Activation mechanism of lectin-induced response of lymphocytes by polycationic and polyanionic compounds.

聚阳离子和聚阴离子化合物凝集素诱导的淋巴细胞反应的激活机制。

基本信息

批准号：
02660088
负责人：
TAKAHASHI Takao
金额：
$ 1.66万
依托单位：
Mie University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1992
项目状态：
已结题

项目摘要

It is known that the surface charge of lymphocytes is of importance for their mitogen-induced transformation. This was suggested from the fact that the treatment of rat lymph-node lymphocytes with neuraminidase or the addition of polycations, poly -L-ornithine or poly-D-lysine, enhanced their response to mitogens. Other experiments by previous workers showed that some glycoproteins found in normal serum interfered with the in vitro response of lymphocytes to mitogens. One characteristic of these glycoproteins such as alpha-glycoproteins is a relatively high sialic acid content. Larsen and Heron reported that the mitogen-induced response of human lymphocytes was enhanced by such polycationic compounds as poly-D-lysine, DEAE-dextran, protamin sulfate and methylated serum albumin, whereas it was inhibited by the polyanionic compounds, ceruloplasmin and chondroitin sulfate. I report the effects of chondroitin sulfate A on the lymphocyte mitogenesis induced by concanavalin A. A low concentration of chondroitin sulfate enhanced a suboptimal dose of concanavalin A -induced response of lymphocytes. Chondroitin sulfate increased the incorporation of ^3H-thymidine and the number of blast cells in lymphocyte mitogenesis induced by a suboptimal dose of concanavalin A. Chondroitin sulfate increased the viability and the cell surface charge of lymphocytes in the absence of concanavalin A, but it did not affect the concanavalin A -induced Ca^<2+> influx into lymphocytes.

众所周知，淋巴细胞的表面电荷对于有丝分裂原诱导的转化非常重要。这是因为用神经氨酸酶处理大鼠淋巴结淋巴细胞或添加聚阳离子、聚-L-鸟氨酸或聚-D-赖氨酸增强了它们对有丝分裂原的反应。先前工作人员的其他实验表明，正常血清中发现的一些糖蛋白会干扰淋巴细胞对有丝分裂原的体外反应。这些糖蛋白例如α-糖蛋白的一个特征是相对较高的唾液酸含量。 Larsen和Heron报道，聚D-赖氨酸、DEAE-葡聚糖、硫酸鱼精蛋白和甲基化血清白蛋白等聚阳离子化合物可增强人淋巴细胞有丝分裂原诱导的反应，而聚阴离子化合物、铜蓝蛋白和硫酸软骨素则可抑制该反应。。我报告了硫酸软骨素 A 对刀豆球蛋白 A 诱导的淋巴细胞有丝分裂的影响。低浓度的硫酸软骨素增强了刀豆球蛋白 A 诱导的次优剂量的淋巴细胞反应。硫酸软骨素增加了 ^3H-胸苷的掺入以及由次优剂量的伴刀豆球蛋白 A 诱导的淋巴细胞有丝分裂中母细胞的数量。在没有伴刀豆球蛋白 A 的情况下，硫酸软骨素增加了淋巴细胞的活力和细胞表面电荷，但它确实增加了淋巴细胞的有丝分裂。不影响伴刀豆球蛋白A诱导的Ca 2+ 流入淋巴细胞。