Study on sweating from xenotransplanted human skin

异种移植人体皮肤出汗的研究

基本信息

批准号：
08407003
负责人：
KOSAKA Mitsuo
金额：
$ 6.14万
依托单位：
Nagasaki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08407003/
关键词：
Method on research for sweating nude-SCID Mouse Week immune mouse Human skin transplantation Catecholamine VIP Ionto-phrosis QSART (Quantative Sudo-Motor Axon Reflex)アセチール・コリンアドレナリン VIP(vaso-active intestinal polypeptide)ピロカルピン

项目摘要

I.The experimental results of 1996In human skin transplanted to the back of 3 strains of immuno-deficient mice the function of the eccrine sweat glands of the human transplant was tested by topical intradermal application of pilocarpine, adrenaline and atropine + pilocarpine. Sweat responses were obseved in pre-selected fields of obaervation by means of video macroscope. The iodine starch reaction served as an indicator for the appearance of sweat sport and permitted the evaluation of areas wetted by sweat in the field of observation. Among 9 animals tested, the hybrids between the CB-17-scid mouse and the BALB/cA-nu mouse (BALA/cA-nu, scid) seemded to exhibit the most consistent sweating response to local pharmacological stimulation. According to histological examination, eccrine sweat glands were preserved in human skin stansplanted into the back skin of the BALB/cA-nu, scid mouse strain. The heterologous, human skin graft provides a novel moded permitting, independent of the normal … More sweat gland innervation, the analysis of moleculare receptors of sweat gland cells by which the actions of natural transmitters and pharmacological agents are transduced.II.The experimental results of 1997Experimental series I (16 experiments on 16 subjects). Acetylcholine iontophoresed at 100mg/ml standard concentration was compared with nicotine, pilocarpine, adrenaline and with vasoactive intestinal polypeptide (VIP) at 3 different concentrations (0.06,0.36,1.10 mg/ml). Cholinergic agonists induced both direct sweating reflex (DIR) and sudomotor axon reflex (AXR) Adrenaline induced a weak DIR but no AXR.With the 2 higher VIP concentrations DIR but no AXR was produced, and skin temperatures increased slightly.Experimental seriesII (6 experiments on 6 subjects). After a first VIP iontophoresis at (0.36mg/ml) which elicited DIR but not AXR.The subjects exercised for 30 min to induce sweating. After 60 min rest, sweating had disappeard and heart rate was normal, but temperatures remained elevated (oral temperature by 0.22ﾟC,skin temperatures by 0.67ﾟC) indicating a thermoregulatory stated favoring heat dissipation. VIP iontophoresis now caused larger rises of local skin temperature, elicited weak but distinct AXR with a latency of about 2.5 min and an augmented DIR. Less

一、1996年的实验结果在移植到3株免疫缺陷小鼠背部的人体皮肤中，通过局部皮内应用毛果芸香碱、肾上腺素和阿托品+毛果芸香碱的汗腺反应来测试人体移植物的小汗腺功能。通过视频宏观显微镜在预先选择的观察区域中观察碘淀粉反应作为出汗运动出现的指标。在 9 只测试的动物中，CB-17-scid 小鼠和 BALB/cA-nu 小鼠（BALA/cA-nu，scid）之间的杂交小鼠似乎表现出根据组织学检查，植入 BALB/cA-nu、scid 小鼠品系背部皮肤的人皮肤中保留了小汗腺。异源人体皮肤移植提供了一种新颖的修饰允许，独立于正常的汗腺神经支配，对汗腺细胞的分子受体进行分析，通过这些受体来转导自然递质和药物的作用。II.1997年实验的实验结果系列 I（对 16 名受试者进行 16 次实验）将 100mg/ml 标准浓度的乙酰胆碱离子电渗疗法与尼古丁、毛果芸香碱、肾上腺素进行比较。 3种不同浓度的血管活性肠多肽（VIP）（0.06、0.36、1.10 mg/ml）胆碱能激动剂诱导直接出汗反射（DIR）和催汗轴突反射（AXR）。肾上腺素诱导弱DIR，但不诱导AXR。 2个较高的VIP浓度DIR但没有产生AXR，并且皮肤温度略有增加。实验系列II（6个实验对6第一次 VIP 离子电渗疗法（0.36mg/ml）后，引发 DIR，但不引发 AXR。受试者运动 30 分钟以诱导出汗。休息 60 分钟后，出汗消失，心率正常，但体温仍然升高。（口腔温度为 0.22°C，皮肤温度为 0.67°C）表明有利于 VIP 离子电渗疗法的温度调节。现在导致局部皮肤温度大幅上升，引发微弱但明显的 AXR，延迟时间约为 2.5 分钟，DIR 增强。