RESEACH FOR THE TRANSDIFFERENTIATION OF PANCREATIC CELLS TO HEPATOCYTES

胰腺细胞向肝细胞转移的研究

• 批准号: 10671201
• 负责人: SETOGUCHI Toshiaki
• 金额: $ 1.98万
• 依托单位: Miyazaki Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671201/
• 关键词: COPPER DEFICIENCY; PANCREATIC HEPATOCYTE; CHOLESTEROL METABOLISM

Background & Aims: Hepatocyte-like cells have been observed in the pancreas of the rat. We examined the bile acid biosynthetic function of these cells to determine whether they are real hepatocytes. This study investigated the gene expression of two liver-specific enzymes regulating bile acid biosynthesis (cholesterol 7α-hydroxylase and ΔィイD14ィエD1-3-ketosteroid 5β-reductase) in the hepatocyte-like cells.Methods : Hepatocyte-like cells were induced in the rat pancreas by a copper depletion-repletion regimen. The expression of messenger RNA for cholesterol 7α-hydroxylase and ΔィイD14ィエD1-3-ketosteroid 5β-reductase was detected using the reverse transcriptase polymerase chain reaction. The initial amount of cholesterol 7α-hydroxylase messenger RNA was quantified using the competitive polymerase chain reaction method.Results: Messenger RNA was expressed in the pancreatic hepatocyte-like cells for cholesterol 7α-hydroxylase and ΔィイD14ィエD1 -3-ketosteroid 5β-reductase. A circadian rhythm of cholesterol 7α-hydroxylase messenger RNA expression was identified.Conclusions: This study confirms that the hepatocyte-like cells produce bile acids.

背景和目的：我们在大鼠的胰腺中观察到了肝细胞样细胞，以确定它们是否是真正的肝细胞。本研究研究了两种调节胆汁的肝脏特异性酶的基因表达。肝细胞样中的酸生物合成（胆固醇 7α-羟化酶和 ΔD14-酮类固醇 5β-还原酶）方法：通过铜消耗-补充方案在大鼠胰腺中诱导肝细胞样细胞，使用逆转录酶聚合酶链式反应检测胆固醇7α-羟化酶和ΔD14D1-3-酮类固醇5β-还原酶的信使RNA的表达。使用竞争性聚合酶链式反应方法对胆固醇 7α-羟化酶信使 RNA 的初始量进行定量。结果：胆固醇7α-羟化酶和ΔD14D1 -3-酮类固醇5β-还原酶的信使RNA在胰腺肝细胞样细胞中表达。确定了胆固醇7α-羟化酶信使RNA表达的昼夜节律。结论：本研究证实了肝细胞样细胞中存在胆固醇7α-羟化酶信使RNA。细胞产生胆汁酸。

项目成果

Ando,Y.,...Setoguchi,T.,et al.: "Expression of cholesterol 7α-hydroxylase and Δ ^4-3-ketosteroid 5β-reductase genes in rat pancreatic hepatocyte-like cells."Journal of Lipid Research. 40. 1793-1798 (1999)

Ando, Y.,...Setoguchi, T., et al.：“大鼠胰腺肝细胞样细胞中胆固醇 7α-羟化酶和 Δ^4-3-酮类固醇 5β-还原酶基因的表达。”脂质杂志研究。40。1793-1798（1999）

Ando,Y., Ide,H., Kosai,S., Kamimura,R., Maeda,Y., Higashi,S., and Setoguchi,T.: "Expression of cholesterol 7α-hydroxylase and ΔィイD14ィエD1-3-ketosteroid 5β-reductase genes in rat pancreatic hepatocyte-like cells"Journal of Lipid Research. 40. 1793-1798 (199

Ando, Y.、Ide, H.、Kosai, S.、Kamimura, R.、Maeda, Y.、Higashi, S. 和 Setoguchi, T.：“胆固醇 7α-羟化酶和 ΔD14 D1-3 的表达-大鼠胰腺肝细胞样细胞中的酮类固醇5β-还原酶基因”脂质研究杂志。40。1793-1798（199）