Establishment of bioartificial pancreas using PTD-FNK protein

利用PTD-FNK蛋白建立生物人工胰腺

• 批准号: 15590695
• 负责人: MIYAMOTO Masaaki
• 金额: $ 2.24万
• 依托单位: Nippon Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15590695/
• 关键词: Therapeutic Angiogenesis; DDS; bioartificial pancreas; PTD-FNK; アポトーシス; b-FGF; 糖尿病; 医療用ウジ治療; 医療用ウジ治療(MDT); ハイドロゲル; 骨髄幹細胞; stem cell

We investigated efficacy and safety of autologous bone marrow-mononuclear cells plus platelets including endothelial progenitor cells (EPCs) implantation for recovering refractory chronic peripheral arterial disease (PAD) using visual and quantitative analyses by ^<99m>Tc-tetrofosmin (TF) perfusion scintigraphy, and also investigated various quantitative assessments objectively. We performed consecutive twelve cases and 19 limbs and hands with severe chronic PAD that almost Fontaine class IV(11/12 cases, about 92%) in this trial. This treatment was very effective to relieve severe pain of PAD, especially for Buerger's disease. We used visual analog scale (VAS) for measurement of pain level. The maximum pain level before implantation was 66.5±5.0(mm), and decreased to 12.1±2.2(mm) after implantation (p<0.001). Rest pain in legs and fingers were resolved eleven cases (11/12 cases, 92%). All patients could measure pain-free walking time on treadmill were improved remarkably (before implan … More tation 140±53 sec versus after implantation 451±74 sec, p=0.034). And resting ankle-brachial pressure index (ABI) in legs implanted with bone marrow-mononuclear cells was also improved (before implantation was 0.65±0.08 versus after implantation was 0.73±0.07, p=0.055).According to ^<99m>Tc-TF perfusion scintigraphy, in proximal area (region from knee to ankle) before implantation was 1.32±0.10 versus after implantation was 1.56±0.11, p=0.007). ^<99m>Tc-TF perfusion scintigraphy, in distal area (region from ankle to end of toes, or from wrist to end of fingers) before implantation was 0.79±0.06 versus after implantation was 0.83±0.06, p=0.29). Ischemic legs and hands that were injected were increased perfusion blood flow. ^<99m>Tc-TF perfusion scintigraphy was effective to estimate visual and quantitative analysis of collateral vessels in neovascularization. Thus we successed to this new treatment for most severe chronic PAD unable to cure by present any treatments, this therapeutic angiogenesis would be a new strategy for saving severe ischemic limbs and hands. Less

我们使用 ^<99m> perf的secintraphy研究了自体骨骨髓单核和血小板的自体骨骨髓单核和血小板，以及用于难治性慢性外周动脉疾病（PAD）的血小板（EPCL）抗性，并使用视觉和定量分析进行了刺激性，并还调查了客观的杂物。 。为了测量疼痛水平。 ）。植入后为0.65±0.08比较为0.73±0.07，p = 0.055）。植入之前植入之前的 ^<99m> tc-tc-tc-tc-tc-tf灌注闪烁显像（从膝盖到踝部的区域）为1.32±0.10为1.32±0.10 ） > TC-TF灌注闪烁显像是估计的视觉效果，并在新生血管形成中的附带essels定量。 。 较少的。

项目成果

医療用ウジを用いた多剤耐性緑膿菌(MDRP)合併糖尿病壊疽治療

医用蛆治疗多重耐药铜绿假单胞菌并发糖尿病坏疽

• 发表时间: 2006
• 期刊: 糖尿病 (印刷中)
• 作者: 藤本啓志;宮本正章 他
• 通讯作者: 宮本正章 他

宮本 正章: "重症難治性糖尿病性壊痕に対する自己骨髄細胞浸透人工真皮を用いた血管再生治療"Complication. 8. 31-37 (2003)

Masaaki Miyamoto：“使用自体骨髓细胞浸润的人造真皮对严重难治性糖尿病疤痕进行血管再生治疗”并发症。

今なぜ「医療用ウジ治療 (MDT) 」か?

为什么现在进行“医用蛆治疗（MDT）”？

• 发表时间: 2006
• 期刊: Pharma Medica 24
• 作者: 高木 元;宮本 正章 他;宮本 正章
• 通讯作者: 宮本 正章

自然免疫:自然免疫を応用した先端医療

先天免疫：应用自然免疫的先进医疗

• 发表时间: 2004
• 作者: Masaaki Miyamoto;et al.;宮本 正章;宮本 正章
• 通讯作者: 宮本 正章

宮本 正章: "重症難治性心血管疾患に対する自己骨髄細胞を利用した新しい血管再生治療"麻酔. 52. 567-575 (2003)

Masaaki Miyamoto：“使用自体骨髓细胞治疗严重难治性心血管疾病的新血管再生治疗”麻醉，52. 567-575 (2003)。