Bioinorganic chemical studies on behavior of selenium and other metals by the use of multielemental analysis.

利用多元素分析对硒和其他金属的行为进行生物无机化学研究。

基本信息

批准号：
07640742
负责人：
AMANO Ryohei
金额：
$ 1.47万
依托单位：
Kanazawa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07640742/
关键词：
multitracer experiment neutron activation analysis trace element, V,Mn, Rb regional cerebral tracer uptake regional cerebral concentration brain function age effect mouse experiment マルチトレーサ法中性子放射化分析法脳機能ラジオアイソトープセレンバナジウム骨集

项目摘要

This study was undertaken in order to acquire better understanding of the biobehavior of selenium and other trace metals by the techniques of multitracer and instrumental neutron activation analysis. The followings were found.1). The radioactive multitracer technique, which was established at RIKEN,enables simultaneous tracing of a number of elements in a variety of bio-systems and an accurate comparison of their biobehavior. In this work, the multitracer technique was thus shown to be useful for comparative studies of behavior of trace elements in living bodies.2). We find new facts using the radioactive multitracer technique. (a) V is a bone-seeking element probably playing an role in bone metabolism. (b) Rubidium uptake rates for all soft tissues of mice housed under a 10% O_2 atmosphere were found to be higher than those of the tissues of mice housed under the other three O_2 atmospheres (20%, 80%, and 100%), but no difference was observed for the other elements (Sc, Mn, Fe, Co, Se, etc.) under any of O_2 the atmospheres. (c) Regional cerebral uptake behaviors of Mn, Fe, Zn and Se trace elements were determined in normal C57BL/6N mice, by using a quadruple radioactive tracers. In all regions the uptake rates were found in the following order ; Zn > Mn > Fe >Se.3). Form the technique of instrumental neutron activation analysis for small amount of regional samples. No difference in regional concentration was found in the brain of young and aged mice for the elements, Na, Mg, Cl, K,Rb and Br. On the other hand, significant differences were found for the element, Mn, in all regions of the brain of young and aged mice.

为了更好地了解硒和其他痕量金属的生物行为，通过多阶段和仪器中子激活分析，进行了这项研究。找到以下内容。1）。在Riken建立的放射性多阶段技术可以同时追踪各种生物系统中的许多元素，并准确比较其生物obehavior。因此，在这项工作中，多阶段技术被证明可用于对生物中微量元素的行为的比较研究。2）。我们使用放射性多阶段技术发现了新事实。（a）V是寻求骨骼的元素，可能在骨骼代谢中起作用。（b）发现容纳在10％O_2大气下的小鼠的所有软组织的Rubidium摄取率高于其他三个O_2大气中饲养的小鼠组织的rubistue率（20％，80％和100％），但是在O_2的任何一个大气中，其他元素（SC，MN，FE，CO，SE等）都没有观察到差异。（c）通过使用四倍的放射性示踪剂，在正常的C57BL/6N小鼠中确定Mn，Fe，Zn和SE痕量元件的区域脑摄取行为。在所有地区，都按以下顺序找到吸收率； Zn> Mn> fe> se.3）。形成少量区域样品的仪器中子激活分析的技术。对于元素，Na，mg，cl，k，rb和br，在年轻小鼠和老年小鼠的大脑中没有发现区域浓度的差异。另一方面，在年轻小鼠和老年小鼠大脑的所有区域中发现了MN元素的显着差异。