Functional roles of macrophage populations appearing in the renal fibrosis

巨噬细胞群在肾纤维化中的功能作用

基本信息

批准号：
12660287
负责人：
YAMATE Jyoji
金额：
$ 2.56万
依托单位：
OSAKA PREFECTURE UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

项目摘要

Macrophagss show heterogeneous functions, consisting of three major types such as exudate macrophages, resident macrophages (histiocytes), and antigen-presenting dendritic cells. This study investigated the properties and participation of macrophage populations in the renal fibrosis.1. Properties of macrophages appearing in chronic renal fibrosis: In the chronic renal fibrosis, that had been induced by repeated injections of cisplatin with nephrotoxicity, exudate and resident macrophages increased at the early stages, and then decreased at the late stages. On the other hand, dendritic cells appeared in greater number at both early and late stages. Because lymphocytes were often present in the late stagss, MHC class II-expressing dendritic cells may be related to induction of lymphocytes.2. Effects of taurine on rat renal fibrosis model: In cisplatin-induced rat renal fibrosis, that had been treated with taurine with antioxidant properties, macrophage numbers were decreased, resulting in reduced fibrosis.3. Characteristics of macrophage populations in thioacetoamide (TAA)-induced rat hepatic fibrosis: In order to compare with macrophagss in renal fibrosis, we investigated lesions of TAA- induced renal fibrosis, with special reference to macrophage populations. In hepatic fibrosis, exudate and resident macrophages, and dendritic cells were present, but there were differences in time points of appearance and distribution between these macrophage populations. In contrast to renal fibrosis, Kupffer cells seemed to play an important role in hepatic fibrosis, and lymphocytes were smaller in number, even though MHC class II-expressing dendritic cells were increased.4. Establishment of in vitro cell model for renal fibrosis: We attempted to establish a cell line (KB-D8) from rat thymus-derived dendritic cell sarcoma. KB-D8 might be useful for studies on dendritic cell nature relating to renal fibrosis.

巨噬菌显示出异质的功能，由三种主要类型组成，例如渗出巨噬细胞，驻留巨噬细胞（组织形成细胞）和抗原呈递抗原的树突状细胞。这项研究研究了巨噬细胞种群在肾纤维化中的特性和参与。1。慢性肾纤维化中出现的巨噬细胞的特性：在慢性肾纤维化中，这是由于反复注射顺铂具有肾毒性，渗出液和常驻巨噬细胞在早期阶段增加的，然后在晚期降低。另一方面，树突状细胞在早期和晚期均出现更多。由于淋巴细胞经常存在于晚期雄鹿中，因此表达MHC II类的树突状细胞可能与淋巴细胞的诱导有关2。牛磺酸对大鼠肾纤维化模型的影响：在顺铂诱导的大鼠肾纤维化中，已用牛磺酸治疗具有抗氧化特性的牛磺酸，巨噬细胞数量减少，导致纤维化降低。3。巨噬细胞种群（TAA）诱导的大鼠肝纤维化的巨噬细胞特征：为了与肾纤维化中的巨噬细胞进行比较，我们研究了TAA诱导的肾纤维化病变，并特别提及巨噬细胞。在肝纤维化中，存在渗出液和驻留巨噬细胞以及树突状细胞，但是这些巨噬细胞种群之间的外观和分布时间存在差异。与肾纤维化相反，库普弗细胞似乎在肝纤维化中起重要作用，即使淋巴细胞的数量较小，即使MHC II类表达的树突状细胞增加了。4。建立用于肾纤维化的体外细胞模型：我们试图从大鼠胸腺衍生的树突状细胞肉瘤中建立细胞系（KB-D8）。 KB-D8对于与肾纤维化有关的树突状细胞性质的研究可能很有用。