Immunohistochemical Study on the Occurrence, Morphology and Development of the Gastrointestinal Brush Cells of the Rat

大鼠胃肠刷细胞发生、形态及发育的免疫组织化学研究

• 批准号: 01570006
• 负责人: ISEKI Shoichi
• 金额: $ 1.34万
• 依托单位: School of Medicine, Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570006/
• 关键词: Brush cell; Common bile duct; Fatty acid binding protein; Immunocytochemistry; Rat; Scanning electron microscopy; Stomach; 胆管

The brush cell, also referred to as the tuft cell, is a rare cell type known to occur in the endoderm-derived epithelia of many mammalian species. In the present study, we found that an antibody against Liver-type Fatty Acid-Binding Protein (L-FABP) specifically immunostained the gastrointestinal brush cells of the rat, and utilized this immunoreactivity as a specific marker of the brush cell to elucidate its previously unclarified aspects.The largest populations of brush cells were found in the stomach and common bile duct. In the stomach, brush cells were present in a group in the groove of cardiac region as well as scattered singly in the surface and foveolar epithelia of the fundic and pyloric regions, with intense immunoreactivity for L-FABP. Morphologically, it was clarified that all brush cells had a thin basal cytoplasmic process without secretory granules. In the ontogeny of rats, brush cells first occurred in the new born stomach, with immunoreactivity for L-FABP, and increas … More ed remarkably in number within the few weeks following the end of the first 2 weeks of sackling period.In the common bile duct, The brush cells as recognized by scanning electron microscopy first appeared in the epithelium at 4 weeks after birth. They showed a remarkable increase in number between 8 and 16 weeks and finally occupied about 30 % of the total epithelial cell population of common bile duct. The initial development of brush cells in the females delayed for approximately 2 weeks from that of males. On the other hand, the L-FABP-immunoreactive brush cells first appeared only after 8 weeks postpartum. They showed a gradual increase in number after 16 weeks but reached only about 25 % of the total brush cell population by the time of 40 weeks.The present study, by virtue of the specific histochemical marker, provided new findings concerning the distribution, morphology and ontogeny of the brush cell. Also, the expression of L-FABP in the brush cell may shed light on the functional aspect of this cell type in relation to the fatty acid meTabolism. Less

刷细胞，也称为簇细胞，是一种罕见的细胞类型，存在于许多哺乳动物物种的内胚层衍生上皮中。在本研究中，我们发现了一种针对肝型脂肪酸结合蛋白的抗体。 (L-FABP)对大鼠胃肠道刷细胞进行特异性免疫染色，并利用这种免疫反应性作为刷细胞的特异性标记物来阐明其先前未阐明的方面。发现了最大的刷细胞群体胃及胆总管中，贲门区沟内成群存在刷状细胞，胃底及幽门区表面及小凹上皮呈单散分布，对L-FABP具有强烈的免疫反应性。形态学上表明，所有刷状细胞均具有薄的基底胞质突起，无分泌颗粒。在大鼠个体发育过程中，刷状细胞首先出现在新生胃中，具有免疫反应性。 L-FABP，并且在前 2 周的麻袋期结束后的几周内数量显着增加。在胆总管中，通过扫描电子显微镜识别的刷状细胞首先出现在上皮细胞中，时间为 4出生后几周，它们的数量在8至16周之间显着增加，最终占胆总管上皮细胞总数的约30%。与男性相比，L-FABP 免疫反应性刷状细胞在产后 8 周后才首次出现，16 周后数量逐渐增加，但仅达到总数的 25% 左右。 40周时刷状细胞群体。本研究凭借特定的组织化学标记，提供了有关刷状细胞的分布、形态和个体发育以及L-FABP在刷状细胞中表达的新发现。可能揭示这种细胞类型与脂肪酸代谢相关的功能。

项目成果

Iseki, S., Kanda, T., Hitomi, M. and Ono, T.: "Ontogenic appearance of three fatty acid binding proteins in the rat stomach." Anat. Rec. 229. 51-60 (1991)

Iseki, S.、Kanda, T.、Hitomi, M. 和 Ono, T.：“大鼠胃中三种脂肪酸结合蛋白的个体出现。”

Iseki,S.and Kondo,H.: "Specific localization of hepatic fatty acid binding protein in the gastric brush cells of rats." Cell and Tissue Research. 257. 545-548 (1989)

Iseki,S. 和 Kondo,H.：“大鼠胃刷细胞中肝脂肪酸结合蛋白的特异性定位。”

Iseki, S. and Kondo, H: "An immunocytochemical study on the occurrence of liver fatty-acid-binding protein in the digestive organs of rats : specific localization in the D cells and brush cells." Acta Anat. 138. 15-23 (1990)

Iseki, S. 和 Kondo, H：“对大鼠消化器官中肝脏脂肪酸结合蛋白发生的免疫细胞化学研究：D 细胞和刷状细胞中的特异性定位。”

Iseki,S.,Kondo,H.: "An immunocytochemical study on the occurrence of liver fatty-acid-binding protein in the digestive organs of rats:specific localization in the D cells and brush cells" Acta Anatomica. (1990)

Iseki,S.,Kondo,H.：“大鼠消化器官中肝脏脂肪酸结合蛋白发生的免疫细胞化学研究：D 细胞和刷状细胞中的特异性定位”《解剖学报》。

Directions in bivalve feeding

双壳类的饲养方法

• DOI: 10.2312/conf/eg2013/stars/117-136
• 发表时间: 2024-09-14
• 期刊: 2013 IEEE Symposium on Large-Scale Data Analysis and Visualization (LDAV)
• 作者: P. Beninger;Beninger Pg;Pascoe Pl;Parry He;Hawkins Ajs;A. Robson;G. Thomas;R. Wilson;M. Ll;V. Bricelj;C. Ouellette;C. Leger;Bates Ss;A. Persson;Smith Bc;G. Yahel;D. Marie;S. Eckstein;A. Genin;E. Navarro;S. Méndez;Urrutia Mb;Lonsdale Dj;Cerrato Rm;R. Holl;L. Holt;Schaffner Ra;J. Pan;N. Tezuka;E. Ichisaki;M. Kanematsu;K. Iseki
• 通讯作者: K. Iseki