Study of nociceptive transduction pathways derived from the lumbar spine.

来自腰椎的伤害性转导途径的研究。

基本信息

批准号：
14571358
负责人：
TAKAHASHI Kazuhisa
金额：
$ 2.11万
依托单位：
Chiba University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

项目摘要

Previously, it was believed that the lumbar intervertebral disc was innervated segmentally by dorsal root ganglion (DRG) neurons via the sinuvertebral nerves. Using retrograde tracing methods, we clarified that the lower disc (L5-L6) is innervated predominantly by upper (L1 and L2) DRG neurons via the sympathetic trunks. Furthermore, we investigated the expression of various pain-related molecules such as substance P (SP), calcitonin gene-related peptide (CGRP), isolectin B4 (IB4), P2X_3 receptor and using a combination of immunostaining with the retrograde tracing method We found that about half of the neurons innervating the disc were SP-and CGRP-immunoreactive (-ir), whilst, only a small proportion of the DRG neurons innervating the disc were IB4-and P2Xrpositive, indicating that peptidecontaining DRG neurons ate the main subpopulation which transmits and modulates nociceptive information from the disc.Bennett et al. reported that the proportions of CGRP-ir and IB4-binding neurons are different among cutaneous and visceral primary sensory neurons in the rat. They concluded that the proportion of CGRP-ir neurons was higher in visceral afferents, and the proportion of the IB4-binding neurons was lower in visceral afferents than in cutaneous afferents. Thus, the lumbar intervertebral disc had similar neural profiles to that of visceral organs with regard to the proparions of the two types of DRG neurons. However, the proportion of 1B4-biding neurons is dramatically lower in the disc afferents than cutaneous and visceral afferents. Since CGRP-ir and 1B4-binding neurons are thought to be NGF-and GDNF-sensitive neurons respectively, we suppose that nociceptive information from the lumbar intervertebral disc is mainly transmitted and modulated by NGF-sensitive neurons.

以前，人们认为腰椎椎间盘通过背面神经节（DRG）神经元通过Sinuvertebral神经进行了分段。使用逆行示踪方法，我们阐明了下部圆盘（L5-L6）主要由上（L1和L2）DRG神经元通过交感神经树干支配。此外，我们研究了各种与疼痛相关的分子的表达，例如物质P（SP），降钙素基因相关肽（CGRP）（CGRP），分离蛋白B4（IB4），P2X_3受体，以及使用与逆行的Tracing方法结合了一半的神经元，使神经元的一半是c.而仅一小部分的DRG神经元是IB4和P2XRPOSITER，这表明肽固定的DRG神经元吃了主要的亚群，该亚群可以传播和调节来自Disc.Bennett等人的伤害性信息。报道说，大鼠皮肤和内脏的原发性神经元中CGRP-IR和IB4结合神经元的比例不同。他们得出的结论是，内脏传入中CGRP-IR神经元的比例更高，并且内脏传入的IB4结合神经元的比例低于皮肤传入。因此，腰椎椎间盘具有与内脏器官相似的神经特征，就两种类型的DRG神经元的宣传而言。但是，在椎间盘传播物中，1B4-示出神经元的比例大大低于皮肤和内脏传入。由于CGRP-IR和1B4结合神经元被认为分别是NGF和GDNF敏感的神经元，因此我们认为来自腰椎椎间盘的伤害性信息主要由NGF敏感的神经元传播和调节。