Ultra High-Performance Liquid Chromatography High-Resolution Mass Spectrometer (UHPLC-HRMS)

超高性能液相色谱高分辨率质谱仪 (UHPLC-HRMS)

• 批准号: 529714284
• 金额: --
• 依托单位: Friedrich-Schiller-Universität Jena
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2023
• 资助国家: 德国
• 起止时间: 2022-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/529714284?language=en
• 关键词: Ultra; Performance; Liquid; Chromatography; Resolution

We apply for an Ultra High-Performance Liquid Chromatography High-Resolution Mass Spectrometer for the metabolomic investigation of microorganisms and microbial communities. The instrument will mainly serve to monitor the metabolic responses of microorganisms to different interaction partners within communities and to identify signaling metabolites that regulate the community structure. Model communities from laboratory experiments as well as samples from mesocosm and fieldwork will be investigated by exploiting the power of modern mass spectrometry. The instrument will contribute to a platform for several groups organized within the CRCs ChemBioSys and AquaDiva and it will be part of the instrument park of the EXC Balance of the Microverse. Metabolomics is a mature technique that allows the monitoring of metabolic changes within given biological systems. It serves as a method for hypothesis generation by identification of metabolic pathways that are regulated in response to external factors, such as stress or the presence of interaction partners. With powerful chemoinformatic support, large datasets can be mined for metabolites that are candidate chemical mediators. Especially the high resolution of modern mass spectrometers and the fast scan rate allows for the comprehensive characterization of metabolites. The sensitivity of the newest instrument generation will allow, on the one hand, to address even trace compounds within complex natural samples but also enable miniaturization and monitoring of samples generated, e.g., from microfluidics experiments. The resulting mass spectrometric data will also form the basis for the development of new chemoinformatics approaches for computer-aided structure elucidation. On the one hand, this instrument will thus advance research in method-developing analytical chemistry and support activities in the department of bioorganic analytics. On the other hand, it will also enable collaborative projects within larger consortia within the faculties of biosciences, faculty of chemistry and earth sciences and the faculty for mathematics and informatics. The device will thus have an important function at the Jena site as a platform technology for numerous research activities.

我们申请超高性液相色谱高分辨率质谱仪，以对微生物和微生物群落的代谢组学研究。该仪器将主要用于监测微生物对社区内不同互动伙伴的代谢反应，并确定调节社区结构的信号代谢物。通过利用现代质谱法的力量来研究实验室实验的模型社区以及中验和现场工作的样本。该仪器将为在CRCS Chembiosys和Aquadiva中组织的几个组的平台做出贡献，它将成为微versecter exc平衡的仪器公园的一部分。代谢组学是一种成熟的技术，可以监测给定生物系统内的代谢变化。它通过鉴定对外部因素（例如压力或相互作用伙伴的存在）来调节的代谢途径来作为假设产生的方法。有了强大的化学信息支持，可以为候选化学介质的代谢产物开采大型数据集。尤其是现代质谱仪的高分辨率和快速扫描速率允许代谢物的全面表征。一方面，最新仪器生成的敏感性将允许在复杂的天然样品中解决甚至痕量的化合物，但还可以从微流体实验中对产生的样品进行微型化和监测。所得的质谱数据还将为开发用于计算机辅助结构的新化学信息学方法的基础。一方面，该工具将在生物有机分析部的方法开发分析化学和支持活动方面进行研究。另一方面，它还将在生物科学学院，化学和地球科学学院以及数学和信息学教师的范围内实现较大财团内的合作项目。因此，该设备将在Jena网站上具有重要功能，作为众多研究活动的平台技术。