Control of Ciliary Beating of Schistosomal Miracidia

血吸虫纤毛跳动的控制

• 批准号: 09670266
• 负责人: AOKI Yoshiki
• 金额: $ 1.73万
• 依托单位: NaGASAKI UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670266/
• 关键词: schistosome; miracidium; cilia; cAMP; potassium ion channel; protein k-inase A; chemotaxis; osmotic pressure; cGMP; イオンチャネル; 膜電位

Schistosomal miracidia are completely covered by numerous cilia. The vigorous beating of cilia of miracidia is the driving force of hatching of the egg, swimming in water and invasion into the intermediate snail. The present study aimed at examination of the control mechanism of ciliary beating of miracidia of Schistosoma manosni.Although some external stimulus cause the change in the ciliary beating, the present study dealt with the control mechanism of ciliary beating by osmotic pressure and possible change of ciliaty beating in chemotactic swimming activity.The highlights of the results are 1) that hatching of the eggs is caused by the combination of vigorous beating of cilia and swelling of eggshell, 2) that membrane permeable cAMP, forskokin and IBMX cause the ciliary beating of miracidia which remain to he quiet under high osmotic pressure. 3) that protein kinase A inhibitor and potassium ion channel blocker suppress the ciliary beating of miracidia which swim in water, 4) that membrane permeable cAMP, but not non-permeable cAMP, cause miracidia to take peculiar swimming activity which mimic the chemotactic (klinokinetic) activity of miracidia to snail conditioned water (SCW), and 5) that chemotactic activity of miracidia to SCW is inhibited by eserine.Our results propose the possible control mechanism of ciliary beating of miracidia. Osmotic receptors are present over the integument of miracidia. When the receptors are activated by low osmotic pressure, potassium ion channel is activated. Influx of potassium ions activates adenyl cyclase. Then increase in cAMP concentration activates microtuble movement, then ciliary heating starts. When miracidia are exposed to high osmotic pressure, potassium ion channels are inactivated. Decrease in cAMP concentration suppress the ciliary beating. Chemotactic activity is not controlled by ciliary beating.

血吸虫奇迹完全被许多纤毛覆盖。奇迹的纤毛剧烈跳动是孵化卵，在水中游泳并入侵中间蜗牛的动力。本研究旨在检查马诺斯尼菌奇迹般奇迹的控制机制。尽管某些外部刺激会导致纤毛跳动的变化，但本研究涉及通过渗透压的控制机制，通过肌电压力的控制机制，并可能在肌动症的游泳活动中造成的蛋白质的蛋白质。 2）膜可渗透的营地，福斯科金和IBMX的纤毛和肿胀，导致奇迹般的纤毛跳动，这在高渗透压下保持安静。 3）蛋白激酶一种抑制剂和钾离子通道阻滞剂抑制了在水中游泳的奇迹般的纤毛跳动，4）该膜可渗透的营地，但不可渗透的营地，但不可渗透的营地，导致奇迹引起奇特的游泳活动，从而模仿趋化（klinokinokinokinokinokinetia and snail snail snail and snail sewail）的活动（sc）埃塞琳（Eserine）抑制了奇迹到SCW。巨虫受体存在于奇迹的外皮上。当受体被低渗透压激活时，会激活钾离子通道。钾离子的流入激活腺基环化酶。然后增加cAMP浓度会激活微型运动，然后开始睫状加热。当奇迹暴露于高渗透压时，钾离子通道被灭活。训练营浓度的降低抑制了睫毛跳动。趋化活性不受睫状跳动的控制。