MORPHOLOGICAL ANALYSIS OF SPECIFIC ULTRASTRUCTURES OF THE CELL MEMBRANE FORMED IN CELL-TO-CELL CONTACT ASSOCIATED WITH IMMUNOLOGICAL REACTION

与免疫反应相关的细胞间接触中形成的细胞膜的特定超微结构的形态学分析

基本信息

批准号：
04670024
负责人：
SASAKI Katsunori
金额：
$ 1.41万
依托单位：
Yamagata University (1994)Yokohama City University (1992-1993)
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1994
项目状态：
已结题

项目摘要

The project as to ultrastructural analysis of the interaction between vascular endothelial cells and leukocytes in immunological reaction, which had been carried out from 1992 to 1994, has produced the following results.In orthotopic liver transplantation and lymph node transplantation, leukocytes interacted with endothelial cells actively. It was folloewed by formation of specific ultrastructures in both cells, e.g.filamentous bridges and high density of the cell membrane. Endothelial cells, which were associated with these phenomena, are characterized by well developed Golgi apparatus, which leads to the idea that glycoproteins play an important role in this interacion.To detect glycoproteins, e.g.adhesion molecules immunohistochemically and three-dimensionally, a new method is developed here. One feature of this method was that immunoreaction for EM (electron microscopy) preparation is finished in UW solution, organ preserving solution, before fixation. After the reaction, usual treatment for EM is possible and has guaranteed simultanous comparison between transmission EM and scanning EM and good preservation of ultrastrucure as well.The method disclosed that intercellular adhesion molecule (ICAM-1) expressed exclusively in processes and folds of high endothelial venule (HEV) cells. These structures combined with ICAM-1 functioned to trap lymphocytes, because lymphocytes often dropped into the furrows between folds and adhered to them.Finally as one conclusion from this project, though cell-to-cell interaction in in vivo immunological reaction is accomplished through interaction of adhesion molecules, ultarastructural changes of the cell membrane promate this interaction. To understand dynamism of cell-to-cell interaction, it is necessary to know not only whether molecules express or not, but also where they express in one cell three-dimensionally.

从1992年至1994年进行的免疫反应中血管内皮细胞与白细胞之间相互作用的超微结构分析的项目已产生以下结果。原始肝移植和淋巴结淋巴结和淋巴结移植，白细胞与内皮细胞相互作用。它是通过在两个细胞中的特定超微结构（例如丝状桥和细胞膜高密度的）形成而形成的。与这些现象相关的内皮细胞的特征是发达的高尔基体，这导致了这样一种观念，即糖蛋白在该间隔中起着重要作用。检测糖蛋白，例如粘附分子，即免疫组织化学和三维方法，在这里产生了一种新方法。该方法的一个特征是，在固定前，在UW溶液（器官保存溶液）中完成了EM（电子显微镜）制剂的免疫反应。反应后，对EM的常规处理是可能的，并且可以保证EM和扫描EM之间的同时比较以及对超疗法的良好保存。该方法披露了细胞间粘附分子（ICAM-1）在高端细胞（HEV）细胞的过程和折叠中独家表达。这些结构与ICAM-1结合在诱捕淋巴细胞中的作用，因为淋巴细胞通常掉入褶皱之间的犁沟中并粘附在它们之间。从该项目中的一个结论中，尽管细胞与细胞之间的相互作用在体内免疫反应是通过粘附分子的相互作用，而是在体内免疫反应，最终的分裂效果使得这种细胞相互作用。要了解细胞对细胞相互作用的动力学，不仅有必要知道分子是否表达，而且还必须知道它们在一个三维中表达的位置。