Establishment of bioartificial pancreas using pancreatic stem all for severe diabetes mellitus

利用胰干细胞建立生物人工胰腺治疗重症糖尿病

• 批准号: 12671153
• 负责人: MIYAMOTO Masaaki
• 金额: $ 2.5万
• 依托单位: NIPPON MEDICAL SCHOOL (2002)Kyoto University (2000-2001)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671153/
• 关键词: islets; isolation procedure; cry a preservation of islets; islet transplantation; two-step isolation procedure of islets; islets; 膵島凍結保有法; バイオ人工膵; 異種移植; ブタ膵島幹細胞; 膵島皮下移植; 膵島筋肉内移植; 異種膵島移植; 膵幹細胞; 細胞移植; 糖尿病; PGP9.5

(1) Development of isolation procedure of pancreatic islets for large animals. We developed 2-step automated isolation procedure of pancreatic islets for miniature swine and dogs.This procedure is possible to use human pancreatic islets for human islets transplantation.(2) Development of automated cry a preservation procedure for pancreatic islets. We developed cry a preservation procedure employing a freezer bag for pancreatic islets using a newly developed cry a protectant.

（1）开发大型动物胰岛的隔离程序。我们开发了用于微型猪和狗的胰岛的2步自动隔离程序。该程序可以将人类胰岛用于人类胰岛移植。（2）自动化的哭泣程序开发了胰岛的保存程序。我们使用新开发的Cry A保护剂采用冰柜袋，采用冰柜袋进行哭泣程序。

项目成果

Masaaki MIYAMOTO et al.: "Cell Therapy for Diabetes Mellitus : Present Status & future Prospects"Human cell. (in press).

Masaaki MIYAMOTO 等人：“糖尿病细胞疗法：现状

Masaaki Miyamoto: "Improvement of modified Two-layer preservation method (PEC/Kyoto solution) in inlet isolation from breeder pigs"Transplantation Proceedings. 32. 1660-1661 (2000)

Masaaki Miyamoto：“改良的两层保存方法（PEC/京都溶液）在种猪入口隔离中的改进”移植论文集。

Masaaki Miyamoto et al.: "Current progress and perspectives in cell therapy for diabete mellitus"Human Cell. 14(4). 293-300 (2001)

Masaaki Miyamoto 等人：“糖尿病细胞疗法的当前进展和前景”Human Cell。

Masaaki Miyamoto, et al.: "Development of Fluoresce in Diabetate and Ethiduim Bromide Assay for islets"Cell Transplantation. 9. 681-686 (2000)

Masaaki Miyamoto 等人：“糖尿病荧光的开发和胰岛溴化乙锭测定”细胞移植。

宮本 正章: "重症難治性糖尿病性壊疸に対する自己骨髄幹細胞浸透人工真皮を用いた血管再生治療"compliation 8/1特集"糖尿病と足". (印刷中).

Masaaki Miyamoto：“使用自体骨髓干细胞浸润的人造真皮治疗严重难治性糖尿病坏疽的血管再生治疗”编译 8/1 “糖尿病与足部”专题（正在印刷中）。