Immune response at the fetal-maternal interface during normal gestation

正常妊娠期间胎儿-母体界面的免疫反应

基本信息

批准号：
08045058
负责人：
YAMATE Jyoji
金额：
$ 4.42万
依托单位：
Osaka Prefecture University
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1998
项目状态：
已结题

项目摘要

1. The kinetics of uterine NK cells under chemokine-included extracellular matrix and apoptosis-induced factors was clarified by isolating the cells using magnatic cell isolation methods.2.The differentiation of progenitor cells of uterine NK cells was investigated in vitro by addition of a various kinds of cytokines/chemokines, with special references to cytolysis, and morphology and nature of cytoplamic granules of NK cells.3.CD3e transgenic mice would be useful for investigation of NK cell function. Using this animal model, interactions of VCAM and ICAM on vascular endothelia to uterine NK cells was examined. Furthermore, the relationship between uterine NK cell differentiation and abortion due to uncomparable MHC class II or cytokine injection was investigated ; this clarified close relationship of habitual abortion to NK cells.4.Immune response at the fetal-maternal interface is a definite factor for establishment of gestation through placenta formation from fertilized ovary implantation. These studies provided the in vivo data of possible regulation of implantation ; these data lead not only to clarification of pathogenesis of habitual abortion but also to production of animal models of the habitual abortion.5.In order to continue and promote this project, one professor of Osaka prefecture University went to Argentina (Univ. BuenosAires), U.S.A.(Univ. Harvard, and Univ. California), and Canada (Univ. Guelph). Some professors, Univ. Guelph, were invited to Osaka Prefecture University.

1. 通过磁力细胞分离方法分离细胞，阐明了子宫 NK 细胞在包含趋化因子的细胞外基质和凋亡诱导因子作用下的动力学。 2. 通过添加各种细胞因子/趋化因子，特别是NK细胞的细胞溶解、细胞质颗粒的形态和性质。 3.CD3e转基因小鼠可用于研究 NK 细胞功能。使用该动物模型，检查了血管内皮细胞上的 VCAM 和 ICAM 与子宫 NK 细胞的相互作用。此外，还研究了子宫NK细胞分化与由于无与伦比的MHC II类或细胞因子注射引起的流产之间的关系；这阐明了习惯性流产与NK细胞的密切关系。4.母胎界面的免疫反应是受精卵巢着床后胎盘形成妊娠建立的明确因素。这些研究提供了可能的植入调节的体内数据；这些数据不仅有助于阐明习惯性流产的发病机制，而且有助于制作习惯性流产的动物模型。 5.为了继续和推进该项目，大阪府立大学的一位教授前往阿根廷（Univ. BuenosAires），美国（哈佛大学、加州大学）和加拿大（圭尔夫大学）。一些教授，大学。圭尔夫博士受邀前往大阪府立大学。