CELL BIOLOGICAL ANALYSIS OF ADHESIVE MECHANISM OF PERITONEUM AND PLEURA

腹膜与胸膜粘附机制的细胞生物学分析

• 批准号: 12670011
• 负责人: SASAKI Katsunori
• 金额: $ 2.18万
• 依托单位: SHINSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670011/
• 关键词: CELLULAR ADHESION MOLECULES; PERITOPNEUM; PLEURA; IMMUNO SEM; 大網; 肺胞; 接着分子; 免疫リポソーム; マウス

The failure of bio-defence of the peritoneal and pleural cavity, which is closely associated with cellular adhesion molecules, causes fatal damage to the body such as sepsis, but even if healing, severe adhesion of the serous membranes occurs inevitably and leads to intestinal obstruction or inhibition of the respiratory movement. In this project, the mechanism of the bio-defence and inhibition of adhesion in both cavities are analyzed with cell biological methods and discussed.1. Mechanism of the bio-defence of the peritoneal and pleural cavities. Interactions between ICAM-1 and LFA-1 & Mac-1 in normal, between VCAM-1 and VLA-4 in addition to ICAM-1 and LFA-1 in moderate inflammation and between fibronectin and VLA-4 in addition to VCAM-1 and VLA-4 in severe inflammation work according to each inflammatory stage. The first interaction between ICAM-1 and LFA-1 & Mac-1 promotes light adhesion, is done by microvilli of the mesothelial cells and microvilli or ruffles of leukocytes and pro … More bably used for migration. The second interaction between VCAM-1 and VLA-4 is necessary for fixation of leukocytes, which causes local secretion of cytokines. The last interaction brings about pathological adhesion through fibrous networks due to fibronectin.2. Source of leukocytes. In the peritoneal cavity, milky spots are well known as the supplying site for leukocytes. The area is always activated. Expression of ICAM-1 is four times higher than other areas. Leukocytes proliferate at that region. In the pleural cavity, the connective tissue near the costae including vascular network and adipocytes is identical to milky spots. The mesothelial cells at that region proliferate markedly, which suggest they are stem cells.3. Inhibition of migration and adhesion. In the peritoneal cavity, ICAM-1, VCAM-1, VLA-4, LFA-1,Mac-1 are blocked with antibodies, which cause drastic inhibition of leukocyte migration. From this result and previous observations, inhibition of VCAM-1 and VLA-4 may be proposed to suppress adhesion of serous membranes. Less

与细胞粘附紧密相关的腹膜和脑腔腔的生物防御失败会造成致命的损害，败血症等人体，但即使愈合，浆液膜的严重粘附也导致不可避免地会导致不可避免地会导致肠梗阻。或抑制呼吸道运动LFA-1和MAC-1在正常情况下，除了ICAM-1和LFA-1之间，在中度炎症中以及纤连蛋白ND VLA-4之间除了VCAM-1和VCAM-1和VLA-4之间，在每种炎症的严重炎症中，除了VCAM-1和VLA-4之外阶段。 cass的局部秘密是由于腹膜腔内的，因此，由腹膜造成了腹膜胸腔腔，包括脂肪细胞在内的肋骨附近的组织与乳状斑点相同。可以支撑VCAM-1和VLA-4的抑制作用以抑制浆液膜较少

项目成果

Liang Y, Sasaki K: "Expression of adhesion molecules relevant to leukocyte migration on the microvilli of liver peritoneal mesothelial cells."Anat Rec. 258. 39-46 (2000)

梁Y，佐佐木K：“与白细胞迁移相关的粘附分子在肝腹膜间皮细胞微绒毛上的表达。”Anat Rec。

Sasaki K, Johkura K, Ogiwara N, Liang Y, Cui L, Teng R, Okouchi Y, Asanuma K, Ishida O, Maruyama K: "Three-dimensional morphological analysis of antigen-antibody reaction in hepatic sinusoids preserved in hypothermic UW solution"Cryobiology. 42. 145-150 (

Sasaki K、Johkura K、Ogiwara N、Liang Y、Cui L、Teng R、Okouchi Y、Asanuma K、Ishida O、Maruyama K：“低温 UW 溶液中保存的肝窦中抗原抗体反应的三维形态学分析”

Johkura K, Liang Y, Teng R, Ogiwara N, Sasaki K.: "Nephrogenesis accopanied by vascularization in the mouse embryonic metanephros transplanted into the adult kidney for the creation of additional nephros."Nephrology. 7. 86-94 (2002)

Johkura K、Liang Y、Teng R、Ogiwara N、Sasaki K.：“移植到成人肾脏中的小鼠胚胎后肾中伴随着血管化的肾发生，用于产生额外的肾。”肾脏病学。

Liang Y, Johkura K, Ogiwara N, Sasaki K: "Expression of adhesion molecules and fibronectin of activated peritoneal surface with Lipopolysaccharide(LPS) analyzed with ImmunoSEM."Ann Anat. 183. 353-356 (2001)

Liang Y、Johkura K、Ogiwara N、Sasaki K：“用免疫扫描电镜分析脂多糖 (LPS) 激活腹膜表面粘附分子和纤连蛋白的表达。”Ann Anat。

Johkura K, Liang Y, Teng R, Ogiwara N, Sasaki K: "Nephrogenesis accopanied by vascularization in the mouse embryonic metanephros transplanted into the adult kidney for the creation of additional nephros"Nephrology. 7. 86-94 (2002)

Johkura K、Liang Y、Teng R、Ogiwara N、Sasaki K：“移植到成人肾脏中的小鼠胚胎后肾中伴随血管化的肾发生，用于产生额外的肾”肾脏病学。