MORPHOLOGICAL ANALYSIS OF SPECIFIC ULTRASTRUCTURES OF THE CELL MEMBRANE FORMED IN CELL-TO-CELL CONTACT ASSOCIATED WITH IMMUNOLOGICAL REACTION

与免疫反应相关的细胞间接触中形成的细胞膜的特定超微结构的形态学分析

基本信息

批准号：
04670024
负责人：
SASAKI Katsunori
金额：
$ 1.41万
依托单位：
Yamagata University (1994)Yokohama City University (1992-1993)
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1994
项目状态：
已结题

项目摘要

The project as to ultrastructural analysis of the interaction between vascular endothelial cells and leukocytes in immunological reaction, which had been carried out from 1992 to 1994, has produced the following results.In orthotopic liver transplantation and lymph node transplantation, leukocytes interacted with endothelial cells actively. It was folloewed by formation of specific ultrastructures in both cells, e.g.filamentous bridges and high density of the cell membrane. Endothelial cells, which were associated with these phenomena, are characterized by well developed Golgi apparatus, which leads to the idea that glycoproteins play an important role in this interacion.To detect glycoproteins, e.g.adhesion molecules immunohistochemically and three-dimensionally, a new method is developed here. One feature of this method was that immunoreaction for EM (electron microscopy) preparation is finished in UW solution, organ preserving solution, before fixation. After the reaction, usual treatment for EM is possible and has guaranteed simultanous comparison between transmission EM and scanning EM and good preservation of ultrastrucure as well.The method disclosed that intercellular adhesion molecule (ICAM-1) expressed exclusively in processes and folds of high endothelial venule (HEV) cells. These structures combined with ICAM-1 functioned to trap lymphocytes, because lymphocytes often dropped into the furrows between folds and adhered to them.Finally as one conclusion from this project, though cell-to-cell interaction in in vivo immunological reaction is accomplished through interaction of adhesion molecules, ultarastructural changes of the cell membrane promate this interaction. To understand dynamism of cell-to-cell interaction, it is necessary to know not only whether molecules express or not, but also where they express in one cell three-dimensionally.

1992年至1994年开展的免疫反应中血管内皮细胞与白细胞相互作用的超微结构分析项目，取得了以下成果：在原位肝移植和淋巴结移植中，白细胞与内皮细胞相互作用活跃。。随后，两种细胞中都形成了特定的超微结构，例如丝状桥和高密度的细胞膜。与这些现象相关的内皮细胞的特征是发达的高尔基体，这导致了糖蛋白在这种相互作用中发挥重要作用的想法。为了通过免疫组织化学和三维方式检测糖蛋白，例如粘附分子，一种新方法是在这里发展起来。该方法的特点之一是，在固定之前，在UW溶液（器官保存溶液）中完成用于EM（电子显微镜）制备的免疫反应。反应后可进行常规电镜处理，保证透射电镜和扫描电镜的同时比较，并保证超微结构的良好保存。该方法揭示了细胞间粘附分子（ICAM-1）仅在高内皮细胞的突起和皱襞中表达。小静脉（HEV）细胞。这些结构与 ICAM-1 结合，起到捕获淋巴细胞的作用，因为淋巴细胞经常掉入褶皱之间的沟渠中并粘附在其上。最后作为该项目的一个结论，尽管体内免疫反应中的细胞与细胞之间的相互作用是通过相互作用来完成的粘附分子的变化、细胞膜的超微结构变化促进了这种相互作用。为了了解细胞间相互作用的动态，不仅需要了解分子是否表达，还需要了解它们在一个细胞中的三维表达位置。