Study on anti-allergic activity of DNA from immunobiotics and its application for biodefence foods.

免疫生物DNA的抗过敏活性及其在生物防御食品中的应用研究

基本信息

批准号：
15380183
负责人：
KITAZAWA Haruki
金额：
$ 9.09万
依托单位：
Tohoku University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15380183/
关键词：
Lactic acid bacteria Probiotics Immunobiotics Toll-like receptor DNA AT-oligodeoxynucleotide Peyer's patches Mesenteric lymph nodes Immunobiotics AT oligonucleotide 腸管膜リンパ節 TLR9 トランスフェクタント

项目摘要

Recent interest has focused on the importance of intestinal immunity for the host defense, but to date, not much has been known about the underlying mechanisms. Toll-like receptor (TLR) family plays an important role in the defense through recognizing bacterial pathogen associated molecular patterns (PAMPs). In this study, the physiological function of food products has investigated the immunoregulatory effects of immunobiotic lactic acid bacteria (LAB) via TLRs. Studies in swine, which is expected as a human model, have been examined intestinal immunoregulation by the immunobiotic LAB. Our research has now demonstrated regulation of intestinal immunity mediated by TLRs in the gut. On the basis of this study, efforts have also been made to develop a molecular immunoassay system for immuobiotic LAB and find novel immunostimulatory DNA sequences from imunobiotics. In vitro and in vivo study demonstrated an anti-allergic activity of immunostimulatory DNA sequences from imunobiotics. The findings in this project may provide impetus to further delineate the activation mechanism of the innate immune response with immunostimulatory DNA. In addition, identification of immunoregulatory factors from LAB, and better understanding of intestinal immune regulation through cytokine networks hold promise in basic research of food immunology and development of "immunobiotic foods" to prevent allergic disease.

最近的兴趣集中在肠道免疫对于宿主防御的重要性，但迄今为止，人们对其潜在机制知之甚少。 Toll 样受体 (TLR) 家族通过识别细菌病原体相关分子模式 (PAMP) 在防御中发挥重要作用。在这项研究中，食品的生理功能研究了免疫乳酸菌 (LAB) 通过 TLR 的免疫调节作用。免疫生物实验室已对猪进行了研究，预计将其作为人类模型，对肠道免疫调节进行了检查。我们的研究现已证明肠道免疫的调节是由肠道中的 TLR 介导的。在此研究的基础上，我们还致力于开发免疫生化实验室的分子免疫分析系统，并从免疫生化中寻找新的免疫刺激DNA序列。体外和体内研究证明了免疫生物的免疫刺激 DNA 序列具有抗过敏活性。该项目的研究结果可能为进一步阐明免疫刺激 DNA 的先天免疫反应的激活机制提供动力。此外，从LAB中鉴定免疫调节因子，以及通过细胞因子网络更好地了解肠道免疫调节，为食品免疫学的基础研究和开发预防过敏性疾病的“免疫生物食品”带来了希望。