CORTICAL DEVELOPMENT

皮质发育

• 批准号: 3394673
• 负责人: VERNE S CAVINESS
• 金额: $ 19.89万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-09-26 至 1994-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3394673
• 关键词: autoradiography; biological models; brain; bromodeoxyuridine; cell cell interaction; cell cycle; cell differentiation; cell growth regulation; cell migration; cell population study; cerebral cortex; developmental neurobiology; embryo /fetus; glia; histogenesis; histology; infant animal; laboratory mouse; monoclonal antibody; neurogenesis; radiotracer; thymidine; tritium

DESCRIPTION: (Investigator's Abstract): The proposed research will determine the patterns of cell proliferation, migration and dispersion of a defined region of the developing murine cerebral cortex, the dorsomedial sector of the E13-E17 cerebral wall. Patterns of cell generation will be determined for the total population of the VZ and SVZ by the method of cumulative labeling with BUdR. Patterns of generation of the "indifferent" population as well as cells of the astroglial lineage will be discriminated by staining with the cellular antigen marker for radial glial cells, RC2. Parameters of cell production will be based upon the output pattern of a 1 hour cohort of S-phase cells, identified by 3H-thymidine pulse labeling, and followed in 1 hour by a cumulative labeling with BUdR to reveal the total population. The patterns of clonal output and of clonal dispersion will be corroborated by labeling with retroviral transfer of xgal at E12.

描述：（研究者的摘要）：拟议的研究将 确定细胞增殖，迁移和分散的模式 发育中的鼠大脑皮层的定义区域，背部 E13-E17大脑壁的部门。 细胞生成的模式将是 通过通过方法确定VZ和SVZ的总人口 用budr累积标记。 “冷漠”产生的模式 人口和星形胶质血统的细胞将被歧视 通过用径向神经胶质细胞的细胞抗原标记染色RC2。 细胞生产的参数将基于1的输出模式 S期细胞的小时队列，通过3H-胸腺定脉冲标记鉴定， 然后在1小时内与Budr进行累积标签，以揭示 总人口。 克隆输出和克隆分散的模式 将通过在E12处用XGAL的逆转录病毒转移来证实。