The mechanism of pregnancy from the viewpoint of mutual control system of mother and embryo

从母体与胚胎相互控制系统看妊娠机制

基本信息

批准号：
11307031
负责人：
TAKETANI Yuji
金额：
$ 17.52万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

项目摘要

For the breakthrough of reproductive medicine, the comprehensive understanding on the implantation mechanism is indispensable. In this study, we focused on mutual control system of embryo and mother in the implantation phenomenon, and intended to grasp the dynamic mechanism of implantation polyphyletically.To begin with, we examined how the peripheral blood lymphocyte and the decidual lymphocyte in the implantation site respond to a membrane bound HLA- G antigen on the villus. It was demonstrated that the secretion of the Th1 cytokine is decreased when the peripheral blood lymphocyte recognizes HLA-G, while the secretion of the Th2 cytokine is increased. On the other hand, for the decidual lymphocyte, the secretion of the Th1 cytokine is stimulated by the contact with HLA-G, while the secretion of the Th2 cytokine does not change. The differential response between the peripheral blood lymphocyte and the decidual lymphocyte may be explained by the fact that the B cell exist in the perip … More heral blood but not in decidua, and the blocking antibody crucial for the maintenance of pregnancy can be produced only by the peripheral B cell.Next, we investigated the expression of angiogenin, the potent angiogenic substance, in the human ovaries and its regulatory mechanisms. Angiogenin was shown to be present in follicular fluid and granulosa cell obtained from the patient for in vitro fertilization and embryo transfer. In the granulosa cell culture, the secretion of angiogenin was stimulated 3 times by hCG or CAMP, 5.5 times by the hypoxia stimulation. The mRNA level of angiogenin was 12.5 times up regulated by the hypoxia stimulation.In addition, we attempted to elucidate the physiological roles of cholesterol sulfate, which increased in the uterine endometrium at the implantation period. As a result, it was shown that cholesterol sulfate inhibited the invasion of trophoblast into the endometrium, and the mechanism of the inhibition was non-competitive inhibition of the plasmin activity. Less

对于繁殖医学的突破，对植入机制的全面理解是必不可少的。 In this study, we focused on mutual control system of embryo and mother in the implantation phenomenon, and intended to grap the dynamic mechanism of implantation polyphyleically.To begin with, we examined how the peripheral blood lymphocyte and the decidual lymphocyte in the implantation site responded to a membrane bound HLA- G antigen on the villus.证明当周围血液淋巴细胞识别HLA-G时，Th1细胞因子的分泌会进展，而Th2细胞因子的分泌增加。另一方面，对于Decidel淋巴细胞，与HLA-G的接触刺激Th1细胞因子的分泌，而Th2细胞因子的分泌不会改变。周围血液淋巴细胞与decIDEL淋巴细胞之间的差异反应可以通过以下事实来解释：B细胞存在于Perip中的B细胞……更多的先驱血液，但在决定性抗体中却不是在Chinceua中，而对维持怀孕至关重要的阻塞抗体只能由Angient an angient angigent angigent angient ongogentions Angient，我们才能产生。及其监管机制。血管生成蛋白显示出存在于卵泡液中，并从患者获得体外受精和胚胎转移的颗粒细胞中。细胞培养，HCG或CAMP刺激了血管葡萄蛋白的分泌3次，低氧刺激刺激了5.5倍。血管生成蛋白的mRNA水平受到缺氧刺激调节的12.5倍。此外，我们试图阐明硫酸胆固醇的物理作用，硫酸胆固醇的物理作用在植入期间在子宫内膜中增加。结果，结果表明硫酸胆固醇抑制了滋养细胞进入子宫内膜的侵袭，抑制作用的机理是对纤溶酶活性的非竞争性抑制。较少的