Study on the Role of Prolactin in Reproductive Physiology.

催乳素在生殖生理学中作用的研究。

基本信息

批准号：
62570744
负责人：
TAKETANI Yuji
金额：
$ 1.15万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1989
项目状态：
已结题

项目摘要

Modulatory effects of prolactin(PRL) on gonadotropin release were examined. An acute administration of OPRL to ovariectomized rats induced no influence on the content of gonadotropin-releasing hormone(GnRH) in the hypothalamus. However, the release of GnRH and gonadotropin were inhibited in rats given OPRL. The reduction in the gonadotropin release seems to be ascribable to the suppression of GnRH release. An acute elevation in PRL levels did not affect the pituitary responsiveness to GnRH. Thus, we deduced from these results that hyperprolactinemia inhibits the release of gonadotropin by primarily impairing the release of GnRH from the hypothalamus.Then, the action of PRL on the secretion of donadotropin was investigated using a primary cell culture system of rat anterior pituitary gland. LH release was increased by adding PRL into culture media in a dose-dependent manner in the range between 10-1000 ng/ml. The stimulatory effect of PRL was further augmented in the presence of GnRH an … More d estradiol. PRL also caused an increase in FSH release. These data demonstrate that PRL stimulates the release of gonadotropin at the pituitary level, suggesting a paracrine mode of action of PRL in the anterior pituitary gland.Clinically, the synchronous secretion of LH and PRL is frequently observed in normal cycling and hypogonadal women. Looking for the effects of GnRH on the PRL release, GnRH was given to women with normal menstruation and various menstrual disorders. This study offered such conclusion that GnRH occasionally alters the blood levels of PRL in either positive or negative manner depending on basal PRL levels.In order seek the underlying mechanism of infertility associated with endometriosis, we demonstrate a close linkage of endometriosis and inadequate luteal function as evidenced by lower levels of progesterone at midluteal phase. There appears to be no correlation between the levels of progesterone and PRL. Therefore, conclusion is drawn that an aberrant secretion of PRL has no relation to the impaired corpus luteum function in endometriosis. Less

研究了催乳素（PRL）对促性腺激素释放的调节作用，对切除卵巢的大鼠进行急性给药，对下丘脑促性腺激素释放激素（GnRH）的含量没有影响，但GnRH和促性腺激素的释放受到抑制。给予 OPRL 的大鼠促性腺激素释放的减少似乎归因于 GnRH 释放的急剧升高。 PRL 水平不影响垂体对 GnRH 的反应，因此，我们从这些结果推断，高催乳素血症主要通过损害下丘脑 GnRH 的释放来抑制促性腺激素的释放。然后，使用 PRL 对促性腺激素分泌的作用进行了研究。大鼠垂体前叶原代细胞培养系统通过在培养基中添加PRL以剂量依赖性方式增加LH释放。 10-1000 ng/ml PRL 的刺激作用在 GnRH 和 PRL 存在下进一步增强，这些数据表明 PRL 刺激垂体水平的促性腺激素释放。提示 PRL 在垂体前叶中的旁分泌作用模式。临床上，在正常循环和正常循环中经常观察到 LH 和 PRL 的同步分泌。性腺功能减退的女性。为了寻找 GnRH 对 PRL 释放的影响，GnRH 被给予正常月经和各种月经失调的女性，该研究得出这样的结论：GnRH 偶尔会根据基础值以正向或负向的方式改变 PRL 的血液水平。 PRL 水平。为了寻找与子宫内膜异位症相关的不孕症的根本机制，我们证明了子宫内膜异位症和黄体功能不足之间的密切联系，这一点可以通过孕酮水平较低来证明。黄体中期，孕激素和 PRL 水平之间似乎没有相关性，因此得出结论：子宫内膜异位症中 PRL 的异常分泌与黄体功能受损无关。