Basic Research of low back pain

腰痛的基础研究

基本信息

批准号：
10671344
负责人：
TAKAHASHI Kazuhisa
金额：
$ 0.58万
依托单位：
Chiba University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671344/
关键词：
Low back pain Fos brain facet joint dorsal root ganglion referred pain dichotomizing axons double labeling 腰椎椎間板交感神経幹

项目摘要

We studied the mechanism of low back pain using rats, and obtained the following results.(1) Fos expression in the brain and spinal cord in condition of low back pain : Acute noxious stimulation delivered to lumbar muscles (deep pain group) and skin (cutaneous pain group) of rats was used to study Fos expression patterns in the brain and spinal cord. In the spinal cord, Fos-immunoreactive (Fos-ir) neurons were not observed in the lamina II after stimulation of low back muscles. In the brain, Fors-ir neurons were observed in significantly greater numbers in the deep somatic pain group in the accumbens, basolateral nucleus of amygdala, paraventricular hypothalamus, and the ventrolateral periaqueductal gray than in the cutaneous pain group.(2) Substance P and Calcitonin Gene-Related Peptide immunoreactive sensory dorsal root ganglion (DRG)neurons innervating the lumbar facet joints in rats : Neurons innervating the L5/6 facet joint were distributed throughout DRGs from L1 to L5 levels. The ratios of SP and CGRP-ir neurons inL1 and L2 DRGs were significantly less than those in L3, L4 or L5 DRGs. In the physiological condition in rats, lower DRG neurons may have a more significant role in pain sensation of the facests than upper DRG neurons.(3) DRG neurons with dichotomizing axons to the facet joint and sciatic nerve : We demonstrated in rats the existence of the sensory neuron with dichotomizing axons both to the facet joint and the sciatic nerve using double-labelling method. This neuron provides a possible neuro-anatomical substratum for referred leg pain in facet joint disease.

我们使用大鼠研究了下腰痛的机制，并获得了以下结果。（1）在腰痛状态下，FOS表达的FOS表达：肌肉肌肉（深痛组）和皮肤（皮肤疼痛组）和大鼠的急性有害刺激用于研究大脑和脊髓索的FOS表达模式。在脊髓中，在刺激下背部肌肉后，在椎板II中未观察到FOS免疫反应（FOS-IR）神经元。在大脑中，在伏伏氏症，杏仁核的基底外侧核，副丘脑下丘脑和腹侧外侧灰灰色的基底疼痛核中观察到fors-ir神经元，比皮肤酸酯p和腹侧外侧的灰色（2）。（DRG）在大鼠中支配腰部关节的神经元：支配L5/6方面关节的神经元在整个DRG中分布在L1到L5水平。 SP和CGRP-IR神经元INL1和L2 DRG的比率明显小于L3，L4或L5 DRG中的比率。在大鼠的生理状况下，下DRG神经元在脸部的疼痛感觉中可能比上DRG神经元具有更重要的作用。（3）（3）对刻布关节和坐骨神经的DRG神经元：我们在大鼠中表现出具有二分轴轴承轴承的感觉均具有二act轴的轴向轴轴，并且在大鼠中证明了双轴均具有dought siatcric anciic n rab sictic n siccer。该神经元提供了一种可能的神经 - 动态基质，用于引用小平面关节疾病的腿部疼痛。