Morphological study on the pain transmission pathway and its inhibitory mechanism

疼痛传导途径的形态学研究及其抑制机制

基本信息

批准号：
08670012
负责人：
ATSUMI Saoko
金额：
$ 1.41万
依托单位：
YAMANASHI MEDICAL UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670012/
关键词：
spinal cord primary afferent fiber synaptic glomerulus pain enkephalin substance P GABA glycine 免疫細胞化学

项目摘要

The aim of this study is to elucidate the neural circuitry between terminals of nociceptive primary afferents and long ascending tract neurons, and inhibitory control mechanism of pain transmission in the dorsal horn of the chicken by morphological techiniques. The following results were obtained in 1996 and 1997.1. Neural circuitry from primary afferents to long ascending tract cells. (1) Presumable long ascending tract cells which received nemerous substance P (SP)-containing axon terminals were distributed in the laminae I and II throughout the spinal cord and observed once in every 50mum. (2) In chickens which had been given backskin-cut in either side of the body to give chronic pain, time course of SP-immunoreactivity (IR) in the dorsal horn was examined. The SP-IR changed diphasically ; it decreased immediately after skin-cut, then increased, next decreased, and then increased and finally gradually returned to the original level.2. Inhibitory mechanism of pain sensation at the d … More orsal horn level. (1) In the synaptic glomerulus, glycine-containing elements made synapses with the central terminal of the primary afferents. Some glycine terminals coexisted with GABA.(2) Enkephalin (Enk)-containing elements in the synaptic glomerulus were demonstarated to be vesicle-containing dendrites by the double labeling technique using antiMAP2 and antiEnk antibodies. (3) The structure of synaptic glomeruli was examined by the freeze-fracture technique. On the P face of the central terminal, the characteristic feature of presynapic P face (dimples and surrounding intramembrane particles (IMPs)) was observed adjacent to aggregate of IMPs which was characteristic to the postsynaptic membrane, suggesting the presence of reciprocal synapses.3. As to terminals of primary afferents, HRP was injected in the spinal ganglion. Labeled unmyelinated and thin myelinated fibers were examined by electron microscopy. These formed the central terminal in the synaptic glomerulus. A part of the labeled central terminals showed SP-IR. Less

本研究的目的是通过形态学技术阐明鸡的伤害性初级传入神经末梢和长升束神经元之间的神经回路，以及背角疼痛传递的抑制控制机制。1996年和1997年获得了以下结果。 (1) 推测长升束细胞接受了恶性物质P含有 (SP) 的轴突末端分布在整个脊髓的 I 层和 II 层中，每 50 只鸡中观察到一次。 (2) 在身体两侧进行背部皮肤切割以产生慢性疼痛的鸡中。观察背角SP-免疫反应性(IR)的变化过程，切皮后SP-IR立即降低，然后升高，然后降低，然后升高，最后逐渐恢复到原来的水平。 2. 口角水平的痛觉抑制机制 (1) 在突触肾小球中，含甘氨酸的元件与初级传入神经的中央末端形成突触。(2) ）突触肾小球中含有脑啡肽（Enk）的元素被证明是含有囊泡的树突(3)通过冷冻断裂技术检查突触肾小球的结构。在中央末端的P面上，突触前P面的特征（凹坑和周围的膜内颗粒）。观察到IMPs））靠近IMPs聚集体，这是突触后膜的特征，表明存在相互突触。3．通过电子显微镜检查，将 HRP 注射到脊髓神经节中，这些纤维形成了突触肾小球的中央末端。