Basic study for modulation of macrophage function by ELF electric field.

ELF电场调节巨噬细胞功能的基础研究。

• 批准号: 11834001
• 负责人: SHIMIZU Koichi
• 金额: $ 2.24万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11834001/
• 关键词: ELF electric field; immune response; immunomodulation; macrophage; phagocytosis; displacement current; flow cytometry; 貧食能; 電気刺激_

To pursue the possibility of immunomodulation by ELF electric field exposure, the effects of the electro-stimulation on phagocytosis of macrophages were investigated in in vitro experiments. The magnitude of the electric field was in the same level as the commercial apparatus for the electric field therapy. Through this study, the following results were obtained.1. Direct stimulation with electric currentThe phagocytosis of peritoneal exudate macrophages from a mouse was studied. As phagocytized particles, fluorescent latex beads were used. The current density and the duration of the stimulation were 0.1 or 1A/m^2, and from 10 sec to 1 hour, respectively. In microscope observation, apparent reduction of phagocytosis was observed in stimulated macrophages. There was not significant difference among the results in different exposure conditions examined.2. Development of exposure system for indirect stimulationIn the commercial apparatus for the electric field therapy, the displacement cu … More rrent is generated in the human body. To simulate this indirect stimulation, the experimental container was designed to stimulate macrophages with displacement current. To supply the voltage to the container, the power source of the commercial apparatus for the electric field therapy was used. With this source, the current density of 0.35 A/m^2 was applied to the container.3. Stimulation with the displacement currentThe effect of displacement current on the phagocytosis of the macrophages was studied. The current density and the duration of stimulation were 0.35 A/m^2 (50Hz) and 30min or 1 h, respectively. An assay was made on the activity of phagocytosis in the flow cytometry. The suppression of phagocytosis was observed in stimulated macrophages. The suppression was apparent particularly in the macrophages with active phagocytosis.The results of this study suggested the possibility to suppress the phagocytosis of macrophages. This suppression can be made with the commercial apparatus for the electric field therapy. The suppression of macrophage activity will result in the modification of the total immunological response through the process of antigen presentation. Therefore, the feasibility of immunomodulation by the ELF electro-stimulation has been verified through this study. Less

为了追求通过ELF电场暴露进行免疫调节的可能性，在体外实验中研究了电刺激对巨噬细胞吞噬作用的影响。电场的大小与用于电场疗法的商业设备相同。通过这项研究，获得以下结果1。用电流直接刺激腹膜渗出症的吞噬作用是从小鼠那里进行的。作为吞噬细胞的颗粒，使用了荧光乳胶珠。当前密度和模拟持续时间分别为0.1或1a/m^2，分别为10秒至1小时。在显微镜观察中，在刺激的巨噬细胞中观察到吞噬作用明显减少。在所检查的不同暴露条件下的结果之间没有显着差异。2。开发用于间接模拟的曝光系统在电场疗法的商业设备中，流离失所Cu…在人体中产生更多的rrent。为了模拟这种间接刺激，设计实验容器以刺激巨噬细胞的位移电流。为了向容器提供电压，使用了电场疗法的商用设备的电源。使用此源，将0.35 A/m^2的电流密度应用于容器3。刺激随着电流的刺激研究了位移电流对巨噬细胞吞噬作用的影响。电流密度和模拟持续时间分别为0.35 A/m^2（50Hz）和30分钟或1小时。对流式细胞仪中吞噬作用的活性进行了测定。在刺激的巨噬细胞中观察到吞噬作用的抑制。这项研究的结果表明有可能抑制巨噬细胞的吞噬作用。可以使用用于电场疗法的商业设备进行这种抑制。巨噬细胞活性的抑制作用将通过抗原表现过程来改变总免疫反应。因此，通过这项研究验证了通过ELF电刺激免疫调节的可行性。较少的

项目成果

清水久恵 他: "ELF電界曝露に伴う生理的変化に関する基礎的検討-ヒト体表における血流変化の可能性-"電子情報通信学会技術研究報告. EMCJ99-97. 49-53 (1999)

Hisae Shimizu 等人：“与 ELF 电场暴露相关的生理变化的基础研究 - 人体表面血流变化的可能性 -”IEICE 技术研究报告 49-53（1999）。

H.Shimizu, et al.: "Effects of ELF electric field on physiological function"Proceedings of ISAP 2000. in press. (2000)

H.Shimizu 等人：“ELF 电场对生理功能的影响”，ISAP 2000 年报，出版中。

山下政司 他: "ELF強電界中における生体信号の計測および解析"電気情報通信学会技術研究報告. MBE2000-66. 95-100 (2000)

Masashi Yamashita等人：“ELF强电场中生物信号的测量和分析”日本电气工程师学会MBE2000-66（2000）的技术报告。

T.Shimooka, et al.: "ELF electrostimulation on macrophages : possibilty of immunomodulation by electric field"Proceedings of the 22nd Annual International Conference of the IEEE Engineering in Medicine and Biology Society. (CR-ROM). (2000)

T.Shimooka 等人：“巨噬细胞上的 ELF 电刺激：通过电场进行免疫调节的可能性”IEEE 医学和生物学工程学会第 22 届国际年会论文集。

H.Shimizu, J.Arisawa and K.Shimizu: "Fundamental study on physiological change associated with ELF electric field exposure=possibility of blood flow change in human body="Technical Report of IEICE. EMCJ99-97. 49-53 (1999)

H.Shimizu、J.Arisawa 和 K.Shimizu：“与 ELF 电场暴露相关的生理变化的基础研究=人体血流变化的可能性=”IEICE 技术报告。