Role of cAMP, cGMP and Ca++ in chemotaxis of Schistosome Miracidia

cAMP、cGMP 和 Ca 在血吸虫 Miracidia 趋化中的作用

基本信息

批准号：
11670244
负责人：
AOKI Yoshiki
金额：
$ 2.43万
依托单位：
Nagasaki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

项目摘要

Schistosoma miracidia show chemotaxis, exactly speaking klinokinesis, towards the excretion/secretion of the intermediate snails. The present study was attempted to examine the signal transduction of miradial behavior changes caused by the excretion/secretion of the snails and examine the demographic character of snails which secrete the attractant for miracidia.1. Signal transduction of chemotaxis of miradicia towards the secretion of snails.The method was developed to observe qualitatively and quantitatively the chemotactic activity of miracidia. Miracidia were allowed to swim free in a rectangular glass tube. An aliquot of 0.5 ul of secretion of snails was syringe inoculated at the end of tube, then the behavior changes of miracidia was recorded on the video tape. The experiments were attempted to examine pharmacologically the factors which are involved in the behavior changes of miracidia caused by the secretion of snails. The membrane permeable |cAMP and IBMX induced the behavior changes which mimic those caused by the secretion of snails. Non-membrane permeable CAMP and CGMP did not cause any change. Serotonin induced the behavior changes. Miracidia exposed to eserine did not show the chemotaxis towards the secretion of snails. These results suggest that CAMP is involved in the chemotaxis of miracidia towards the snails and the behavior changes are induced by the contraction of muscles.2. Demographic character of snails which secrete thp attractant for miracidia.The experiment was attempted to examine the aciivity of secretion of snails with different ages or with/without infection, and of different species of snailak Schistosome intermediate hosts secrete the attractant for miracidia regardless of age and infection by schistosome. Out of the snails which are not intermediate hosts, some snails secrete powerful attractant for schistosojoie.

奇迹症的血吸虫表现出趋化性，正好讲klinokinesis，用于排泄/分泌中间蜗牛。本研究试图检查由蜗牛的排泄/分泌引起的奇迹行为变化的信号转导，并检查蜗牛的人口统计学特征，这些蜗牛分泌了奇迹症1。 Miradicia向蜗牛分泌的趋化性的信号转导。该方法是为了在定性和定量上观察到奇迹症的趋化活性。允许奇迹在矩形玻璃管中游泳。在试管末端接种了0.5 ul蜗牛分泌的等分试样，然后在录像带上记录了奇迹的行为变化。试图从药理学上检查实验，这些因素与蜗牛分泌引起的奇迹变化有关。膜可渗透| CAMP和IBMX引起了模仿蜗牛分泌引起的行为变化。非膜可渗透的营地和CGMP不会引起任何变化。 5-羟色胺诱导行为变化。暴露于埃瑟琳的奇迹并未显示出对蜗牛分泌的趋化性。这些结果表明，cAMP参与了奇迹对蜗牛的趋化性，而行为的变化是由肌肉收缩引起的。2。蜗牛的人口统计学特征是分泌奇迹的吸引者。该实验试图检查具有不同年龄或/没有感染的蜗牛的分泌的敏捷性，以及不同种类的Snailak schistosom中间宿主分泌的蜗牛，分泌了奇迹症的吸引力，无论是通过schistosome而言，奇迹和感染。在不是中间宿主的蜗牛中，一些蜗牛为Schistosojoie分泌了强大的吸引力。