Analysis of gene expression concerned with PDL mechanical receptor in biting

咬合过程中PDL机械受体相关基因表达分析

• 批准号: 12671896
• 负责人: BABA Shunsuke
• 金额: $ 1.73万
• 依托单位: Kyoto Prefectural University of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671896/
• 关键词: cytokine; PDL; 咬合接触; 咬合挙上

Periodontal ligament (PDL) cells, which consist of osteoblasts, fibloblasts, osetoclasts, and et.al., maintain the attachment of the tooth to alveolar bone. Recently, it has been reported that PDL cells examines function and characterization in vitro. To confirm immune response for periodontal disease casing dental plaque on PDL cells, we investigated the cytokine Mrna expression and production stimulating Prevotella intermedia and Streptococcus mutans. PDL cells were obtained from the middle third of the root surface of healthy human erupted maxillary third molar. The tissue minced and cultured as explants in tissue culture medium [D-MEM with D-Glucose 4500 mg/dl L-Glu NaHCO3 ] containing 10% low-endotoxin fetal calf serum (FCS), 100U penisillin, 25ug/ml streptomysin, amphotericin B as FugizoneTM in 0.85% saline). Cultures were then grown in medium and cloned by limiting dilutions, I.e., the cells obtained from primary cultures. The confluent cultures were used for experimentation bet … More ween the 3th and 4th passages. Bacteria were the kind gift from department of Microbiology, Kyoto Prefectural University of Medicine. Cultures were confluent (1.0× 105cells/well). And stimulated by bacteria (1.0×107/ml) after 24 hours in a 6-well plate. No bacteria were used as control. Total RNA was extracted by ISOGENTM (NIPPON GENE). 1mg of RNA was used for Cdna synthesis with a reverse transcriptase (SuperscriptII RNAse H; GIBCO-BRL) and an oligo (dt) 16 primer. Reaction mixture was used as a template for PCR amplification was run for 35 cycles at 94℃ for 1 min, 60℃ for 1 min, and 72℃ for 1 min. PCR product was analyzed by electrophoresis on a 1.5% agarose gel containing ethidium bromide, and the bands were examined under UV light. The expression of IL-1b, IL-6, IL-8, TNF-α, TGF-α, and TGF-β were detected. This study indicated expression of many inflammatory cytokines. These results suggest that there was a relationship between the cytokine expression and the inflammation of periodontal ligaments on pockets. Furthermore, we may examine immune mechanism which marginal periodontitis affect mechanical stress and Bone Morphogenetic Protein Less

牙周韧带（PDL）细胞，由骨细胞，骨质骨骼和等等组成，并保持AR骨的附着。我们在PDL细胞上研究了细胞因子mRNA表达刺激ELA中间和链球菌变异物，如在组织培养基中的组织和培养。低粘毒素胎牛血清（FCS）阴茎素，25ug/ml链球菌素B，在0.85％的盐水中作为fugizonetm，通过限制性稀释液，将其用于实验。第三次和第4段。 1mg的RNA是cDNA合成的，用逆转录酶D寡核酸（DT）16底漆。 UM溴和紫外线的表达。和口袋上的牙周韧带的炎症。

项目成果

Namba M, Yamada K, Kudeken W, Mizutani G, Tsukitani K, Mori M, Takai Y: "Phenotypic changes of calponin in salivary gland myoepithelial cells during postnatal development of rats"Acta Histochem, Cytochem. 33(2). 73-79 (2000)

Namba M、Yamada K、Kudeken W、Mizutani G、Tsukitani K、Mori M、Takai Y：“大鼠出生后发育过程中唾液腺肌上皮细胞中钙调蛋白的表型变化”Acta Histochem，Cytochem。

Masataka Itoigawa, Chihiro Ito, Hugh T.W.Tan, Masato Okuda, Harukuni Tokuda, Hoyoku Nishino, Hiroshi Furukawa: "Cancer chemoprevetive activity of naphthoquinones and their analogs From Avicennia plants"Cancer Letters. 174・2. 135-139 (2001)

Masataka Itoikawa、Chihiro Ito、Hugh T.W.Tan、Masato Okuda、Harukuni Tokuda、Hoyoku Nishino、Hiroshi Furukawa：“来自白骨壤植物的萘醌及其类似物的癌症化学预防活性”癌症快报 174・2。

Namba M, Yamada K, Kudeken W, Mizutani G, Tsukikani K, Mori M, Takai Y: "Phenotypic changes of calponin in salivary gland myoepithelial cells during postnatal development of rats"Acta Histochem. Cytochem.. 33・2. 73-79 (2000)

Namba M、Yamada K、Kudeken W、Mizutani G、Tsukikani K、Mori M、Takai Y：“大鼠出生后发育过程中唾液腺肌上皮细胞中钙调蛋白的表型变化”细胞化学学报.. 73・2。 (2000)

Yamada K, Namba M, Sumitomo S, Mori M, Tsukitani K, Shrestha P, Takai Y: "Heterogeneity of expression of calponin and metallothionein in reactive myoepithehal/ductal basal cells of obstructive sialadenitis"Acta Histochem.Cytochem.. 33・4. 287-294 (2000)

山田K、难波M、住友S、森M、月谷K、Shrestha P、高井Y：“阻塞性唾液腺炎反应性肌上皮/导管基底细胞中钙调蛋白和金属硫蛋白表达的异质性”组织化学学报.细胞化学.. 33・4。 287-294 (2000)

Yamada K, Namba M, Sumitomo S, Mori M, Tsukitani K, Shrestha P, Takai Y: "Imaginary mastication causes primary sensorimotor cortex activation"Acta Histochem, Cytochem. 33(4). 287-294 (2000)

Yamada K、Namba M、Sumitomo S、Mori M、Tsukitani K、Shrestha P、Takai Y：“想象的咀嚼导致初级感觉运动皮层激活”Acta Histochem，Cytochem。