Brain regional distributions of some transient metals and their bioinorganic chemical studies by the use of multielemental analysis.

使用多元素分析研究一些瞬态金属的脑区域分布及其生物无机化学研究。

• 批准号: 14540512
• 负责人: AMANO Ryohei
• 金额: $ 1.98万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14540512/
• 关键词: Multitracer experiment; neutron activation analysis; ICP-MS analysis; bio-trace element; regional brain concentration; brain function; age effect; brain development; マルチトレーサ法; 中性子放射化分析; ICP-MS; 脳; MRI; マンガン; 鉄; 生物無機化学; 亜鉛; 銅; 生物機化学

This study was undertaken in order to acquire better understanding of the brain regional biobehavior of some transient metals and their bioinorganic chemical studies by the nuclear probe techniques of the multitracer. The followings were found. 1).Mn, Fe, Cu and Zn concentrations were determined in the brain regions of normal Wistar rats using inductively coupled plasma mass spectrometry (ICP-MS), and their maps were illustrated to compare visually the distribution of the elements at the stage of the growth process. Among new findings about their brain distribution, it is especially worth noting that high concentrations of Mn, Fe and Zn were observed in the substantia nigra compared with those in neighboring regions. 2)Using the above technique, the changes in Mn, Fe, Cu and Zn concentrations were also examined in the brain of a 6-hydroxydopamine (OHDA)-induced rat model of Parkinson's disease. The results indicated that Mn, Fe, Cu and Zn levels were increased in the 6-OHDA-lesioned substantia nigra, amygdala and putamen. A marked increase in Fe level was observed in the substantia nigra of model rats. The increase in manganese level occurred earlier than those in the other elements reaching a plateau in the 6-OHDA brain, especially in the substantia nigra. The patterns in these 4 elements may hold the key to the development of Parkinson's disease. 3)We observed the direct transport of radioactive ^<86>Rb^+ and ^<201>Tl^+ via the olfactory nerve transport following intranasal administration in mice. These results evidently indicated the axonal transport of the via the olfactory nerve pathway, from nasal cavity to the olfactory bulb, and moreover to the olfactory cortex through the synaptic junctions. The olfactory transport of the ^<86>Rb^+ and ^<201>Tl^+ should represent the behavior of K^+ in the olfactory system.

为了更好地了解某些瞬时金属的大脑区域生物行为，并通过多阶段的核探测技术对大脑区域生物行为进行了更好的了解。发现了以下内容。 1）.MN，Fe，Cu和Zn浓度在正常Wistar大鼠的大脑区域使用电感耦合的血浆质谱法（ICP-MS）确定，并说明了它们的地图以比较生长过程阶段的元素分布。在有关其大脑分布的新发现中，尤其值得注意的是，与邻近地区相比，在黑质中观察到了高浓度的Mn，Fe和Zn。 2）使用上述技术，还检查了Mn，Fe，Cu和Zn浓度的变化在6-羟基羟基胺（OHDA）诱导的帕金森氏病大鼠模型的大脑中。结果表明，在6- OHDA渗透的黑质中，Mn，Fe，Cu和Zn水平升高。在模型大鼠的黑质中观察到了Fe水平的显着升高。锰水平的增加发生在其他元素中，在6-OHDA大脑中，尤其是在黑质的Nigra中达到高原的锰水平。这4个要素中的模式可能是帕金森氏病发展的关键。 3）我们通过小鼠鼻内给药后的嗅觉神经传输观察到放射性 ^<86> rb ^+和 ^<201> tl ^+的直接运输。这些结果显然表明通过嗅觉神经途径，从鼻腔到嗅球的轴突运输，再到嗅皮层通过突触连接。 ^<86> rb^+和^<201> tl^+的嗅觉传输应代表嗅觉系统中K^+的行为。

项目成果

Brain regional distribution of the minor and trace elements, Na, K, So, Cr, Mn, Co, Zn and Se, in mice bred under Zn-deficient, -adequate and -excessive diets

缺锌、充足和过量饮食下饲养的小鼠中微量元素和微量元素 Na、K、So、Cr、Mn、Co、Zn 和 Se 的脑区域分布

• 发表时间: 2003
• 期刊: J.Radioanal.Nucl.Chem. vol.257(2)
• 作者: Yuko Yabushita;Yousuke Kanayama;Tohru Tarohda;Takae Tuji;Koshin Washiyama;Ryohei Amano
• 通讯作者: Ryohei Amano

Multitracer screening : Brain delivery of trace elements by eight different administration method

多重示踪剂筛选：通过八种不同的给药方法向大脑输送微量元素

• 发表时间: 2005
• 期刊: BioMetals (印刷中)
• 作者: Y.Kanayama;T.Tsuji;S.Enomoto;R.Amano
• 通讯作者: R.Amano

Yabushita Y, Kanayama Y, Tarohda T, Tuji T, Washiyama K, Enomoto S, Amano R: "Brain regional distribution of the minor and trace elements, Na, K, Sc, Cr, Mn, Co, Zn and Se, in mice bred under Zn-deficient, -adequate and -excessive diets"Journal of Radioan

Yabushita Y、Kanayama Y、Tarohda T、Tuji T、Washiyama K、Enomoto S、Amano R：“小鼠中微量元素和微量元素 Na、K、Sc、Cr、Mn、Co、Zn 和 Se 的脑区域分布

Yabushita Y, Kanayama Y, Tarohda T, Tuji T, Washiyama K, Enomoto S. Amano R: "Brain regional distribution of the minor and trace elements, Na, K, Sc, Cr, Mn, Co, Zn and Se, in mice bred under Zn-deficient, -adequate and -excessive diets"Journal of Radioan

Yabushita Y、Kanayama Y、Tarohda T、Tuji T、Washiyama K、Enomoto S. Amano R：“小鼠中微量元素和微量元素 Na、K、Sc、Cr、Mn、Co、Zn 和 Se 的脑区域分布

Yabushita Y, Kanayama Y, Tarohda T, Enomoto S, Amano R: "Multitracer screening for in vivo element-element interrelations Part II : Uptake of ^<65>Zn, ^<46>Sc, ^<58>Co ^<54>Mn, ^<75>Se, ^<83>Rb, ^<85>Sr and ^<88>Zr by brain and other organs of mice bred u

Yabushita Y、Kanayama Y、Tarohda T、Enomoto S、Amano R：“体内元素-元素相互关系的多重示踪剂筛选第二部分：^<65>Zn、^<46>Sc、^<58>Co ^<54 的摄取