Central mechanism of domato-parasympathetic reflex vasodilatation

结构域-副交感神经反射性血管舒张的中枢机制

基本信息

批准号：
12671797
负责人：
IZUMI Hiroshi
金额：
$ 1.92万
依托单位：
TOHOKU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

项目摘要

We measured the changes in lower lip blood flow and systemic arterial blood pressure (SABP) evoked by lingual nerve (LN) or trigeminal spinal nucleus (Vsp) stimulation to gain an insight into the brainstem integration of sympathetic and parasympathetic responses to nociceptive stimulation. We used artificially ventilated, cervically vago-sympathectomized cats deeply anesthetized with a-chloralose and urethane. A lip blood flow (LBF) increase occurred in an intensity- and frequency-dependent manner following electrical stimulation of Vsp or LN regardless of whether SABP increased or decreased. In contrast, there was no apparent optimal frequency for the changes in SABP elicited by electrical stimulation of Vsp or LN. No relationship was found between the amplitude of the LBF increase and that of the SABP change. Microinjection of lidocaine or kainic acid into the Vsp evoked, respectively, reversible and irreversible inhibition of the LBF increase and SABP change evoked by LN stimulation … More . When microinjected unilaterally directly into the ipsilateral Vsp, the GABA agonist muscimol abolished the LN-evoked increase in lip blood flow and changes in SABP without changing basal SABP, suggesting the presence in the Vsp of a GABAergic system modulating both the parasympathetically mediated LBF increase and the sympathetically mediated SABP change. Lidocaine microinjection into the salivatory nucleus caused a significant attenuation of the LN-induced blood flow increase, but had no effect on the LN-induced SABP change. Thus, the neural pathway mediating the LN-induced LBF increase seems to be simple, requiring a minimum of four neurons : trigeminal afferent - Vsp parasympathetic pre-ganglionic neurons with cell body located in the inferior salivatory nucleus - otic postganglionic neuron. On the other hand, the pathway underlying the evoked SABP changes, presumably mediated via altered sympathetic activity, seems to be more complicated and could be affected by more numerous factors. Less

我们测量了通过舌神经（LN）或三叉神经脊柱核（VSP）模拟引起的下唇血流和全身性艺术血压（SABP）的变化，以深入了解脑干的交感神经和副副作用反应对伤害感住刺激的脑干。我们使用了人工通风的，可颈交感神经切除的猫，深深地用a-氯酸盐和氨基甲酸酯麻醉。在VSP或LN电气模拟后，无论SABP是否增加还是减少，唇血（LBF）的增加以强度和频率依赖性方式发生。相反，通过VSP或LN的电气模拟，SABP优雅的变化没有明显的最佳频率。 LBF增加的放大器与SABP变化的放大器之间没有发现任何关系。将利多卡因或海藻酸显微注射到诱发的VSP中，可逆性和不可逆转的LBF抑制作用和LN刺激引起的SABP变化……更多。当显微注射直接直接进入同侧VSP时，GABA激动剂Muscimol废除了LN诱发的唇血流动的增加和SABP的变化而没有改变基本的SABP，这表明在Gabaergic系统的VSP中的存在和副介导的LBF均具有同在的s s s s sabpemets s sabpppppppicppeppicppicts s sabppppicppicppicppicpect。 Lidocaine对唾液核的微注射会导致LN诱导的血流增加显着衰减，但对LN诱导的SABP变化没有影响。因此，介导LN诱导的LBF增加的神经元途径似乎很简单，至少需要四个神经元：三叉神经传入 - VSP副交感神经前神经元，其细胞体位于下唾液核心 - 毒性核心 - 眼动后神经发电后神经元。另一方面，引起的SABP变化的途径，大概是通过改变的交感神经活动介导的，似乎更复杂，可能受到更多因素的影响。较少的