Analysis of sweating of human skin transplanted on the weak immune mouse

免疫弱小鼠移植人体皮肤出汗分析

基本信息

批准号：
10470018
负责人：
KOSAKA Mitsuo
金额：
$ 8.38万
依托单位：
Institute of Tropical Medicine, Nagasaki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10470018/
关键词：
Method on research for sweating nude-SCID mouse Weak immune mouse Human skin transplantation Catecholamine VIP Ionto-phrosis QSART (Quantative Sudo-Motor Axon Reflex)NMR-MRI QSART定量的軸索反射性発汗テストコリン作動性汗腺交感神経支配アセチールコリンアドレナリン V

项目摘要

The heterologous, human skin graft provides a novel moded permitting, independent of the normal sweat gland innervation, the analysis of molecular receptors of sweat gland cells by which the actions of natural transmitters and pharmacological agents are transduced.(1) The experimental results of 1999 combined with these of 1998Experimental series I (16 experiments on 16 subjects). Acetylcholine iontophoresed at 100mg/ml standard concentration was compared with nicotine, pilocarpine, adrenaline and with vasoactive intestinal polypeptide (VIP) at 3 different concentrations (0.06, 0.36, 1.10 mg/ml). Cholinergic agonists induced both direct sweating reflex (DIR) and sudomotor axon reflex (AXR) Adrenaline induced a weak DIR but no AXR. With the 2 higher VIP concentrations DIR but no AXR was produced, and skin temperatures increased slightly.(2) Experimental series II (6 experiments on 6 subjects). After a first VIP iontophoresis at (0.36mg/ml) which elicited DIR but not AXR. The subjects exercised for 30 min to induce sweating. After 60 min rest , sweating had disappeared and heart rate was normal, but temperatures remained elevated (oral temperature by 0.22℃, skin temperatures by 0.67℃) indicating a thermoregulatory stated favoring heat dissipation. VIP iontophoresis now caused larger rises of local skin temperature, elicited weak but distinct AXR with a latency of about 2.5 min and an augmented DIR.(3) High-field micro imaging has advantages of higher sensitivity and higher spatial resolution, and T1- and T2- weighted images offer good contrasts which is able to use mouse as a standard animal model for physiological and pathological studies.

异源，人类的皮肤移植物提供了一种新颖的允许，分子的分析是天然发射器的作用，并与1998年经验序列I一起转导的药理学剂。，肾上腺素和血管活性多肽（VIP）在3种不同的浓度（0.06，0.36，1.10 mg /ml） VIP浓度DIR但无AXR产生2）实验级数II（6个受试者的6个实验）。心率是正常的，但是温度升高（口服温度0.22°C，皮肤温度降低0.67°C），有利于热量耗散的速度图像提供了良好的对比度，能够将小鼠用作用于生理和病理研究的标准动物模型。