ROLES OF FIBROBLAST GROWTH FACTOR (B-FGF) AND GAP JUNCTION ON NEOCORTICAL HISTOGENESIS

成纤维细胞生长因子 (B-FGF) 和间隙连接对新皮质组织发生的作用

基本信息

批准号：
11670784
负责人：
TAKAHASHI Takao
金额：
$ 2.5万
依托单位：
KEIO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670784/
关键词：
Cerebral Histogenesis Mouse Cell Cycle Tissue Culture Gap Junction Basic Fibroblast Growth Factor Octanol Mitogen Antimitogen GAP Junction FGF2 細胞分裂 Q Fraction 組織培養

项目摘要

We have examined the cell cycle progression in explants cultured with FGF-2 or 1-octanol. FGF-2 is representative of physiologic mitogens respecting the neocortical PVE.1-octanol, on the other hand, has been demonstrated to mediate a countering antimitogenic effect through gap junction blockade.In vivo, there are two options for postmitotic cells, either to exit the cycle and migrate out of the VZ (fate to Q fraction) or to re-enter S phase and continue to cycle (fate to P fraction). In the explants, by contrast, a substantial proportion makes neither of these choices but instead persists in the VZ in an indeterminate state (I fraction). The phenomenon is modulated, in opposing directions and to different degree, by FGF-2 and 1-octanol. Modulation by these mitogenic and antimitogenic agents is observed only in the more advanced region of the neurogenetic gradient. The phenomenon is a relatively selective one in that the majority of other vital properties of the epithelium are little altered : the epithelium maintains its architectonic integrity and there is no interruption of interkinetic nuclear migration, respecting cell cycle phases. Moreover, there is no augmentation in the generally low occurrence rate of pyknosis. That is, these conditions of culture appear to have unmasked relatively selectively mechanisms regulatory to the proliferative fates of cells of the PVE.Importantly, mechanisms by which this effect is modulated by FGF-2 or 1-octanol are evidently developmentally regulated, expressed only in the relatively advanced region of the neurogenetic gradient.

我们已经检查了用FGF-2或1-二二醇培养的外植体中的细胞周期进程。 FGF-2是尊重新皮质PVE的生理有丝分裂剂的代表。另一方面，已经证明1-辛醇可以通过间隙连接封锁介导相反的抗极力作用。周期和从VZ迁移（命运到Q分数）或重新进入阶段并继续循环（命运到P分数）。相比之下，在外植体中，很大一部分都没有做出这两种选择，而是在不确定状态的VZ中持续存在（i分数）。 FGF-2和1-二二酚在相反的方向和不同程度上调节该现象。仅在神经遗传学梯度的更先进的区域中观察到这些有丝分裂和抗源性剂的调节。该现象是一种相对选择性的现象，因为上皮的大多数其他重要特性几乎没有改变：上皮维持其建筑完整性，并且没有中断性核迁移的中断，尊重细胞周期阶段。此外，普遍低的增生率没有增加。也就是说，这些培养条件似乎具有针对PVE细胞增殖的相对选择性的相对选择性的机制。事实上，这种作用受FGF-2或1-辛醇调节的机制显然在发育中受到了调节，仅在其中表达神经遗传学梯度的相对先进区域。