Discovery of a pigment produced by Streptococcus pyogenes

发现化脓性链球菌产生的色素

基本信息

批准号：
10680293
负责人：
MICHAEL J FEDERLE
金额：
$ 19.35万
依托单位：
UNIVERSITY OF ILLINOIS AT CHICAGO
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-03-01 至 2025-02-28
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10680293
关键词：
Acetyltransferase Acute Air Anabolism Antibiotic Therapy Bacteria Carotenoids Cell Density Cells Chemicals Chromatography Circulation Clinical Color Enzymes Event Exposure to Exudate Gene Family Genes Genetic Screening Growth Hand Human Hyperpigmentation Immune Infection Inflammatory Laboratories Ligase Lipids Literature Macrophage Methods Mutagenesis Nasopharynx Neutrophil Infiltration Nuclear Magnetic Resonance Operon Oranges Organism Oxidative Stress Oxygen Pathogenesis Pathway interactions Pharyngeal structure Phenotype Pigments Polyenes Production Property Pseudomonas aeruginosa Reactive Oxygen Species Recurrence Regulation Respiratory Burst Serotyping Skin Skin colonization Staphylococcus aureus Streptococcus Group B Streptococcus pyogenes Stress Structure Testing Tonsil Vaccines Virulence Virulence Factors Work antimicrobial bacterial fitness cell killing cytotoxic dehydrogenation environmental stressor fitness glycosylation glycosyltransferase human pathogen microorganism mutant new therapeutic target pathogen pathogenic bacteria stressor

Acetyltransferase Acute Air Anabolism Antibiotic Therapy Bacteria Carotenoids Cell Density Cells Chemicals Chromatography Circulation Clinical Color Enzymes Event Exposure to Exudate Gene Family Genes Genetic Screening Growth Hand Human Hyperpigmentation Immune Infection Inflammatory Laboratories Ligase Lipids Literature Macrophage Methods Mutagenesis Nasopharynx Neutrophil Infiltration Nuclear Magnetic Resonance Operon Oranges Organism Oxidative Stress Oxygen Pathogenesis Pathway interactions Pharyngeal structure Phenotype Pigments Polyenes Production Property Pseudomonas aeruginosa Reactive Oxygen Species Recurrence Regulation Respiratory Burst Serotyping Skin Skin colonization Staphylococcus aureus Streptococcus Group B Streptococcus pyogenes Stress Structure Testing Tonsil Vaccines Virulence Virulence Factors Work antimicrobial bacterial fitness cell killing cytotoxic dehydrogenation environmental stressor fitness glycosylation glycosyltransferase human pathogen microorganism mutant new therapeutic target pathogen pathogenic bacteria stressor

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项目摘要

Discovery of a pigment produced by Streptococcus pyogenes Abstract This proposal seeks to advance a new observation that the human pathogen Streptococcus pyogenes (Group A Streptococcus, GAS) produces a pigment that has gone unreported. We hypothesize the pigment protects GAS from host-derived and/or environmental stressors (such as reactive oxygen species) and likely enhances its fitness and potentially augments its virulence. For 80 years, no reference in the literature exists of a pigment being produced by this important organism, and prior to that, only two citations allude to such a factor. We suspect this property has been overlooked because its presence only becomes apparent in the laboratory after culturing in a chemically defined medium, grown to high cell densities, and after exposure to oxygen. Understanding if and how the production of a pigment could boost the fitness of GAS could provide a new target that therapeutics or vaccines could take aim upon. The objectives of this proposal are to elucidate the composition and structure of the GAS pigment, to identify genes associated with its production and regulation, and to characterize its ability to protect GAS from stressors and immune cell killing while exploring any cytotoxic properties.

发现链球菌生产的色素抽象的该提案旨在推进人类病原体链球菌为化脓性的新观察（组）气体链球菌会产生一种未报告的色素。我们假设颜料保护来自宿主衍生和/或环境压力源（例如活性氧）的气体，可能会增强它的健身并有可能增强其毒力。 80年来，文献中不存在颜料的参考由这种重要生物产生，在此之前，只有两个引用暗示了这一因素。我们怀疑该物业被忽略了，因为它的存在只有在实验室才明显在化学定义的培养基中培养，生长至高细胞密度，并在暴露于氧气后。了解颜料的生产是否以及如何增强气体的适应性可以提供新的针对治疗剂或疫苗可以针对的。该提案的目标是阐明气体色素的组成和结构，以识别与其生产和调节相关的基因并表征其保护气体免受压力源和免疫细胞杀死的能力，同时探索任何细胞毒性特性。