Basic study on the development of "Bio-Defence Foods" by DNA motifs from dairy lactic acid bacteria

利用乳制品乳酸菌DNA基序开发“生物防御食品”的基础研究

基本信息

批准号：
13660265
负责人：
KITAZAWA Haruki
金额：
$ 2.56万
依托单位：
Tohoku University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13660265/
关键词：
Probiotics L.bulgaricus L.gasseri DNA cytokine Peyer's patch TH1 Lactic acid bacteria L.bulgaricu 樹状細胞走化性因子

项目摘要

Fermented milks and their related dairy lactic acid bacterla have long been thought to be of value for health maintenance. Recently, corroborative experiments on the physiological functions of dairy lactic acid bacteria (LAB), especially the probiotic LAB, and their derivatives have been carried out. A great interest has been focused on biological functions such as immunomodulations. In our recent study, we have been particularly focusing on the DNA from LAB as one of the immunomoduratory factors in the LAB. The present study was conducted to elucidate the cytokine induction by the DNA in the gut. After stimulation of swine peyer's patch and mesenteric lymph node wlth DNA motifs from the LAB, induction of cytokine mRNA was estimated by real time PCR method. In addition, a novel function of L gasseri was investigated. The DNA motifs augmented induction of TH1 type cytokine (IL-6, IL-12, IFNy) mRNA in peyer's patches. The macrophage clemotaxis induced by L gasseri JCM1131^T culture supematants, which the chemotactic factor was designated as "Gasserokine", was discovered to be a new biological function exerted by probiotic lactic acid bacteria. Therefore, the activity is expected to be used for one of the functional parameters in the immunomodulating properties of probiotic lactic acid bacteria. This study demonstrated that immunostimulatory DNA motif from the LAB exerted the cytokine induction in the gut and proposed that the LAB is a good candidate of a starter culture for the production of new functional foods, "Bio-Defense Foods".

长期以来人们认为发酵奶及其相关乳制品乳酸细菌对健康维持具有价值。最近，已经进行了乳酸菌（LAB）的生理功能的佐证实验，尤其是益生菌实验室及其衍生物。极大的兴趣集中在生物学功能（例如免疫调节）上。在我们最近的研究中，我们特别关注实验室的DNA，是实验室中的免疫修饰因素之一。进行了本研究以阐明肠道中DNA的细胞因子诱导。在实验室刺激猪Peyer斑块和肠系膜淋巴结WLTH DNA基序后，通过实时PCR方法估算了细胞因子mRNA的诱导。此外，研究了L Gasseri的新功能。 DNA序列增强了佩耶斑块中Th1型细胞因子（IL-6，IL-12，IFNY）mRNA的诱导。 L Gasseri JCM1131^t培养型伴侣诱导的巨噬细胞轻曲霉，将其趋化因子指定为“ Gasserokine”，被认为是由果物乳酸菌细菌施加的一种新的生物学功能。因此，预计该活性将用于益生菌乳酸菌细菌的免疫调节特性中的功能参数之一。这项研究表明，实验室的免疫刺激性DNA基序在肠道中施加了细胞因子诱导，并提出实验室是生产新功能性食品“生物防御食品”的启动培养物的良好候选者。