Study on the Role of Prolactin in Reproductive Physiology.

催乳素在生殖生理学中作用的研究。

基本信息

批准号：
62570744
负责人：
TAKETANI Yuji
金额：
$ 1.15万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1989
项目状态：
已结题

项目摘要

Modulatory effects of prolactin(PRL) on gonadotropin release were examined. An acute administration of OPRL to ovariectomized rats induced no influence on the content of gonadotropin-releasing hormone(GnRH) in the hypothalamus. However, the release of GnRH and gonadotropin were inhibited in rats given OPRL. The reduction in the gonadotropin release seems to be ascribable to the suppression of GnRH release. An acute elevation in PRL levels did not affect the pituitary responsiveness to GnRH. Thus, we deduced from these results that hyperprolactinemia inhibits the release of gonadotropin by primarily impairing the release of GnRH from the hypothalamus.Then, the action of PRL on the secretion of donadotropin was investigated using a primary cell culture system of rat anterior pituitary gland. LH release was increased by adding PRL into culture media in a dose-dependent manner in the range between 10-1000 ng/ml. The stimulatory effect of PRL was further augmented in the presence of GnRH an … More d estradiol. PRL also caused an increase in FSH release. These data demonstrate that PRL stimulates the release of gonadotropin at the pituitary level, suggesting a paracrine mode of action of PRL in the anterior pituitary gland.Clinically, the synchronous secretion of LH and PRL is frequently observed in normal cycling and hypogonadal women. Looking for the effects of GnRH on the PRL release, GnRH was given to women with normal menstruation and various menstrual disorders. This study offered such conclusion that GnRH occasionally alters the blood levels of PRL in either positive or negative manner depending on basal PRL levels.In order seek the underlying mechanism of infertility associated with endometriosis, we demonstrate a close linkage of endometriosis and inadequate luteal function as evidenced by lower levels of progesterone at midluteal phase. There appears to be no correlation between the levels of progesterone and PRL. Therefore, conclusion is drawn that an aberrant secretion of PRL has no relation to the impaired corpus luteum function in endometriosis. Less

检查了催乳素（PRL）对促性腺激素释放的调节作用。 OPRL对卵巢切除的大鼠的急性给药不会引起对下丘脑中促性腺激素释放雌酮（GNRH）含量的影响。然而，在给定的OPRL的大鼠中抑制了GnRH和促性腺激素的释放。促性腺激素释放的减少似乎归因于GNRH释放的抑制。 PRL水平的急性升高不会影响垂体对GNRH的反应性。这是我们从这些结果中得出的，高溶式血症血症抑制了促性腺激素的释放，从而损害了从下丘脑中释放GNRH的释放。然后，使用大鼠抗垂体的原代细胞培养系统研究了PRL对唐纳多尔蛋白分泌的作用。通过在10-1000 ng/mL之间以剂量依赖性方式将PRL添加到培养基中来增加LH释放。在GNRH存在的情况下，PRL的刺激作用进一步增强。 PRL还导致FSH释放增加。这些数据表明，PRL刺激垂体水平的促性腺激素的释放，表明PRL在垂体前腺体中的旁分泌作用方式。在正常的循环和低辅助女性中经常观察到LH和PRL的同步分泌。寻找GNRH对PRL释放的影响，GNRH是对正常月经疾病的女性的。这项研究得出了这样的结论，即GNRH偶尔会根据基本PRL水平以阳性或负面的方式改变PRL的血液水平。在命令中寻求与子宫内膜异位相关的不育的潜在机制，我们证明了子宫内膜异位症的紧密联系，并在中性较低阶段的较低水平上证明了子宫内膜异位症和不足的鲁uteal功能。孕酮和PRL的水平之间似乎没有相关性。因此，得出结论是，PRL的异常分泌与子宫内膜异位症中的Luteum功能没有关系。较少的