Analysis of Angiogenesis for the Intra-Articular Lesions of Temporomandibular Joint Disorders by Mechanical Stress

机械应力引起的颞下颌关节疾病关节内病变的血管生成分析

• 批准号: 10671773
• 负责人: SATO Tsuyoshi
• 金额: $ 1.92万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671773/
• 关键词: Temporomandibular Joint Disorders; Joint Pain; Posterior Disk Attachment; MR Images; Angiogenesis; Estrogen; Cytokine; Nitric Oxide; TMJ

Analysis of Intra-Articular Inflammatory changes for Temporomandibular Joint DisordersThe present study evaluated the relationship between joint pain, contrast enhancement of the posterior disk attachment by MR imaging, and nitric oxide production in the synovial fluid of patients with TMJ disorders.Subjective pain level and location were determined by the patient's self assessment using the 100 mm visual analog scale. Synovial fluid was collected from the superior compartment of the joint using a direct aspiration technique. The level of nitric oxide production in the synovial fluid was measured by the Griess reaction.The degree of pain was correlated with contrast enhancement of the posterior disk attachment (n=40, r=0.70, P<0.001). The elevated level of nitric oxide in synovial fluid was associated with high degree of joint pain and prominent enhancement of the posterior disk attachment. Nitric oxide may be a critical mediator that functions to clarify the inflammatory cascade in the TMJ region.Effects or Estrogen on cytokine Secretion and Nitric Oxide production from Human Synovial FibroblastsIn the present study, we investigated the molecularbiological effects of estrogen on inflammatory cytokine production, nitric oxide production, and iNOS mRNA expression using the synovial tissues of femoral joint from a patient with rheumatoid arthritis.17b-estradiol significantly inhibited GM-CSF secretion and nitrite production in the culture medium in a concentration-dependent manner which reached significance at 10 mM (p<0.05). Using RT-PCR techniques the expression of the iNOS mRNA could be demonstrated in unstimulated and cytokine-stimulated cultures of synovial fibroblasts in the presence of estradiol (10 mM). Our results suggest that synovial fibroblasts are sensitive to the influence of estrogen and provide information for their role as immunomodulatory factors. Estrogen indeed might exert protective effects in the inflamed joint.

颞下颌关节疾病的关节内炎症变化分析本研究评估了关节疼痛、磁共振成像后椎间盘附件的对比增强以及颞下颌关节疾病患者滑液中一氧化氮的产生之间的关系。主观疼痛程度和部位由患者使用 100 毫米视觉模拟量表进行自我评估来确定。使用直接抽吸技术从关节的上室收集滑液。通过格里斯反应测量滑液中一氧化氮的产生水平。疼痛程度与后椎间盘附件的对比增强相关（n=40，r=0.70，P<0.001）。滑液中一氧化氮水平升高与严重的关节疼痛和后椎间盘附着的显着增强有关。一氧化氮可能是澄清 TMJ 区域炎症级联反应的关键介质。雌激素对人滑膜成纤维细胞细胞因子分泌和一氧化氮产生的影响在本研究中，我们研究了雌激素对炎症细胞因子产生、一氧化氮的分子生物学影响。使用类风湿性关节炎患者的股骨关节滑膜组织观察氧化物的产生和 iNOS mRNA 的表达。17b-雌二醇显着以浓度依赖性方式抑制培养基中 GM-CSF 的分泌和亚硝酸盐的产生，在 10 mM 时达到显着性（p<0.05）。使用 RT-PCR 技术，在雌二醇 (10 mM) 存在的情况下，可以在未刺激和细胞因子刺激的滑膜成纤维细胞培养物中证明 iNOS mRNA 的表达。我们的结果表明滑膜成纤维细胞对雌激素的影响敏感，并为其作为免疫调节因子的作用提供了信息。雌激素确实可能对发炎的关节发挥保护作用。