STUDY AS TO INTERACTION BETWEEN IMMUNOLIPOSOME AND VASCULAR ENDOTHELIAL CELLS IN VIVO

体内免疫脂质体与血管内皮细胞相互作用的研究

基本信息

批准号：
10670002
负责人：
SASAKI Katsunori
金额：
$ 2.11万
依托单位：
SHINSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670002/
关键词：
IMMUNOLIPOSOME VASCULAR ENDOTHELIAL CELLS CELLULAR ADHESION MOLECULES BSE image ENERGY FILTER TEM 血管内皮 TEM SEM 電子分光型分析電子顕微鏡

项目摘要

To investigate interaction between immunoliposomes and vascular endothelial cells in vivo, liposomes were processed chemically into immunoliposomnes, which contained Au, Fe, or Cu particles in the interior and Polyethyleneglycol with anibody (ICAM-1orVCAM-1) outside. They were approximately 100nm in diameter and had about 30molecules on the surface, 30% of which included metals. It was confirmed in advance that prepared immunoliposomes gathered in the liver and mesenteric lympnodes with a radioisotope. They were injected into the veins of mice and rats. After 1hour they were fixed with perfusion fixation and prepared for SEM and TEM. In the liver, immunoliposomes bound to the area around the both sides of the sieve plate. Hepatic sinusoid endothelial cells ingested immunoliposomes by endocytosis, which were concentrated into the endosomes. In the lymph node, immunoliposomes adhered to high endothelial venuls (HEV), which were associated with lymphocyte homing, especially the narrow space near the junction. These results were summarized as follows:(1)Liposomes are processed easily into immunoliposomes containing metals.(2)They show some behavior of antigens on the surface in vivo.(3)They give intracellular information of ingesting antigen complex into the cytoplasm in vivo.Finely an immunoliposome injection in vivo is a useful tool to investigate cell biological dynamics of antigen express on vascular endothelial cells in vivo.

为了研究体内免疫脂质体与血管内皮细胞之间的相互作用，将脂质体化学地加工成免疫脂质体，其中包含内部和聚乙烯中的Au，Fe或Cu颗粒，以及与芳基苯乙烯（ICAM-1orvcam-1）。它们的直径约为100nm，表面约为30百万分子，其中30％包括金属。事先证实，准备了带有放射性同位素的肝脏和肠系膜淋巴结中的免疫脂质体。它们被注射到小鼠和大鼠的静脉中。 1小时后，它们用灌注固定并为SEM和TEM准备。在肝脏中，免疫脂质体与筛子两侧的区域结合。肝正正弦内皮细胞通过内吞作用摄入免疫脂质体，这些内吞作用集中在内体中。在淋巴结中，与淋巴细胞寄养有关的免疫脂质体粘附在高内皮毒液（HEV）上，尤其是在交界处附近的狭窄空间。这些结果总结如下：（1）将脂质体轻松处理成含有金属的免疫脂质体。（2）它们显示了体内表面抗原的某些行为。（3）它们提供了将抗原复合物摄入体内细胞质中的细胞内信息。在体内注射免疫脂体体是研究体内血管内皮细胞上抗原表达细胞生物动力学的有用工具。