Expression and localization of vascular endothelial growth factor in the endocrine system

血管内皮生长因子在内分泌系统中的表达和定位

基本信息

批准号：
09670006
负责人：
ISEKI Shoichi
金额：
$ 2.05万
依托单位：
Kanazawa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670006/
关键词：
vascular endothel-ial growth factor VEGF endocrine cells mast cells immunohistochemistry rat 内分泌腺

项目摘要

Vascular endothelial growth factor (VIEGE) is a potent angiogenic mitogen and also increases vascular permeability. Immunohistochemical localization of VEGF in the entire body of healthy adult rats was investigated at light and electron microscopic levels using a specific antibody. Intense to moderate immunoreactivities for VEGF were detected in certain endocrine cell types, namely, the parafollicular cell of thyroid gland, B cell of endocrine pancreas, N cell of adrenal medulla and a minority of the TSH cell of anterior pituitary gland. At the ultrastructural level, VEGF immunoreactivity was localized exclusively in the secretory granules of all immunopositive endocrine ells. These results provided immunohistochemical evidence for the occurrence of VIEGF in subsets of endocrine cells of normal adult rats, suggesting that these secretory cells regulate local vascular permeability through a paracrine action of VEGF (Reference 1).The results also showed solitary cells with strong VEGF im … More munoreactivity scattered in the epithelium of the respiratory tract as well as in the lamina propria and epithelium of the small and large intestines. By ultrastructural features of their large cytoplasmic granules, these VEGF-positive cells were identified as globule leukocytes (GL), also called mucosal mast cells (MMC). The immunoreactivity was localized exclusively in the cytoplasmic granules of GIIMMC.When examined in serial sections, GL/MMC in the respiratory and digestive tracts showed only weak reactivity to anti-histamine antibody. In contrast, connective tissue mast cells (CTMC), which were located in the submucosa of digestive tract and in the connective tissue of respitratory tract and a variety of other organs, were intensely immunopositive for histamine, whereas they showed no reactivity for VBGF.The specific appearance of VEGF immunoreactivity in GL/MMC suggested that this cell type is involved in the paracrine regulation of the permeability of nearl5y microvessels, and that VEGE can be used as a histochemical marker to distinguish GL/MMC from CTMC(Reference2). Less

血管内皮生长因子 (VIEGE) 是一种有效的血管生成促细胞分裂剂，还可增加健康成年大鼠全身血管通透性，使用特定抗体在光镜和电子显微镜水平上研究了 VEGF 的强至中度免疫反应性。在某些内分泌细胞类型中检测到，即甲状腺滤泡旁细胞、内分泌胰腺的 B 细胞、胰腺的 N 细胞肾上腺髓质和垂体前叶的少数 TSH 细胞在超微结构水平上，VEGF 免疫反应性仅位于所有免疫阳性内分泌细胞的分泌颗粒中。这些结果为内分泌细胞亚群中发生 VIEGF 提供了免疫组织化学证据。正常成年大鼠，表明这些分泌细胞通过 VEGF 的旁分泌作用调节局部血管通透性（参考文献 1）。结果还显示出具有强 VEGF 免疫反应性的孤立细胞分散在呼吸道上皮以及小肠和大肠的固有层和上皮中，通过其大细胞质颗粒的超微结构特征，鉴定出这些 VEGF 阳性细胞。球白细胞 (GL)，也称为专门的粘膜肥大细胞 (MMC)，免疫反应性位于细胞质颗粒中。 GIIMMC。在连续切片中检查时，呼吸道和消化道中的 GL/MMC 仅显示出抗组胺抗体的弱反应性，相反，位于消化道粘膜下层和消化道中的结缔组织肥大细胞 (CTMC)。呼吸道和多种其他器官的结缔组织对组胺呈强烈免疫阳性，而对 VBGF 无反应性。 VEGF 免疫反应性的具体表现GL/MMC提示该细胞类型参与近15年微血管通透性的旁分泌调节，VEGE可作为区分GL/MMC与CTMC的组织化学标志物(参考文献2)。