Analysis of Angiogenesis for the Intra-Articular Lesions of Temporomandibular Joint Disorders by Mechanical Stress

机械应力引起的颞下颌关节疾病关节内病变的血管生成分析

• 批准号: 10671773
• 负责人: SATO Tsuyoshi
• 金额: $ 1.92万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671773/
• 关键词: Temporomandibular Joint Disorders; Joint Pain; Posterior Disk Attachment; MR Images; Angiogenesis; Estrogen; Cytokine; Nitric Oxide; TMJ

Analysis of Intra-Articular Inflammatory changes for Temporomandibular Joint DisordersThe present study evaluated the relationship between joint pain, contrast enhancement of the posterior disk attachment by MR imaging, and nitric oxide production in the synovial fluid of patients with TMJ disorders.Subjective pain level and location were determined by the patient's self assessment using the 100 mm visual analog scale. Synovial fluid was collected from the superior compartment of the joint using a direct aspiration technique. The level of nitric oxide production in the synovial fluid was measured by the Griess reaction.The degree of pain was correlated with contrast enhancement of the posterior disk attachment (n=40, r=0.70, P<0.001). The elevated level of nitric oxide in synovial fluid was associated with high degree of joint pain and prominent enhancement of the posterior disk attachment. Nitric oxide may be a critical mediator that functions to clarify the inflammatory cascade in the TMJ region.Effects or Estrogen on cytokine Secretion and Nitric Oxide production from Human Synovial FibroblastsIn the present study, we investigated the molecularbiological effects of estrogen on inflammatory cytokine production, nitric oxide production, and iNOS mRNA expression using the synovial tissues of femoral joint from a patient with rheumatoid arthritis.17b-estradiol significantly inhibited GM-CSF secretion and nitrite production in the culture medium in a concentration-dependent manner which reached significance at 10 mM (p<0.05). Using RT-PCR techniques the expression of the iNOS mRNA could be demonstrated in unstimulated and cytokine-stimulated cultures of synovial fibroblasts in the presence of estradiol (10 mM). Our results suggest that synovial fibroblasts are sensitive to the influence of estrogen and provide information for their role as immunomodulatory factors. Estrogen indeed might exert protective effects in the inflamed joint.

分析颞下颌关节疾病的关节内炎症变化本研究评估了关节疼痛，MR成像通过MR成像对后椎间盘附着的对比度增强的对比度增强，以及使用患者的自我评估量表来确定TMJ疾病患者的滑液中的一氧化氧化物的产生。使用直接抽吸技术从关节的上室收集滑液。滑液中的一氧化氮产生水平是通过Griess反应测量的。滑液中一氧化氮水平升高与高度关节疼痛和后盘附着的显着增强有关。 Nitric oxide may be a critical mediator that functions to clarify the inflammatory cascade in the TMJ region.Effects or Estrogen on cytokine Secretion and Nitric Oxide production from Human Synovial FibroblastsIn the present study, we investigated the molecularbiological effects of estrogen on inflammatory cytokine production, nitric oxide production, and iNOS mRNA expression using the synovial tissues of femoral joint 17b-雌二醇从患有类风湿关节炎的患者中显着抑制GM-CSF的分泌和亚硝酸盐在培养基中的浓度依赖性方式，在10 mm处达到显着性（p <0.05）。使用RT-PCR技术，可以在雌二醇（10 mM）存在下未刺激和细胞因子刺激的滑膜成纤维细胞的培养物中证明iNOS mRNA的表达。我们的结果表明，滑膜成纤维细胞对雌激素的影响敏感，并为其作为免疫调节因素的作用提供信息。雌激素确实可能在发炎的关节中发挥保护作用。