Functional roles of macrophage populations appearing in the renal fibrosis

巨噬细胞群在肾纤维化中的功能作用

基本信息

批准号：
12660287
负责人：
YAMATE Jyoji
金额：
$ 2.56万
依托单位：
OSAKA PREFECTURE UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

项目摘要

Macrophagss show heterogeneous functions, consisting of three major types such as exudate macrophages, resident macrophages (histiocytes), and antigen-presenting dendritic cells. This study investigated the properties and participation of macrophage populations in the renal fibrosis.1. Properties of macrophages appearing in chronic renal fibrosis: In the chronic renal fibrosis, that had been induced by repeated injections of cisplatin with nephrotoxicity, exudate and resident macrophages increased at the early stages, and then decreased at the late stages. On the other hand, dendritic cells appeared in greater number at both early and late stages. Because lymphocytes were often present in the late stagss, MHC class II-expressing dendritic cells may be related to induction of lymphocytes.2. Effects of taurine on rat renal fibrosis model: In cisplatin-induced rat renal fibrosis, that had been treated with taurine with antioxidant properties, macrophage numbers were decreased, resulting in reduced fibrosis.3. Characteristics of macrophage populations in thioacetoamide (TAA)-induced rat hepatic fibrosis: In order to compare with macrophagss in renal fibrosis, we investigated lesions of TAA- induced renal fibrosis, with special reference to macrophage populations. In hepatic fibrosis, exudate and resident macrophages, and dendritic cells were present, but there were differences in time points of appearance and distribution between these macrophage populations. In contrast to renal fibrosis, Kupffer cells seemed to play an important role in hepatic fibrosis, and lymphocytes were smaller in number, even though MHC class II-expressing dendritic cells were increased.4. Establishment of in vitro cell model for renal fibrosis: We attempted to establish a cell line (KB-D8) from rat thymus-derived dendritic cell sarcoma. KB-D8 might be useful for studies on dendritic cell nature relating to renal fibrosis.

巨噬细胞表现出异质性功能，由三种主要类型组成，例如渗出型巨噬细胞、常驻型巨噬细胞（组织细胞）和抗原呈递树突状细胞。本研究探讨了巨噬细胞群的特性及其在肾纤维化中的参与情况。 1．慢性肾纤维化出现的巨噬细胞特性：反复注射有肾毒性的顺铂引起的慢性肾纤维化，渗出物和常驻巨噬细胞早期增多，后期减少。另一方面，树突状细胞在早期和晚期都出现较多数量。由于淋巴细胞常出现在晚期，表达MHC II类的树突状细胞可能与淋巴细胞的诱导有关。 2．牛磺酸对大鼠肾纤维化模型的影响：在顺铂诱导的大鼠肾纤维化模型中，用具有抗氧化作用的牛磺酸处理后，巨噬细胞数量减少，纤维化程度减轻。 3．硫代乙酰胺（TAA）诱导的大鼠肝纤维化中巨噬细胞群的特征：为了与肾纤维化中的巨噬细胞进行比较，我们研究了TAA诱导的肾纤维化的病变，特别是巨噬细胞群。在肝纤维化中，存在渗出物和常驻巨噬细胞以及树突状细胞，但这些巨噬细胞群之间的出现和分布时间点存在差异。与肾纤维化相反，库普弗细胞似乎在肝纤维化中发挥着重要作用，尽管表达MHC II类的树突状细胞增多，但淋巴细胞数量较少。4．肾纤维化体外细胞模型的建立：我们尝试从大鼠胸腺来源的树突状细胞肉瘤中建立细胞系（KB-D8）。 KB-D8 可能有助于研究与肾纤维化相关的树突状细胞性质。