Tandem mass spectrometer coupled to an ultra-high performance liquid chromatograph: UHPLC-HRMS/MS

串联质谱仪与超高性能液相色谱仪联用：UHPLC-HRMS/MS

• 批准号: 518143553
• 金额: --
• 依托单位: Friedrich-Alexander-Universität Erlangen-Nürnberg
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2023
• 资助国家: 德国
• 起止时间: 2022-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/518143553?language=en
• 关键词: Tandem; mass; spectrometer; coupled; ultra

With the present application for large-scale equipment funding for a UHPLC-HRMS/MS system, the Institute of Bioprocess Engineering (BVT) is pursuing essential goals for further development: (1) The consolidation of successful projects in the field of enzymatic catalysis and (2) the use of natural substances with biological activity, as well as (3) the development of systems biology projects in the field of microbial biotechnology and animal cell culture. The device described here is essential for the planned work of the two newly appointed professors Kathrin Castiglione (Bioprocess Engineering) and Sebastian Aljoscha Wahl (Systems Biotechnology) in 2018 and 2022. The device will enable precise analyses in the field of small molecules for the identification and quantification of substances in reaction mixtures, natural substances, media as well as in cells (metabolomics). Furthermore, the device will enable the measurement of protein composition. Measurements of such complex molecules in complex samples can only be achieved with a high-resolution, sensitive system. The procurement is financially supported by the university in the form of appointment commitments. The aim of planned research is to develop and apply tools for: (1) the investigation and optimisation of complex biocatalytic processes, (2) the identification of bioactive natural substances for the development of natural defense strategies against viruses and bacteria, and (3) the systems biology analysis of (animal) cells. The knowledge of complex catalyses, bioprocesses, cultivation, metabolism and modelling available at the institute offers a very good starting point for addressing new scientific questions with the help of HR-MS/MS.

With the present application for large-scale equipment funding for a UHPLC-HRMS/MS system, the Institute of Bioprocess Engineering (BVT) is pursuing essential goals for further development: (1) The consolidation of successful projects in the field of enzymatic catalysis and (2) the use of natural substances with biological activity, as well as (3) the development of systems biology projects in the field of microbial biotechnology and animal cell culture.此处描述的设备对于2018年和2022年的两位新任命的教授Kathrin Castiglione（Bioprocess Engineering）和Sebastian Aljoscha Wahl（Systems Biotechnology）的计划工作至关重要。该设备将在小分子的识别和量化中量身定量的量化，以供应，并在自然中进行量化，并启用精确的精确分析。 （代谢组学）。此外，该设备将能够测量蛋白质成分。复杂样品中这种复杂分子的测量只能通过高分辨率，敏感的系统来实现。该采购得到了大学以任命承诺的形式进行财务支持。计划研究的目的是开发和应用工具：（1）复杂生物催化过程的研究和优化，（2）生物活性天然物质的鉴定，以开发针对病毒和细菌的自然防御策略，以及（3）动物细胞的系统生物学分析。研究所提供的复杂催化剂，生物处理，培养，新陈代谢和建模的知识为解决新科学问题提供了一个很好的起点。