A Study of Interaction between Egg and Endometrium in Implantation.

着床时卵子与子宫内膜相互作用的研究。

• 批准号: 02454377
• 负责人: TAKETANI Yuji
• 金额: $ 4.22万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-02454377/
• 关键词: implantation; epidermal growth factor; endometrium; prostaglandin; tissue-plasminogen activator; EGF receptor; folliculogenesis; embryogenesis; activin; follistatin; epidermal growth factor; follicle; granulosa cells; FSH; CAMP; progesteron

The present study was undertaken to investigate the mechanism of implantation taking the embryo-maternal interaction into account. First of all, epidermal growth factor (EGF) -like immunoreactivity was demonstrated in human endometrium along with the receptor for EGF.In primary cell culture of human endometrial cells, EGF stimulated the release of both prostaglandin and tissue-plasminogen activator which are supposed to be involved in implantation. EGF was further shown to enhance glycogenolysis so as to supply embryos with glucose requires for nidation. Altogether, EGF is implicated to have a role in implantation. Another finding with EGF is that estradiol increased the amount of EGF receptor in combination with progesterone in human endometrial cells. As for folliculogenesis, EGF stimulated the proliferation of cultured rat granulosa cells in concert with FSH.In view of the presence of EGF-like substance in the ovaries, EGF is assumed to regulate folliculogenesis. In addition, EGF stimulated the development of mouse 2-cell embryo in vitro. Thus, it appears that EGF has diverse biological actions in reproduction, such as folliculogenesis, embryonal development and implantation, etc.

本研究旨在探讨考虑胚胎与母体相互作用的植入机制。首先，在人子宫内膜中证明了表皮生长因子（EGF）样免疫反应性以及 EGF 受体。在人子宫内膜细胞的原代细胞培养物中，EGF 刺激前列腺素和组织纤溶酶原激活剂的释放，这两种物质被认为是参与植入。 EGF 进一步被证明可以增强糖原分解，从而为胚胎提供着床所需的葡萄糖。总而言之，EGF 在着床过程中发挥着重要作用。 EGF 的另一个发现是，雌二醇与黄体酮结合可增加人类子宫内膜细胞中 EGF 受体的数量。在卵泡发生方面，EGF与FSH协同刺激培养的大鼠颗粒细胞的增殖。鉴于卵巢中存在EGF样物质，推测EGF具有调节卵泡发生的作用。此外，EGF在体外刺激小鼠2细胞胚胎的发育。由此可见，EGF在生殖中具有多种生物学作用，如卵泡发生、胚胎发育和着床等。

项目成果

S.Ishihara,Y.Taketani,M.Mizuno: "Endomctricsis impairs glycogen synthesis in human endimetrium." Int.J.Fertili. 36. 287-270 (1991)

S.Ishihara、Y.Taketani、M.Mizuno：“子宫内膜收缩会损害人子宫内膜中的糖原合成。”

T.M.Kuo, Y.Taketani, T.Shimizu, M.Mizuno.: "A possible mechanism of infertility in endometriosis. -A role of interleukin 1 in peritoneal fluid." Assist.Reprod.Reprod.Tech/Androl.1. 24-31 (1990)

T.M.Kuo、Y.Taketani、T.Shimizu、M.Mizuno.：“子宫内膜异位症不孕的可能机制。-白细胞介素 1 在腹膜液中的作用。”

S.Ishihara,Y.Taketani,M.Mizuno.: "Epidermal growth factor-like immunoreactivity in human endometrium." Asia Oceania J.Obstet.Gynecol. 16. 165-168 (1990)

S.Ishihara，Y.Taketani，M.Mizuno.：“人子宫内膜中的表皮生长因子样免疫反应性。”

郭,武谷,綾部,五味淵,矢野,堤,水野: "子宮内膜症腹腔内貯留液のマウス胚発生に及ぼす影響" 日本産婦人科学会誌. 42. 1284-1290 (1990)

Kaku、Takeya、Ayabe、Gomifuchi、Yano、Ttsutsumi、Mizuno：“子宫内膜异位腹膜内液对小鼠胚胎发育的影响”日本妇产科学会杂志 42. 1284-1290 (1990)。

Y.Taketani.T.M.Kuo M.Mizuno: "Comparison of cytokine levels and embryo toxicity in peritoneal fluid in infertile women with untreated or treated endometriosis" Am.J.Obstet.Gynecol.167. 265-270 (1992)

Y.Taketani.T.M.Kuo M.Mizuno：“未治疗或已治疗子宫内膜异位症的不孕妇女腹腔液中细胞因子水平和胚胎毒性的比较”Am.J.Obstet.Gynecol.167。