Development oh tissue-engineered bone with bio absovbency which holds occlusal support.

开发具有生物吸收性的组织工程骨，可提供咬合支撑。

• 批准号: 16591976
• 负责人: BABA Shunsuke
• 金额: $ 2.11万
• 依托单位: Foundation for Biomedical Research and Innovation
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16591976/
• 关键词: Regenerative medicine; scafold

In this research, it is in the development of the scaffold to which it gives priority to a dynamic function of the bone by using the cultured cell and the biological absorption scaffold technology. The scaffold uses the growth of the osteobrast cell to treat to natural teeth and implant surroundings so that it may make amends for the cell when urge function is possessed and the reasonable amount is not obtained only by the cell. However, neither a hydroxyapatite nor a calcium phosphate system material that is the scaffold marketed now are appreciable in not the one in the point of regeneration of the bone that finishes being satisfactory but osteobrast cell formation. These problems are not to be able to promote the ability of the cell growth enough by the osteobrast cell of the self-origin and combining scaffolds. In addition, it is not a biological absorption scaffold to expect to replace native bone for a certain period in the function as the scaffold. Equip absorption, the inside p … More ore must be high, and the osteobrast cell and the blood vessel must go into it easily, and the structure with the function that the load of the occlusal force is possible in the mouth become conditions of functionally evaluating the reproductive bone. There is a feature in the point to reproduce surrounding bones of natural teeth an bones in the implant surroundings by MSCs of the self-origin that is the origin of the alveolar bone, enamel, the cement Quality, the periodontal membrane, and developing the culture bone of occlusal support that can be endured by using the scaffold with a biological absorption where the optimization design was done. In this research, by designing a biological absorption scaffold, it was assumed that the structure of the scaffold material corresponding to the revitalization degree of the culture osteobrast cell was able to be optimized, and the scaffold to be able to expect the healing of wound and the bone formation promotion action in addition to the more efficient bone reproduction was developed. The cell was settled on in the type scaffold and the plan sowing was settled on including knitting Cago referring to the result of a relation between PRP and the cell, and current clinical researches. This is the cloth structure finished knitting with one PLA fiber as a scaffold for the tissue regeneration. This scaffold has the cave internally in the Cago shape. It is because of raising the implantation rate by putting the cell in here. The one that has been put to practical use now forms the cell, and this scaffold can be cut into a necessary size by sowing internally in the reason to have the opening at the medical practice, and when operating, can be formed according to the coloboma part though is difficult sowing in the scaffold. Moreover, it is useful in the point that is the structure to which it can circulate around the body fluid by the interface in the scaffold and the coloboma part. Not only the bond efficiency of the cell to sow in the scaffold since the culture cell condition was examined to measure the optimization of the cell to sow here but also the mechanics characteristic of the scaffold having to take part became clear. In addition, there was a problem in bend strength was supplemented from the necessity for improving operativeness to surroundings of the PLA fiber with the binder of PCL. Moreover, the MSCs was bonded to squirrel-cage scaforld, and it cultured it. The biodegradation speed of the scaffold manufactured from PLA is slow as a result, externals remained being maintained for a long term of six months or more as for the scaffold though the cell reproduction and the differentiation induction to the osseous system were possible. The adjustment of the absorption degradation rate of the scaffold and the bone regeneration speed of the culture osteoblast became an important problem from these. When having experimented on the animal to confirm in Vivo of the PLA fiber, the reproduction of the bone would be confirmed in 12 weeks and the absorption of the scaffold was confirmed for that. An excellent result was obtained compared with the transplant only of the cell. Less

在这项研究中，它在将培养的培养物的动态功能的脚手架开发为生物学吸收脚手架技术中。归因于thend furge函数的修改，合理的数量仅由ll造成。骨化细胞形成。 de P…更多的矿石必须很高，而骨but骨和血管必须容易进入它，并且在功能性的骨骼的嘴巴条件下，具有咬合力的载荷的结构可能是可能的。自然牙齿周围的骨骼在植入物周围的骨骼中，牙槽骨骼的骨骼，水泥质量，牙周膜这项研究是通过设计脚手架的，假定脚手架材料的结构相关的结构与培养骨包骨细胞的斜体化度相关，并且能够优化脚手架，并且能够在伤口的愈合和骨形成动作中的脚手架。 d的骨骼繁殖是d的。在这里。 coloboma部分不仅是在脚手架上播种的细胞，以诱惑细胞的优化。从您的必要性提高PLA纤维的操作性，再到P​​CL的培养。细胞细胞系统可能会调整脚手架的吸收率和培养成骨细胞的骨骼再生速度。与细胞的移植相比，获得了出色的结果

项目成果

Osteogenic potential of injectable tissue-engineered bone : a comparison among autogenous bone, bone substitute (Bio-Oss(^【○!R】)), platelet-rich plasma(PRP), and tissue-engineered bone with respect to their mechanical properties and histological findings

可注射组织工程骨的成骨潜力：自体骨、骨替代物（Bio-Oss（^【○！R】））、富血小板血浆（PRP）和组织工程骨的机械性能比较和组织学发现

• 发表时间: 2005
• 期刊: Journal of Biomedical Materials Research 73A
• 作者: Ito;K.;Yamada;Y.;Nagasaka;T.;Baba;S.;Ueda,M.
• 通讯作者: Ueda,M.

Differential Regulation by IL-10 and EGF of Expression Three Different Hyaluronan Synthases in Oral Mucosal of Epithelial Cells and Fibroblasts and Dermal Fibroblasts : Quantitative Analysis Using Real-Time RT-PCR.

IL-10 和 EGF 对上皮细胞、成纤维细胞和真皮成纤维细胞口腔粘膜中三种不同乙酰透明质酸合成酶表达的差异调节：使用实时 RT-PCR 进行定量分析。

• 发表时间: 2004
• 期刊: J Invest Dermatol 122
• 作者: Yoichi Yamada;Naoki Itano;Ken-Ichiro Hata;Minoru Ueda;Koji Kimata
• 通讯作者: Koji Kimata

Differential Regulation by IL-1β and EGF of Expression of Three Different Hyaluronan Synthases in Oral Mucosal Epithelial Cells and Fibroblasts and Dermal Fibroblasts : Quantitative Analysis Using Real-Time RT-PCR.

IL-1β 和 EGF 对口腔粘膜上皮细胞、成纤维细胞和真皮成纤维细胞中三种不同透明质酸合成酶表达的差异调节：使用实时 RT-PCR 进行定量分析。

• 发表时间: 2004
• 期刊: J Invest Dermatol 122
• 作者: Yoichi Yamada;Naoki Itano;Ken-Ichiro Hata;Minoru Ueda;Koji Kimata
• 通讯作者: Koji Kimata

生分解性樹脂複合材料により構成される組織再生用足場(スカフォールド)

可生物降解树脂复合材料组织再生支架

• 发表时间: 2004

Osteogenic potential of injectable tissue-engineered bone : a comparison among autogenous bone, bone substitute (Bio-Oss(^*)), platelet-rich plasma (PRP), and tissue-engineered bone with respect to their mechanical properties and histological findings

可注射组织工程骨的成骨潜力：自体骨、骨替代物（Bio-Oss(^*)）、富血小板血浆（PRP）和组织工程骨的机械性能和组织学结果的比较

• 发表时间: 2005
• 期刊: Journal of Biomedical Materials Research - Part A. 73
• 作者: Ito;K.;Yamada;Y.;Nagasaka;T.;Baba;S.;Ueda;M.
• 通讯作者: M.