刊: Journal of Viral Hepatitis
• 影响因子: 2.5
• 作者: S. Yokoyama;S. Takahashi;S. Takahashi;Y. Kawakami;Y. Kawakami;C. N. Hayes;Hirotaka Kohno;K. Tsuji;Y. Aisaka;S. Kira;K. Yamashina;M. Nonaka;T. Moriya;M. Kitamoto;S. Aimitsu;T. Nakanishi;H. Kawakami;K. Chayama;K. Chayama

消化器内科

胃肠病学

• 发表时间: 2014
• 作者: 五十嵐央祥;能正勝彦;篠村恭久ら
• 通讯作者: 篠村恭久ら

Treatment of Chronic Hepatitis C with First Generation Protease Inhibitors in Asian Population:Telaprevir-Based Triple Therapy in Japanese Population

第一代蛋白酶抑制剂治疗亚洲人群慢性丙型肝炎：日本人群基于特拉匹韦的三联疗法

• 发表时间: 2013
• 作者: Chayama K ;Hayes CN;Ohishi W,Kawakami Y;茶山一彰;茶山一彰;茶山一彰;茶山一彰;茶山一彰;茶山一彰;茶山一彰;茶山一彰;茶山一彰;茶山一彰;茶山一彰;茶山一彰;茶山一彰;茶山一彰;茶山一彰;茶山一彰;茶山一彰
• 通讯作者: 茶山一彰