Analysis of sweating of human skin transplanted on the weak immune mouse

免疫弱小鼠移植人体皮肤出汗分析

基本信息

批准号：
10470018
负责人：
KOSAKA Mitsuo
金额：
$ 8.38万
依托单位：
Institute of Tropical Medicine, Nagasaki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10470018/
关键词：
Method on research for sweating nude-SCID mouse Weak immune mouse Human skin transplantation Catecholamine VIP Ionto-phrosis QSART (Quantative Sudo-Motor Axon Reflex)NMR-MRI QSART定量的軸索反射性発汗テストコリン作動性汗腺交感神経支配アセチールコリンアドレナリン V

项目摘要

The heterologous, human skin graft provides a novel moded permitting, independent of the normal sweat gland innervation, the analysis of molecular receptors of sweat gland cells by which the actions of natural transmitters and pharmacological agents are transduced.(1) The experimental results of 1999 combined with these of 1998Experimental series I (16 experiments on 16 subjects). Acetylcholine iontophoresed at 100mg/ml standard concentration was compared with nicotine, pilocarpine, adrenaline and with vasoactive intestinal polypeptide (VIP) at 3 different concentrations (0.06, 0.36, 1.10 mg/ml). Cholinergic agonists induced both direct sweating reflex (DIR) and sudomotor axon reflex (AXR) Adrenaline induced a weak DIR but no AXR. With the 2 higher VIP concentrations DIR but no AXR was produced, and skin temperatures increased slightly.(2) Experimental series II (6 experiments on 6 subjects). After a first VIP iontophoresis at (0.36mg/ml) which elicited DIR but not AXR. The subjects exercised for 30 min to induce sweating. After 60 min rest , sweating had disappeared and heart rate was normal, but temperatures remained elevated (oral temperature by 0.22℃, skin temperatures by 0.67℃) indicating a thermoregulatory stated favoring heat dissipation. VIP iontophoresis now caused larger rises of local skin temperature, elicited weak but distinct AXR with a latency of about 2.5 min and an augmented DIR.(3) High-field micro imaging has advantages of higher sensitivity and higher spatial resolution, and T1- and T2- weighted images offer good contrasts which is able to use mouse as a standard animal model for physiological and pathological studies.

异源的人类皮肤移植物提供了一种新型的调制允许，与正常的汗腺神经支配无关，分析汗腺细胞的分子受体，通过该分子受体，通过该分子受体进行了自然发射器和药物的作用。（1）1999年的实验结果与1998年经验I系列I（16次实验）结合了16个受试者。将以100mg/mL标准浓度的乙酰胆碱离子噬菌体与尼古丁，毛果氨酸，肾上腺素和血管活性肠多肽（VIP）进行比较，以3种不同的浓度（0.06、0.36、0.36、1.10 mg/ml）。胆碱能激动剂诱导直接出汗反射（DIR）和Sudomotor轴突反射（AXR）肾上腺素诱导弱DIR，但没有AXR。使用2个较高的VIP浓度DIR，但没有产生AXR，并且皮肤温度略有升高。（2）实验II（6个受试者的6个实验）。在以（0.36mg/ml）为单位的第一个VIP离子噬菌体后，它引起了DIR但不引起AXR。受试者锻炼30分钟以引起出汗。休息60分钟后，出汗消失了，心率正常，但温度保持升高（口服温度为0.22℃，皮肤温度下降0.67℃），表明热心调节表明有利于散热。现在，VIP离子噬菌体导致局部皮肤温度的升高，引起弱但明显的AXR，其潜伏期约为2.5分钟，并且增强的DIR。（3）高场微成像具有较高的敏感性和较高的空间分辨率，并且T1-和T2加权图像具有良好的对比，可将良好的对比与物理模型一起使用，可用于物理和病理学模型。