THE HISTOGENESIS OF MALIGNANT FIBROUS HISTIOCYTOMA (MFH), WITH THE SPECIAL REFERENCE TO MULTIPOTENTIAL DIFFERENTIATION OF MFH CELLS

恶性纤维组织细胞瘤（MFH）的组织发生，特别是 MFH 细胞的多向分化

• 批准号: 07660424
• 负责人: YAMATE Jyoji
• 金额: $ 1.28万
• 依托单位: OSAKA PREFECTURE UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07660424/
• 关键词: malignant fibrous histiocytoma; mesenchymal progenitor; mesenchymal tumor; myofibroblastic cell; histiocyte; histogenesis; multipotential differentiation; rat experimental model; 悪意線維性組織球腫

The histogenesis of malignant fibrous histiocytoma (MFH) is still undetermined. We have investigated the histogenesis using cloned cell lines (MT-8, undifferentiated mesenchymal cells ; MT-9, mesenchymal cells with both natures of histiocytes and fibroblasts) derived from transplantable rat MFH.(1) By adding hyperlipemic serum or lipopolysaccharide, both which are a potential stimulus to macrophage functions, to the medium, MT-8 and MT-9 cells enhanced their histiocytic natures.(2) Cisplatin (anticancer drug)-resistant cell lines (MT-R8 and MT-R9) were induced from MT-8 and MT-9 cells, respectively. MT-R8 and MT-R9 cells enhanced both histiocytic and myofibroblastic natures, and further the tumors induced in syngeneic rats by MT-R9 cells showed a variety of histology : neoplastic proliferations of myofibroblasts and granular cells, osteosarcoma-like areas and areas involving many lipoblasts.(3) We investigated the origin of "histiocytic cells" in MFH and the immunophenotypes against ra … More t histiocyte-specific antibodies (ED1 and ED2). As a result, it was demonstrated that the presence of heterogeneities in the origin and immunophenotypes of the histiocytic cells.(4) Monoclonal antibodies against MT-8 cells were produced, and the staining patterns were immunohistochemically investigated. It was clarified that there are common antigens between MFH cells and undifferentiated mesenchymal cells or macrophage liniage cells, indicating the heterogeneity in cell natures of MFH-constituting cells.(5) We established transplantable tumors and cell lines from histiocytic sarcoma, fibrosarcoma and malignant meningioma in rats, and made comparisons of cell natures between MFH cells and these mesenchymal cell lines. The results indicated marked differences in biological natures between MFH and other mesenchymal tumor cells.(6) On the basis of these results, it was concluded that MFH consists of mesenchymal cells at various differentiation stages shifting from undifferentiated cells to cells with both histiocytic and fibroblastic natures ; the cellular natures could be easily changed, presumably depending on microenvironmental/genetic factors. MFH cells may be a mesenchymal progenitor with multipotential differentiation. The cell natures appear to contribute to endless, unstable histology of MFH.(7) Rat cell lines established in this study may provide useful experimental systems for studies on the histogenesis of MFH as well as mesenchymal differentiation yet undetermined. Less

恶性纤维组织组织（MFH）的组织发生仍未确定。我们已经使用克隆细胞系（MT-8，未分化的间充质细胞； MT-9，均具有组织细胞和成纤维细胞的生成型）来研究了组织发生。 MT-8和MT-9细胞增强了其组织细胞性质。（2）顺铂（抗癌药）抗药性细胞系（MT-R8和MT-R9）分别从MT-8和MT-9细胞诱导。 MT-R8 and MT-R9 cells enhanced both histiocytic and myofibroblastic natures, and further the tumors induced in syngeneic rats by MT-R9 cells shown a variety of histology: neoplastic proliferations of myofibroblasts and granular cells, osteosarcoma-like Areas and areas involved many lipoblasts.(3) We investigated the origin of "histiocytic cells" in MFH和针对RA的免疫表型……更多的组织细胞特异性抗体（ED1和ED2）。结果，证明了组织细胞细胞的起源和免疫表型中的异质性。（4）对MT-8细胞的单克隆抗体产生了对MT-8细胞的作用，并对染色模式进行了免疫组织化学研究。 was clarified that there are common antigens between MFH cells and undifferentiated mesenchymal cells or macrophage liniage cells, indicating the heterogeneity in cell natures of MFH-constituting cells.(5) We established transplantable tumors and cell lines from histiotic sarcoma, fibrosarcoma and malignant meningioma in rats, and made comparisons of cell natures between MFH cells这些间充质细胞系。结果表明，MFH和其他间充质肿瘤细胞之间的生物学性质显着差异。（6）在这些结果的基础上，得出的结论是，MFH由各种分化阶段组成的各种分化阶段，从未分化的细胞转移到细胞的细胞中，具有荷兰和成纤维细胞分布细胞；细胞性质可以很容易地改变，大概取决于微环境/遗传因素。 MFH细胞可能是具有多能分化的间充质祖细胞。 （7）本研究中建立的大鼠细胞系似乎有助于无尽的，不稳定的组织学，可能为MFH的组织发生以及间充质分化尚未确定尚未确定的研究提供了有用的实验系统。较少的

项目成果

yamato J: "Phenotypic modulation in cisplatin-resistant cloned cells derived from transplantable rat malignant fibrous histiocytoma" Pathology International. 46. 557-567 (1996)

yamato J：“源自可移植大鼠恶性纤维组织细胞瘤的顺铂耐药克隆细胞的表型调节”国际病理学。

Yamate J et al: "Phenotypic changes in lipopolysaccharide-treated cloned cells derived from transplantable rat malignant fibrous histiocytoma" The Journal of Veterinary Medical Science. 58. 1017-1020 (1996)

Yamate J 等人：“源自可移植大鼠恶性纤维组织细胞瘤的经脂多糖处理的克隆细胞的表型变化”《兽医医学科学杂志》。

Yamate J et al: "Phenotypic modulation in cisplatin-resistant cloned cells derived from transplantable rat malignant fibrous histiocytoma" Pathology International. 46. 557-567 (1996)

Yamate J 等人：“源自可移植大鼠恶性纤维组织细胞瘤的顺铂耐药克隆细胞的表型调节”国际病理学。

Yamate J: "Heterogeneity in the origin and immunophenotypes of "histiocytic" cells in transplantable rat malignant fibrous histiocytoma" The Journal of Veterinary Medical Science. 58. 603-609 (1996)

Yamate J：“可移植大鼠恶性纤维组织细胞瘤中“组织细胞”细胞的起源和免疫表型的异质性”《兽医医学科学杂志》。

Tsujino K: "Establishment and characterization of cell lines derived from a transplantable rat malignant meningioma: morphological heterogeneity and production of nerve growth factor (NGF)" Acta Neuropathologica. (in press). (1997)

Tsujino K：“源自可移植大鼠恶性脑膜瘤的细胞系的建立和表征：形态异质性和神经生长因子（NGF）的产生”《神经病理学报》。