Bioinorganic chemical studies on metal superoxide dismutases and their behavior in brain by the use of multielemental analysis.

利用多元素分析对金属超氧化物歧化酶及其在大脑中的行为进行生物无机化学研究。

基本信息

批准号：
10640539
负责人：
AMANO Ryohei
金额：
$ 1.98万
依托单位：
KANAZAWA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

项目摘要

This study was undertaken in order to acquire better understanding of the biobehavior metal superoxide dismutases by the techniques of multitracer and instrumental neutron activation analysis. The followings were found.1) The radioactive multitracer technique was applied to a study on the uptake behavior of trace elements in normal C57BL/6N mice. Comparative uptake behavior of ^<46>Sc, ^<54>Mn, ^<59>Fe, ^<58>Co, ^<65>Zn, ^<75>Se, ^<83>Rb and ^<88>Zr tracers was examined among 11 organs (brain, cardiac muscle, lung, liver, spleen, pancreas, kidneys, bone, muscle, eyeballs and testes) and blood, and evaluated in terms of the "tissue uptake rate (the radioactivity percentage of injected dose per gram of tissue, %dose/g)". The multitracer technique revealed reliable data demonstrating characteristic uptake of the 8 trace elements, Sc, Mn, Fe, Co, Zn, Se, Rb and Zn by the brain and other organs, as well as the distinctive feature of the accumulation and retention of each element in the brai … More n. According to these results, it is evident that the metal ions examined in this work are easily transported into the brain, in addition, the high retention of Se, Rb, Mn and Zn in the brain during a fairly long time suggests active involvement of these elements in the brain function.2) Brain regional uptakes for ^<46>Sc, ^<54>Mn, ^<58>Co, ^<65>Zn, ^<75>Se, and ^<83>Rb in mice were examined using multitracer under deficient and excessive states of an essential trace elements, manganese, zinc or selenium. Zn-deficient and excessive states, and Se-deficient and excessive states influence the brain regional uptake behaviors of other trace elements except respective elements. On the other hand, in the mouse brain under Mn-deficient and excessive states, few interrelation between Mn and other elements was found from 0.4ppm to 300.4ppm. Multitracer technique was found to be very useful to screen the element-element interrelation in brain.3) Concentrations and uptake behavior of manganese (Mn), zinc (Zn) and selenium (Se) in mouse brain were studied by means of multitracer technique, neutron activation analysis and autoradiography. Comparative concentrations of Mn, Zn and Se and tracer uptake behavior of ^<54>Mn, ^<65>Zn and ^<75>Se were examined in the brain of 1-, 4-, 8-, 21- and 56-day-old mice, and evaluated in terms of brain concentration (part per million, ppm) and brain uptake rate (the radioactivity percentage of injected dose per gram of brain, %dose/g), respectively. As a result, the brain concentrations of Mn was increasing with growth, although those of Se and Zn were hardly changing. On the other hand, the uptakes of the 3 tracers by the brain of 1-day-old mice were much higher than those of other elder ones. Using radioactive ^<54>Mn as a single tracer, autoradiography was examined to known the Mn uptake regional distribution in the brain of 1-, 8- and 21-day-old mice, and observed higher regional uptake of Mn by the cerebral cortex, hippocampus, thalamus and hypothalamus in the young brain. Less

进行研究是为了更好地获取术的技术和弹性中子激活分析。 ^<46> sc， ^<54> Mn， ^<58> fe， ^<58> co， ^<65> zn， ^<75> se， ^<75> se， ^<83> rb和 ^<88>的比较摄取行为在11个器官（脑）和血液中检查了ZR示踪剂，并根据“组织摄取速率（组织的放射性，％剂量/g）进行评估。多功能技术的特征摄取，SC，MN，MN，MN，MN，MN，MN，MN，大脑和其他器官的Fe，Co，Zn，Se，Rb和Zn，作为独特的，每个元素在Brai ... Ults中的积累大脑。2）大脑区域吸收 ^<46> sc， ^<54> mn， ^<58> co， ^<65> zn， ^<83> rb在小鼠中使用多态在不足的状态下必不可少的锰，锌或硒。发现较高的状态元素从0.4ppm到300.4ppm。发现多阶段技术非常筛选元素相互关系。3）锰（MN），锌（Zn），锌（Zn）和selenium（SE）的浓度在4-，8-，21-和56的大脑中检查了融化技术，中子激活分析和自显影术和示踪剂的摄取行为。 - 和56岁和56岁的老鼠，并根据脑浓度（PPM）和脑摄取率百分比的脑部注射剂量perram的百分比进行评估，剂量％/g），MN的脑浓度随着生长而增加，Alltoot另一只手很少，1天大的三个示踪剂的吸收量很高。并观察到年轻大脑中大脑皮质，海马，丘脑和降期对MN的较高区域摄取。