Lymphoscintigraphy with radio-colloidal agent

使用放射性胶体剂进行淋巴闪烁扫描

• 批准号: 17591970
• 负责人: SATO Tsuyoshi
• 金额: $ 2.24万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591970/
• 关键词: lymph node; scintigram; radio-colloid; PET; 悪性腫瘍

(Materials ＆ Methods) Patients with oral cancer were examined by the scintigraphy with Tc-radio-colloid and PET with FDG. To detect lymph node metastasis, lymphoscintigraphy and PET scan were performed. In lymphoscintigraphy, two areas of injection were selected. One is the extra-oral subcutaneous area behind the ear for the cervical lymph nodes. The other is the intra-oral sub mucosal area of mucobuccal fold of the upper molar region for the submandibular lymph nodes. The radio-colloid in each volume of 0.25 ml (37MBq) was injected in both sides of each area respectively. Scanning was done immediately after injection with Gamma camera. In PET, FDG of 3.7MBq/kg was injected intravenously and the scanning was done after 40 min from the injection. Furthermore, the tumor samples, obtained either after the biopsy or after the operation, were embedded in paraffin wax and cut into thin sections for the immunohistochemical staining of the glucose transpototer. The accumulation of the lymph no … More des was evaluated into three groups and in PET scanning was evaluated into three grades respectively. The immunohistochemical staining was also evaluated into three grades visually. (Results) In PET scanning, around 75% of metastatic lymph nodes were detected. But, the detection strongly depended upon the size of lymph nodes and small lymph nodes were hardly detected (under 5%). The SUV ranged from 2 to 6 among lymph nodes and distributed widely. In lymphoscintigraphy, metastatic lymph nodes showed three images of defect, mottled and rope-like appearances. These images showed a close correlation with the pathologic manifestations respectively. In immunohistochemical staining of the glucose transporter, all metastatic lymph nodes showed from a moderate to a severe staining. (Conclusions) Lymphoscintigraphy with radio-colloida: agent and PET scanning with FDG were useful for the detection of metastatic lymph nodes of relatively large size. However, these methods were helpless for the small metastatic lymph nodes. Therefore, we will make a further evaluation to establish a technique for the detection of the small metastatic lymph nodes. Less

(材料和方法) 对口腔癌患者进行Tc放射性胶体闪烁扫描和FDG PET检查。为了检测淋巴结转移，进行了淋巴闪烁扫描和PET扫描。在淋巴闪烁扫描中，选择了两个注射区域。耳后的口外皮下区为颈部淋巴结，另一个是口内上颌粘膜皱襞的粘膜下区。颌下淋巴结的磨牙区两侧分别注射0.25ml（37MBq）的放射性胶体，注射后立即用Gamma相机进行扫描，FDG为3.7MBq/kg。静脉注射，注射40分钟后进行扫描。此外，将活检后或手术后获得的肿瘤样本包埋在石蜡中。蜡并切成薄片进行葡萄糖转运蛋白的免疫组化染色。将淋巴结的积累分为三组，并在PET扫描中分别评估为三个等级。免疫组化染色也通过肉眼评估为三个等级。 （结果）在PET扫描中，大约75%的转移性淋巴结被检测到，但是，检测强烈依赖于淋巴结的大小，并且小淋巴结很难被检测到（SUV范围在5%以下）。淋巴结中分布广泛，分布广泛，在淋巴显像中，转移淋巴结呈缺损、斑驳和绳索状三种图像，这些图像分别与葡萄糖转运蛋白的病理表现密切相关。所有转移淋巴结均显示中度至重度染色（结论）放射性胶体淋巴闪烁显像和 FDG PET 扫描对于检测转移性淋巴结非常有用。然而，这些方法对于较小的转移淋巴结无能为力，因此，我们将进行进一步的评估，以建立一种较小的转移淋巴结的检测技术。